Figure 3(a). Effects of Hydrogenized water on the Akt insulin signaling pathway in skeletal muscle cells. Skeletal muscle cells were incubated with 0%, 3.125%, 6.25%, 12.5%, 25% and 50% v/v hydrogenized water in buffer for 60 min. Cells were treated with concentrations of 30 nM insulin (Sigma, Aldrich, St. Louis, MO) for 15 min as control and lysed for phospho-Akt (Ser473) and GAPDH Western blotting. (b) The intensity of the Western blotting images shown in Figure 3(b)

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Effects of Hydrogenized Water on Intracellular Biomarkers for Antioxidants, Glucose Uptake, Insulin Signaling and SIRT 1 and Telomerase Activity

Robert Settineri, Jin Ji, Chunlan Luo, Rita R. Ellithorpe, Gonzalo Ferreira de Mattos, Steven Rosenblatt, James LaValle, Antonio Jinenez, Shigeo Ohta, Garth L. Nicolson

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