A Convenient Synthesis of Ethyl 1-Amino-3-(Substituted Phenyl)-2-Cyano-3H-Benzo[4,5]Thiazolo-...

Hany M. Mohamed

  Open Access OPEN ACCESS  Peer Reviewed PEER-REVIEWED

A Convenient Synthesis of Ethyl 1-Amino-3-(Substituted Phenyl)-2-Cyano-3H-Benzo[4,5]Thiazolo-[3,2-a]Pyridine-4-Carboxylate Derivatives and Some of their Reactions

Hany M. Mohamed1,2,

1Chemistry Department, Faculty of Medicine, Jazan University, Jazan, Saudi Arabia
2Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

Abstract

The titled compounds were prepared by interaction of ethyl 2-(benzo[d]thazol-2-yl)acetate (3) with different arylidinemalononitrile derivatives (4a-c) in EtOH/TEA solution at room temperature. When 3 was treated with 2-(ethoxymethylene)-malononitrile (4f) under same reaction conditions, the ethyl iminothiazolopyridine-4-carboxylate (6) was obtained. Ethyl (amino(methoxy)methyl)-3-(substitutedphenyl)-1-oxo-1H-benzo[4,5]thiazole[3,2-a]pyridine-4-carboxylate (8a,b) was obtained from reaction of 3 with different cyanoacrylate derivatives (7a,b) in MeOH/TEA at room temperature, while the diethyl thiazolo[3,2-a]pyridine-4-carboxylate derivative (8c) was obtained under same reaction conditions. The amino-imino derivative (10) was also prepared and used to synthesis new pyrido[3,2-e][1,2,4]triaziolo[1,5-c]pyrimidine-5-carboxylate derivatives (12). The structures of all the newly synthesized compounds were confirmed based on their elemental analysis and spectroscopic data.

At a glance: Figures

Cite this article:

  • Mohamed, Hany M.. "A Convenient Synthesis of Ethyl 1-Amino-3-(Substituted Phenyl)-2-Cyano-3H-Benzo[4,5]Thiazolo-[3,2-a]Pyridine-4-Carboxylate Derivatives and Some of their Reactions." World Journal of Organic Chemistry 2.1 (2014): 1-8.
  • Mohamed, H. M. (2014). A Convenient Synthesis of Ethyl 1-Amino-3-(Substituted Phenyl)-2-Cyano-3H-Benzo[4,5]Thiazolo-[3,2-a]Pyridine-4-Carboxylate Derivatives and Some of their Reactions. World Journal of Organic Chemistry, 2(1), 1-8.
  • Mohamed, Hany M.. "A Convenient Synthesis of Ethyl 1-Amino-3-(Substituted Phenyl)-2-Cyano-3H-Benzo[4,5]Thiazolo-[3,2-a]Pyridine-4-Carboxylate Derivatives and Some of their Reactions." World Journal of Organic Chemistry 2, no. 1 (2014): 1-8.

Import into BibTeX Import into EndNote Import into RefMan Import into RefWorks

1. Introduction

The twentieth century has been characterized both by a drastic reduction in the mortality caused by infectious diseases and by a rise in the control of neoplastic pathologies. Nevertheless, microorganism and viruses, on the one hand, and tumors, on the other hand, still represent a dreadful menace to men's health and therefore, for a more efficient control, require the steady development of novel and more powerful drugs.

Benzothiazole derivatives possess a wide spectrum of biological applications such as antitumor [1, 2, 3, 4], antimicrobial [5, 6, 7, 8], schictosomicidal [9], anti-inflammatory [10, 11, 12, 13], anticonvulsants [14, 15], antidiabetic [16, 17], antipsychotic [18] and diuretic [19]. Due to these biological activities, the synthesis of benzothiazole is a considerable area of current discussion. The classical method involves condensation of o-aminothiophenols with substituted aldehydes [20, 21], acyl chlorides, carboxylic acids [22, 23] or esters, nitriles [24]. Other most commonly used methods include Pd/Cu/Mn/chloranil catalyzed cyclization of o-halothioformanilides [25, 26]. To this end, as a continuation of a research program on synthesis of heterocyclic compounds with potential biological activity [27-34][27], I decided to study the synthetic potentiality of ethyl 2-(benzo[d]thiazol-2-yl)acetate (3) [35] towards some reagents as a convenient route to condensed and substituted benzothiazole.

