Figure 1. Dosage optimization of TGF-β₁ to induce hypertrophic responses in primary neonatal rat cardiomyocytes (NRCMs). NRCMs were seeded at a density of 10⁶ cells/well in 24-well plates and cultured overnight. After a 24- h incubation in serum-free medium, NRCMs were stimulated with 0, 0.1, 1, 3, or 10 μg/L of TGF-β₁ for 24 h. (A–F) Cellular RNA was stained with RNA-sensitive fluorescence probe propidium iodide (PI) after DNase treatment. Representative images are shown in (A–E). Scale bar: 100 μm. The fluorescent signals were measured in five randomly selected fields in each well (F). (G) Protein synthesis was evaluated by measuring the incorporation of [³H]-leucine. The radioactivity of [³H]-leucine incorporated into the trichloroacetic acid precipitable material was determined by β-scintillation counting. The mRNA levels of atrial natriuretic factor (ANF) (H) and β-myosin heavy chain (β-MHC) (I) were determined using quantitative real-time PCR (qRT-PCR). GAPDH was used as an internal control. Data are expressed as mean ± standard deviation (SD). ^*P < 0.05 vs. control; ^#P < 0.05 vs. 3 μg/L TGF-β₁ (n=7).

From

Puerarin Attenuates Transforming Growth Factor Beta-1 Induced Hypertrophic Responses and Smad Proetin Upregulation in Neonatal Rat Cardiomyocytes

Yongying Shi, Aijun Liu, Junjiang Lu, Guangyuan Chen, Shiming Liu, Minsheng Chen, Chengfeng Luo

• Figure 1 of 5  (Figures index)
• Next