Figure 4. Puerarin abrogated TGF-β1-induced hypertrophic response in NRCMs. (A) NRCMs were treated with different doses of puerarin (0.1, 1, or 5 g/L) for 24 h in the presence of TGF-β1 (3 μg/L). Protein synthesis in NRCMs was evaluated by measuring [³H]-leucine incorporation. (B) NRCMs were transfected with different amounts of Smad2siRNA (0.6, 1, or 1.4 μL). After 24 h of transfection, untransfected cells were treated with TGF-β1 (3 μg/L) individually or in combination with puerarin (1 g/L) for 24 h, and Smad2siRNA-transfected NRCMs were treated with TGF-β1 (3 μg/L) for 24 h. Protein synthesis was evaluated by measuring [³H]-leucine incorporation. (C) NRCMs were transfected with 1 μL of Smad2siRNA. After 24 h of transfection, untransfected cells were treated with TGF-β1 (3 μg/L) individually or in combination with puerarin (1 g/L) for 24 h, and Smad2siRNA -transfected cells were treated with TGF-β1 (3 μg/L) for 24 h. The mRNA levels of ANF and β-MHC were determined using qRT-PCR. GAPDH was used as an internal control. Data are expressed as mean ± SD. *P<0.05vs.control; #P<0.01vs.TGF-β₁ group; &P<0.05vs. puerarin group (n=6)

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Puerarin Attenuates Transforming Growth Factor Beta-1 Induced Hypertrophic Responses and Smad Proetin Upregulation in Neonatal Rat Cardiomyocytes

Yongying Shi, Aijun Liu, Junjiang Lu, Guangyuan Chen, Shiming Liu, Minsheng Chen, Chengfeng Luo

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