2. Experimental

2.1. Chemicals and Instruments

Melting points were determined on a Stuart melting point apparatus and are uncorrected. IR spectrawere recorded in KBr using a FT-IR 5300 spectrometer and Perkin Elmer spectrum RXIFT-IR system (ν, cm−1). The 1H NMR at (300 MHz) and 13C NMR spectra (75 MHz) were recorded in DMSO-d6on a Varian Mercury VX-300 NMR spectrometer. Chemical shifts (δ) are related to that of thesolvent. Mass spectra were measured on a Shimadzu GMMS-QP-1000 EX mass spectrometer at 70 eV. The elemental analyses were performed at the Microanalytical Center, Cairo University, Cairo (Egypt).

2.2. Starting Materials

Ethyl 2-(benzo[d]thiazol-2-yl)acetate (3) was prepared according to previously reported procedures [35]. All other chemicals used inthis study were commercially available.

2.3. Typical Procedure for Synthesis of (5a-e)

A Solution of ethyl 2-(benzo[d]thiazol-2-yl)acetate (3) (2.21 g, 10 mmol) in ethanol was added to mixture of ethanolic solution of the appropriate arylidinemalononitrile (4a-e) (10 mmol) and triethyl amine (TEA). The mixture was stirred at room temperature for 3-4 hr. until complete precipitation. The solid formed was filtered off, washed by ethanol and recrystalized from ethanol-benzene mixture (1:1) to give compound 5.


2.3.1. Ethyl 1-amino-2-cyano-3-phenyl-3H-benzo[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (5a)

Yellow; Mp 210-212°C; FTIR (KBr, cm-1): 3430, 3337 (NH2), 2186 (CN), 1668 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.20 (t, 3H, CH3CH2), 4.11-4.17 (q, 2H, CH3CH2), 4.16 (s, 1H, 3H-pyridine), 6.49 (brs, 2H, NH2; cancelled by D2O), 7.15-7.38 (m, 7H, Ar-H), 7.73-7.75 (d, 1H, Ar-H), 7.83-7.88 (d, 1H, Ar-H) ppm. MS m/z 375 (M+, 30), 298 (100). Anal. Calcd for C21H17N3O2S: C, 67.18; H, 4.56; N, 11.19. Found: C, 67.21; H, 4.54; N, 11.18.


2.3.2. Ethyl 1-amino-3-(4-chlorophenyl)-2-cyano-3H-benzo[4,5]thiazolo[3,2-a]-pyridine-4-carboxylate (5b)

Yellow; Mp 198-200°C; FTIR (KBr, cm-1): 3420, 3325 (NH2), 2221 (CN), 1667 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.19 (t, 3H, CH3CH2), 4.12-4.15 (q, 2H, CH3CH2), 4.63 (s, 1H, 3H-pyridine), 6.53 (brs, 2H, NH2; cancelled by D2O), 7.16-7.37 (m, 6H, Ar-H), 7.73-7.76 (d, 1H, Ar-H), 7.85-7.88 (d, 1H, Ar-H) ppm. MS m/z 409 (M+, 37), 298 (100). Anal. Calcd for C21H16ClN3O2S: C, 61.53; H, 3.93; N, 10.25. Found: C, 61.51; H, 3.92; N, 10.27.


2.3.3. Ethyl 1-amino-2-cyano-3-(4-methoxyphenyl)-3H-benzo[4,5]thiazolo[3,2-a]-pyridine-4-carboxylate (5c)

Yellow; Mp 175-177°C; FTIR (KBr, cm-1): 3432, 3342 (NH2), 2188 (CN), 1666 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.20 (t, 3H, CH3CH2), 3.67 (s, 3H, OCH3), 4.13 (q, 2H, CH3CH2), 4.54 (s, 1H, 3H-pyridine), 6.48 (brs, 2H, NH2; cancelled by D2O), 6.82-6.85 (d, 2H, Ar-H), 7.05-7.08 (d, 2H, Ar-H), 7.23 (t, 1H, Ar-H), 7.34 (t, 1H, Ar-H), 7.71-7.74 (d, 1H, Ar-H), 7.85-7.88 (d, 1H, Ar-H); 13C NMR (75 MHz, DMSO-d6): 15.45, 36.43, 56.18, 61.42, 68.21, 99.71, 115.31, 117.76, 121.53, 123.49, 123.92, 125.49, 127.43, 128.57, 137.50, 138.03, 151.46, 151.86, 159.43, 164.23. MS m/z 405 (M+, 32), 298 (100). Anal. Calcd for C22H19N3O3S: C, 65.17; H, 4.72; N, 10.36. Found: C, 65.18; H, 4.74; N, 10.38 ppm.


