Figure 5. Puerarin attenuated TGF-β1-induced Smad expression in NRCMs NRCMs were transfected with 1 μL of Smad2siRNA. After 24 h of transfection, untransfected cells were treated with TGF-β1 (3 μg/L) individually or in combination with puerarin (1 g/L) for 24 h, and Smad2siRNA-transfected cells were treated with TGF-β1 (3 μg/L) for 24 h. Untreated cells were used as a control. mRNA (A) and protein (B) expression of Smad2, pSmad2, and total Smad2/3 were determined using qRT-PCR and Western blot analysis, respectively. (C) Quantification of (B). Data are expressed as mean ± SD. *P < 0.05 vs. control; #P < 0.01 vs. TGF-β1 group; &P < 0.05 vs. puerarin group (n=6) : Puerarin Attenuates Transforming Growth Factor Beta-1 Induced Hypertrophic Responses and Smad Proetin Upregulation in Neonatal Rat Cardiomyocytes : Science and Education Publishing

Figure 5. Puerarin attenuated TGF-β_1-induced Smad expression in NRCMs NRCMs were transfected with 1 μL of Smad2siRNA. After 24 h of transfection, untransfected cells were treated with TGF-β₁ (3 μg/L) individually or in combination with puerarin (1 g/L) for 24 h, and Smad2siRNA-transfected cells were treated with TGF-β₁(3 μg/L) for 24 h. Untreated cells were used as a control. mRNA (A) and protein (B) expression of Smad2, pSmad2, and total Smad2/3 were determined using qRT-PCR and Western blot analysis, respectively. (C) Quantification of (B). Data are expressed as mean ± SD. *P < 0.05 vs. control; #P < 0.01 vs. TGF-β1 group; &P < 0.05 vs. puerarin group (n=6)

American Journal of Biomedical Research. 2020, 8(2), 40-46 doi:10.12691/ajbr-8-2-3