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From

Effects of Hydrogenized Water on Intracellular Biomarkers for Antioxidants, Glucose Uptake, Insulin Signaling and SIRT 1 and Telomerase Activity

Robert Settineri, Jin Ji, Chunlan Luo, Rita R. Ellithorpe, Gonzalo Ferreira de Mattos, Steven Rosenblatt, James LaValle, Antonio Jinenez, Shigeo Ohta, Garth L. Nicolson

American Journal of Food and Nutrition. 2016, 4(6), 161-168 doi:10.12691/ajfn-4-6-4
  • Figure 1. Permeability of glucose (monitored via the fluorescent derivative of glucose 2-NBDG) across Caco-2 cell monolayers upon incubation with HBSS (control) or 0.25, 0.5, 6.25, 12.5, 25 or 50% v/v hydrogenized water in HBSS in an incubation time of 30 min. *P<0.05
  • Figure 2. Permeability of glucose (monitored via a fluorescent derivative of glucose) across Caco-2 cell monolayers upon incubation with HBSS (control) and 3.125%, 6.25%, 12.5%, 25% and 50% v/v hydrogenized water in HBSS in an incubation time (60 min). *P<0.05
  • Figure 3(a). Effects of Hydrogenized water on the Akt insulin signaling pathway in skeletal muscle cells. Skeletal muscle cells were incubated with 0%, 3.125%, 6.25%, 12.5%, 25% and 50% v/v hydrogenized water in buffer for 60 min. Cells were treated with concentrations of 30 nM insulin (Sigma, Aldrich, St. Louis, MO) for 15 min as control and lysed for phospho-Akt (Ser473) and GAPDH Western blotting. (b) The intensity of the Western blotting images shown in Figure 3(b)
  • Figure 3(b). The intensity of the optical density of the Western blotting images shown in Figure 3(a)
  • Figure 4. Effects of hydrogenized water on SIRT1 expression in human umbilical vein endothelial cells. Human umbilical vein endothelial cells were incubated with hydrogenized water at 0, 3.125, 6.25, 12.5, 25 and 50% v/v for 24 hour. Cell were treated with concentrations of 100 μM Resveratrol for 24 hr as a control. Cells were lysed and lysates prepared for SIRT1 and GAPDH Western blotting
  • Figure 5. The effect of hydrogenized water on SIRT1 expression in human umbilical endothelial cells. The results are expressed as relative densities of the western blot images in Figure 4
  • Figure 6. Effects of Hydrogenized water on Telomerase activity in human umbilical vein endothelial cells (HUVECs). Human umbilical vein endothelial cells (HUVECs) were exposed to 0%, 3.125%, 6.25%, 12.5%, 25%, 50% v/v hydrogenized water in bufer, and 10 nM raloxifene for 24 hr. 10 nM raloxifene, a known telomerase activity up-regulator, is used as a reference material and positive control. The telomerase activity data are normalized against that of the control cells.