2.3.4. Ethyl 1-amino-2-cyano-3-(4-hydroxyphenyl)-3H-benzo[4,5]thiazolo[3,2-a]-pyridine-4-carboxylate (5d)

Greenish yellow; Mp 224-226°C; FTIR (KBr, cm-1): 3455 (OH), 3415, 3365 (NH2), 2252 (CN), 1669 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.25 (t, 3H, CH3CH2), 4.19 (q, 2H, CH3CH2), 4.51 (s, 1H, 3H-pyridine), 6.55 (brs, 2H, NH2; cancelled by D2O), 6.57-6.59 (d, 1H, Ar-H), 6.76-6.80 (d, 1H, Ar-H), 6.95 (s, 1H, Ar-H), 7.34 (t, 1H, Ar-H), 7.39 (t, 1H, Ar-H), 7.82-7.85 (d, 1H, Ar-H), 7.96-7.99 (d, 1H, Ar-H), 8.90 (s, 1H, OH; cancelled by D2O) ppm. MS m/z 391 (M+, 39), 298 (100). Anal. Calcd for C21H17N3O3S: C, 64.43; H, 4.38; N, 10.73. Found: C, 64.45; H, 4.40; N, 10.74.


2.3.5. Ethyl 1-amino-2-cyano-3-(3-hydroxy-4-methoxyphenyl)-3H-benzo[4,5]thia-zolo[3,2-a]pyridine-4-carboxylate (5e)

Yellow; Mp 218-220°C; FTIR (KBr, cm-1): 3461 (OH), 3402, 3366 (NH2), 2220 (CN), 1666 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.24 (t, 3H, CH3CH2), 3.66 (s, 3H, OCH3), 4.16 (q, 2H, CH3CH2), 4.52 (s, 1H, 3H-pyridine), 6.45 (brs, 2H, NH2; cancelled by D2O), 6.52-6.54 (d, 1H, Ar-H), 6.66-6.69 (d, 1H, Ar-H), 6.75 (s, 1H, Ar-H), 7.23 (t, 1H, Ar-H), 7.35 (t, 1H, Ar-H), 7.71-7.74 (d, 1H, Ar-H), 7.86-7.89 (d, 1H, Ar-H), 8.86 (s, 1H, OH; cancelled by D2O) ppm. MS m/z 421 (M+, 41), 298 (100). Anal. Calcd for C22H19N3O4S: C, 62.69; H, 4.54; N, 9.97. Found: C, 62.71; H, 4.53; N, 9.99.

2.4. Synthesis of 6

A Solution of 3 (2.21 g, 10 mmol) in ethanol was added to mixture of ethanolic solution of ethoxymethylene malononitrile (4f) (1.22 g, 10 mmol) and TEA. The mixture was stirred at room temperature for 4 hr. until complete precipitation. The solid formed was filtered off, washed by ethanol and recrystalized from ethanol to give compound 6.


2.4.1. Ethyl 2-cyano-1-imino-1H-benzo[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (6)

Pal yellow; Mp 188-190°C; FTIR (KBr, cm-1): 3464 (NH), 2218 (CN), 1683 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.33 (t, 3H, CH3CH2), 4.29 (q, 2H, CH3CH2), 7.56-7.61 (m, 2H, Ar-H), 8.07 (s, 1H, CH=pyridine), 8.11-8.13 (d, 1H, Ar-H), 8.32 (brs, 1H, NH; cancelled by D2O), 9.60-9.62 (d, 1H, Ar-H) ppm. MS m/z 297 (M+, 100). Anal. Calcd for C15H11N3O2S: C, 60.59; H, 3.73; N, 14.13. Found: C, 60.57; H, 3.72; N, 14.15.

2.5. Synthesis of Compound 8

Ethyl 2-(benzo[d]thiazol-2-yl)acetate (3) (2.21 g, 10 mmol) and 3-(substituted phenyl)-2-cyanoacrylate (7a-c) (10 mmol) were mixed together in methanolic/TEA solution. The reaction mixture was stirred at room temperature for 4 hr. until complete precipitation. The soled formed was collected and washed with methanol and recrystallized from ethanol/benzene mixture (1:1) to give compound 8.


