Figure 2. Smad protein expression in TGF-β1-stimulated NRCMs. NRCMs were treated with TGF-β1 (3 μg/L) for 15 min, 30 min, 1 h, 2 h, or 24 h. (A) The protein expressions of Smad2, phospho-Smad2 (pSmad2; Ser465/467), and total Smad2/3 were determined using Western blot analysis. (B) Quantification of (A). β-actin was used as an internal control. Data are expressed as mean ± SD. *P < 0.05 vs. control (n=7)

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Puerarin Attenuates Transforming Growth Factor Beta-1 Induced Hypertrophic Responses and Smad Proetin Upregulation in Neonatal Rat Cardiomyocytes

Yongying Shi, Aijun Liu, Junjiang Lu, Guangyuan Chen, Shiming Liu, Minsheng Chen, Chengfeng Luo

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