2.5.1. Ethyl 2-(amino(methoxy)methyl)-1-oxo-3-phenyl-1H-benzo[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (8a)

Pal yellow; Mp 173-175°C; FTIR (KBr, cm-1): 3465, 3373 (NH2), 1671 (CO-ester), 1654 (CO-amide). 1H NMR (DMSO-d6, 300 MHz): 1.21 (t, 3H, CH3CH2), 3.66 (s, 3H, OCH3), 4.17-4.22 (q, 2H, CH3CH2), 5.10 (s, 1H, CH), 7.14-7.28 (m, 6H, Ar-H), 7.37 (t, 1H, Ar-H), 7.55 (brs, 2H, NH2; cancelled by D2O), 7.73-7.76 (d, 1H, Ar-H), 7.85-7.87 (d, 1H, Ar-H) ppm. MS m/z 408 (M+, 49), 331 (100). Anal. Calcd for C22H20N2O4S: C, 64.69; H, 4.94; N, 6.86. Found: C, 64.70; H, 4.96; N, 6.88.


2.5.2. Ethyl 2-(amino(methoxy)methyl)-3-(4-chlorophenyl)-1-oxo-1H-benzo[4,5]thia-zolo[3,2-a]pyridine-4-carboxylate (8b)

Yellowish; Mp 187-189°C; FTIR (KBr, cm-1): 3473, 3399 (NH2), 1671 (CO-ester), 1657 (CO-amide). 1H NMR (DMSO-d6, 300 MHz): 1.23 (t, 3H, CH3CH2), 3.65 (s, 3H, OCH3), 4.17-4.22 (q, 2H, CH3CH2), 5.08 (s, 1H, CH), 7.14-7.17 (d, 2H, Ar-H), 7.26-7.29 (d, 2H, Ar-H), 7.57 (brs, 2H, NH2; cancelled by D2O), 7.35-7.43 (m, 2H, Ar-H), 7.74-7.76 (d, 1H, Ar-H), 7.84-7.87 (d, 1H, Ar-H) ppm. MS m/z 442 (M+, 44), 331 (100). Anal. Calcd for C22H19ClN2O4S: C, 59.66; H, 4.32; N, 6.32. Found: C, 59.68; H, 4.33; N, 6.30.


2.5.3. Diethyl 1-amino-3-(4-methoxyphenyl)-3H-benzo[4,5]thiazolo[3,2-a]pyridine-2,4-dicarboxylate (8c)

Yellow; Mp 204-206 οC; FTIR (KBr, cm-1): 3409, 3306, 3205 (NH2), 1669, 1675 (2CO). 1H NMR (DMSO-d6, 300 MHz): 1.20 (t, 3H, CH3CH2), 1.24 (t, 3H, CH3CH2), 3.65 (s, 3H, OCH3), 4.11 (q, 2H, CH3CH2), 4.18 (q, 2H, CH3CH2), 5.03 (s, 1H, 3H-pyridine), 6.75-6.78 (d, 2H, Ar-H), 7.04-7.07 (d, 2H, Ar-H), 7.25 (t, 1H, Ar-H), 7.36 (t, 1H, Ar-H), 7.49 (brs, 2H, NH2; cancelled by D2O), 7.73-7.76 (d, 1H, Ar-H), 7.84-7.87 (d, 1H, Ar-H) ppm. MS m/z 452 (M+, 36), 345 (100). Anal. Calcd for C24H24N2O5S: C, 63.70; H, 5.35; N, 6.19. Found: C, 63.68; H, 5.36; N, 6.21.

2.6. Synthesis of Compound 9

A mixture of -enaminonitrile 5c (4.05 g, 10 mmol), triethylorthoformate (10 mmol) and Ac2O (30 ml) was refluxed for 3 h. The solvent was removed under reduced pressure and the resulting solid was washed with ethanol, dried and crystallized from benzene to give 9.


2.6.1. Ethyl 2-cyano-1-((ethoxymethylene)amino)-3-(4-methoxyphenyl)-3H-benzo-[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (9)

Yellow; Mp 135-137°C; FTIR (KBr, cm-1): 2202 (CN), 1672 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.16 (t, 3H, CH3CH2), 1.35 (t, 3H, OCH3CH2), 3.72 (s, 3H, OCH3), 4.06-4.13 (m, 2H, CH3CH2), 4.43-4.46 (m , 2H, OCH3CH2), 4.73 (s, 1H, 3H-pyridine), 6.88-6.91 (d, 2H, Ar-H), 7.21-7.24 (m, 3H, Ar-H), 7.28-7.34 (t, 1H, Ar-H), 7.68-7.70 (d, 1H, Ar-H), 7.77-7.80 (d, 1H, Ar-H); 13C NMR (75 MHz, DMSO-d6): 14.19, 14.21, 36.97, 55.08, 60.26, 64.59, 80.04, 96.15, 114.11, 117.01, 119.20, 122.47. 124.39, 126.18, 126.71, 128.22, 136.34, 136.64, 150.14, 151.65, 158.56, 165.76 ppm. MS m/z 461 (M+, 33), 354 (100). Anal.Calcd for C25H23N3O4S: C, 65.06; H, 5.02; N, 9.10. Found: C, 65.10; H, 5.06; N, 9.08.

2.7. Reaction with Anime Derivatives

A mixture of 9 (4.61 g, 10 mmol), hydrazine hydrate, 2-aminoethanol, ethane-1,2-diamine, or propane-1,3-diamine (10 mmol) in EtOH was stirred at room temperature for 4 hr. The solid formed was collected and washed by ethanol. All compounds were recrystallized from dioxan to give compound 10.


2.7.1. Ethyl 3-amino-4-imino-5-(4-methoxyphenyl)-4,5-dihydro-3H-benzo[4',5']thia-zolo[3',2':1,6]pyrido[2,3-d]pyrimidine-6-carboxylate (10a)

Pal yellow; Mp 203-205°C; FTIR (KBr, cm-1): 3322, 3315, 3300, 3266 (NH2 and NH), 1672 (CO). 1H NMR (DMSO-d6, 300 MHz): 1.23 (t, 3H, CH3CH2), 3.66 (s, 3H, OCH3), 4.09-4.16 (q, 2H, CH3CH2), 5.18 (brs, 2H, NH2; cancelled by D2O), 5.76 (s, 1H, 5H-pyridine), 6.77-6.80 (d, 2H, Ar-H), 6.98 (brs, 1H, NH; cancelled by D2O), 7.21-7.36 (m, 4H, Ar-H), 7.67-7.70 (d, 1H, Ar-H), 8.19 (s, 1H, CH-pyrimidine), 8.53-8.56 (d, 1H, Ar-H); 13C NMR (75 MHz, DMSO-d6): 14.31, 36.72, 54.95, 59.94, 96.04, 113.52, 118.22, 122.03, 123.74, 125.88, 126.82, 128.61, 136.70, 137.19, 145.62, 150.63, 158.03, 166.03 ppm. MS m/z 447 447 (M+, 41), 340 (100). Anal. Calcd for C23H21N5O3S: C, 61.73; H, 4.73; N, 15.65. Found: C, 61.74; H, 4.75; N, 15.67.


2.7.2. Ethyl 3-(2-hydroxyethyl)-4-imino-5-(4-methoxyphenyl)-4,5-dihydro-3H-benzo-[4',5']thiazolo[3',2':1,6]pyrido[2,3-d]pyrimidine-6-carboxylate (10b)

Yellowish; Mp 192-194°C; FTIR (KBr, cm-1): 3443 (OH), 3335, 3318, 3330, 3266 (NH2 and NH), 1666 (CO). 1H NMR (DMSO-d6, 300 MHz): 1.25 (t, 3H, CH3CH2), 3.53 (t, 2H, CH2-N), 3.67 (s, 3H, OCH3), 3.98 (t, 2H, CH2OH), 4.09-4.17 (q, 2H, CH3CH2), 5.04 (s, 1H, 5H-pyridine), 6.79-6.82 (d, 2H, Ar-H), 7.19-7.37 (m, 4H, Ar-H), 7.67-7.70 (d, 1H, Ar-H), 8.09 (brs, 1H, NH; cancelled by D2O), 8.23 (s, 1H, CH-pyrimidine), 8.55-8.58 (d, 1H, Ar-H) ppm.HH. MS m/z 476 (M+, 46), 369 (100). Anal. Calcd for C25H24N4O4S: C, 63.01; H, 5.08; N, 11.76. Found: C, 63.04; H, 5.10; N, 11.74.


2.7.3. Ethyl 3-(2-aminoethyl)-4-imino-5-(4-methoxyphenyl)-4,5-dihydro-3H-benzo-[4',5']thiazolo[3',2':1,6]pyrido[2,3-d]pyrimidine-6-carboxylate (10c)

Pal yellow; Mp 188-190°C; FTIR (KBr, cm-1): 3426 (br, NH2, NH), 1671 (CO). 1H NMR (DMSO-d6, 300 MHz): 1.25 (t, 3H, CH3CH2), 2.08-2.09 (m, 4H, N-CH2CH2-N), 3.67 (s, 3H, OCH3), 4.10-4.18 (m, 4H, CH3CH2 + NH2; cancelled by D2O), 5.10 (s, 1H, 5H-pyridine), 6.79-6.82 (d, 2H, Ar-H), 7.19 (t, 1H, Ar-H), 7.24-7.29 (m, 3H, Ar-H), 7.64-7.66 (d, 1H, Ar-H), 7.85 (brs, 1H, NH; cancelled by D2O),8.11 (s, 1H, CH-pyrimidine), 8.41-8.44 (d, 1H, Ar-H) ppm. MS m/z 475 (M+, 33), 368 (100). Anal. Calcd for C25H25N5O3S: C, 63.14; H, 5.30; N, 14.73. Found: C, 63.12; H, 5.32; N, 14.76.


2.7.4. Ethyl 3-(3-aminopropyl)-4-imino-5-(4-methoxyphenyl)-4,5-dihydro-3H-benzo-[4',5']thiazolo[3',2':1,6]pyrido[2,3-d]pyrimidine-6-carboxylate (10d)

Pal yellow; Mp 210-212°C; FTIR (KBr, cm-1): 3433 (br, NH2, NH), 1661 (CO). 1H NMR (DMSO-d6, 300 MHz): 1.28 (t, 3H, CH3CH2), 1.89 (m, 2H, N-CH2CH2CH2-NH2), 3.18 (t, 2H, N-CH2CH2CH2-NH2), 3.61 (t, 2H, N-CH2CH2CH2-NH2), 3.69 (s, 3H, OCH3), 4.05-4.18 (m, 4H, CH3CH2 + NH2; cancelled by D2O), 5.09 (s, 1H, 5H-pyridine), 6.99-6.81 (d, 2H, Ar-H), 7.19-7.36 (m, 4H, Ar-H), 7.56-7.60 (d, 1H, Ar-H). 8.10 (brs, 1H, NH; cancelled by D2O), 8.21 (s, 1H, CH-pyrimidine), 8.62-8.66 (d, 1H, Ar-H) ppm. MS m/z 489 (M+, 41), 382 (100). Anal. Calcd for C26H27N5O3S: C, 63.78; H, 5.56; N, 14.30. Found: C, 63.80; H, 5.57; N, 14.34.

2.8. Synthesis of Compound 11

Mixture of 10a (2.24g, 5 mmol) and benzaldehyde (5 mmol) were refluxed in ethanol in presence of piperidine (0.5 mL) for 4 hrs. The solvent was removed under reduced pressure and the solid formed was collected by filtration, washed by ethanol and crystallized from ethanol/benzene mixture (1:1) to give compound 11.


2.8.1. (E)-ethyl3-(benzylideneamino)-4-imino-5-(4-methoxyphenyl)-4,5-dihydro-3H-benzo[4',5']thiazolo[3',2':1,6]pyrido[2,3-d]pyrimidine-6-carboxylate (11)

Yellow; Mp 213-215°C; FTIR (KBr, cm-1): 3311 (NH), 1666 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.23 (t, 3H, CH3CH2), 3.63 (s, 3H, OCH3), 4.20 (q, 2H, CH3CH2), 5.75 (s, 1H, 5H-pyridine), 6.79-6.83 (d, 2H, Ar-H), 7.31-7.57 (m, 5H, Ar-H + NH), 7.77-7.80 (d, 1H, Ar-H), 8.25 (s, 1H, CH=N), 8.53-8.71 (d, 1H, Ar-H), 9.77 (s, 1H, CH-pyrimidine) ppm. MS m/z 535 (M+, 42), 428 (100). Anal. Calcd for C30H25N5O3S: C, 67.27; H, 4.70; N, 13.08. Found: C, 67.25; H, 4.72; N, 13.11.

2.9. Synthesis of Compounds 12a,b

A solution of 10a (2.24 g, 5 mmol) and ethyl cyanoacetate (0.57 g, 5 mmol) or triethyorthoformate (0.75 g, 5 mmol) in dry ethanol (30 mL) was refluxed for 3 h to give 12a,b.The solids formed were collected by filtration, washed with ethanol and recrystallized from dioxan.


2.9.1. Ethyl 4-(4-methoxyphenyl)-4H-benzo[4',5']thiazolo[3',2':1,6]pyrido[3,2-e]-[1,2,4]triazolo[1,5-c]pyrimidine-5-carboxylate (12a)

Yellow; Mp 275-277°C; FTIR (KBr, cm-1): 1678 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.18 (t, 3H, CH3CH2), 3.63 (s, 3H, OCH3), 4.11 (q, 2H, CH3CH2), 5.66 (s, 1H, 4H-pyridine), 6.74-6.76 (d, 2H, Ar-H), 7.23-7.44 (m 4H, Ar-H), 7.73-7.77 (d, 1H, Ar-H), 8.62-8.64 (d, 1H, Ar-H), 9.75 (s, 1H, triazole-H), 9.77 (s, 1H, pyrimidine-H) ppm. MS m/z 457 (M+, 28), 350 (100). Anal. Calcd for C24H19N5O3S: C, 63.01; H, 4.19; N, 15.31. Found: C, 62.98; H, 4.21; N, 15.29.


2.9.2. Ethyl 2-(cyanomethyl)-4-(4-methoxyphenyl)-4H-benzo[4',5']thiazolo[3',2':1,6]-pyrido[3,2-e][1,2,4]triazolo[1,5-c]pyrimidine-5-carboxylate (12b)

Yellow; Mp 253-255°C; FTIR (KBr, cm-1): 2221 (CN), 1672 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.22 (t, 3H, CH3CH2), 3.63 (s, 3H, OCH3), 4.12-4.17 (q, 2H, CH3CH2), 4.51 (s, 2H, CH2CN), 5.63 (s, 1H, 4H-pyridine), 6.76-6.81 (d, 2H, Ar-H), 7.21-7.43 (m, 4H, Ar-H), 7.75-7.79 (d, 1H, Ar-H), 8.64-8.68 (d, 1H, Ar-H), 9.78 (s, 1H, pyrimidine-H) ppm. MS m/z 496 (M+, 12), 389 (100). Anal. Calcd for C26H20N6O3S: C, 62.89; H, 4.06; N, 16.93. Found: C, 62.91; H, 4.09; N, 16.95.

2.10. Synthesis of Compounds 12c

A mixture of 10a (2.24 g, 5 mmol) and acetyl chloride (0.5 g, 5 mmol) were refluxed in dry benzene for 4 hrs. The solvent was removed under reduced pressure to give a yellowish solid which was collected by filtration, washed with ethanol and crystallized from dioxan to give compound 12c.


2.10.1. Ethyl 4-(4-methoxyphenyl)-2-methyl-4H-benzo[4',5']thiazolo[3',2':1,6]-pyrido[3,2-e][1,2,4]triazolo[1,5-c]pyrimidine-5-carboxylate (12c)

Yellowish; Mp 281-283°C; FTIR (KBr, cm-1): 1679 (CO-ester). 1H NMR (DMSO-d6, 300 MHz): 1.22 (t, 3H, CH3CH2), 2.40 (s, 3H, CH3-triazole), 3.63 (s, 3H, OCH3), 4.13 (q, 2H, CH3CH2), 5.61 (s, 1H, 4H-pyridine), 6.76-6.81 (d, 2H, Ar-H), 7.21-7.41 (m, 4H, Ar-H), 7.72-7.75 (d, 1H, Ar-H), 8.63-8.67 (d, 1H, Ar-H), 9.62 (s, 1H, pyrimidine-H); 13C NMR (75 MHz, DMSO-d6): 14.28, 14.35, 37.16, 54.95, 60.18, 95.08, 97.02, 105.78, 113.91, 117.76, 122.23, 124.26, 126.12, 126.76, 128.30, 136.03, 136.77, 143.35, 152.73, 158.22, 166.27 ppm. MS m/z 471 (M+, 29), 364 (100). Anal. Calcd for C25H21N5O3S: C, 63.68; H, 4.49; N, 14.85. Found: C, 63.70; H, 4.50; N, 14.87.

3. Results and Discussion

Thus reaction of 3 with arylidenemalononitrile (4a-e) in EtOH/TEA solution was performed at room temperature to give ethyl 1-amino-2-cyano-3-(substituedphenyl)-3H-benzo[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (5a-e) Scheme (1).

The chemical structure of compound 5 was established on the basis of its spectral data. The IR of 5a showed (NH2) at 3430, 3337 and (CN) at 2186 while the (CO-ester) resonated at 1668. 1HNMR showed (CH3) at 1.20 and (CH2) at 4.11-4.17 while the 3H-pyridine resonated at 4.61. The (NH2) appeared at 6.49 as a broad singlet. The mass spectroscopy of 5a showed a molecular ion peak at 375.

Also, the 1H NMR for compounds 5b-e showed peaks at 4.63, 4.54, 4.58 and 4.52, respectively; corresponding to 3H-pyridine while the (NH2) group was resonated at 6.53, 6.48, 6.44 and 6.45, respectively.

The 13C NMR of compound 5c showed signals at 15.45, 68.21 corresponding to the (CH3-CH2-ester) while the (OCH3) appeared at 56.18. The 3C-pyridine appeared at 99.71 while the (CN) showed at 117.76. The (CO-ester) resonated at 159.43.

Formation of compound 5 is assumed to proceed via Michael addition of ethyl 2-(benzo[d]thiazol-2-yl)acetate (3) to ylidenic bond in 4 forming an acyclic intermediate, which cyclized by nucleophilic attack of the NH on the cyanocarbon followed by tautomerization to the final product 5 (Scheme 2).

When 3 was reacted with 2-(ethoxymethylene)malononitrile (4f) under the same reaction conditions, ethyl 2-cyano-1-imino-1H-benzo[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (6) was obtained (Scheme 3).

The chemical structure of compound 6 was confirmed on the basis of its spectral data. IR of compound 6 showed the absence of (NH2) and appearance of absorption peak at 3464 for (NH), while (CN) was observed at 2218 and (CO-ester) resonated at 1683. The 1H NMR showed peaks at 1.33 and 4.29 corresponding to the (CH3CH2-ester) while a peak at 8.07 was appeared equivalent to (pyridine-CH=). A broad singlet was also observed at 8.38 corresponding to the (NH) group. The mass spectrum of compound 6 showed a molecular ion at 297.

Formation of compound 6 is also assumed to proceed via Michael addition of ethyl 2-(benzo[d]thiazol-2-yl)acetate (3) to ylidenic bond in 4f followed by formation of an acyclic intermediate, which cyclized by nucleophilic attack of the NH on the cyanocarbon and then followed by elimination of one ethanol molecule to give the final product 6 (Scheme 4).

Interaction of 3 with ethyl 3-(substituted phenyl)-2-cyanoacrylate (7a-c) in MeOH/TEA at room temperature gave, in case of 7a,b, ethyl 2-(amino(methoxy)methyl)-3-(substituted phenyl)-1-oxo-1H-benzo[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (8a,b) while diethyl 1-amino-3-(4-methoxyphenyl)-3H-benzo[4,5]thiazolo[3,2-a]pyridine-2,4-dicarboxylate (8c) was obtained, in case of 7c (Scheme 5).

The chemical structure of these compounds (8a-c) has been elucidated depending on their spectroscopic analysis. IR for compounds 8a,b showed absorption peaks for (NH2) at 3465, 3373 and 3473, 3399 cm-1; respectively. Other peaks have been detected at 1671 corresponding to the (CO-ester) in addition to absorption peak at 1654 and 1657 for (CO-amide). 1H NMR for both 8a,b showed a new signal at 3.66 and 3.65 equivalent to (OCH3) while singlet at 5.10 and 5.08 has been observed corresponding to (-CH-) for the methyl-side chain. The signal corresponding to 3H-pyridine was not detected. The mass spectrum of 8a gave a molecular ion peak at 408. Compound 8c showed signals at 1.20, 1.24 and 4.11, 4.18 corresponding to the (2CH3CH2-ester). The mass spectroscopy showed a molecular ion peak at 452.

The formation of compounds 8a,b could be attributed to the formation of a Michael adduct followed by nucleophilic attack of the NH on the carbonyl-carbon with elimination of ethanol followed by solvolysis of the nitrile group and tautomirization to give the final product 8a,b (Scheme 6).

Unfortunately, attempts to react 5 with different reagents such as acetic anhydride, acetyl chloride, benzoyl chloride, chroroacetyl chloride, aromatic aldehydes and alkyl cyanides under different reaction conditions were unsuccessful.

Treatment of 5c with triethylorthoformate in acetic anhydride at refluxing temperature gave the correspondingethyl 2-cyano-1-((ethoxymethylene)amino)-3-(4-methoxyphenyl)-3H-benzo[4,5]thiazolo[3,2-a]pyridine-4-carboxylate (9) which was treated with different amines to afford the ethyl 3-substituted-4-imino-5-(4-methoxyphenyl)-4,5-dihydro-3H-benzo[4',5']thiazolo[3',2':1,6]pyrido[2,3-d]py-rimidine-6-carboxylate (10a-d) (Scheme 7).

The structures of compound 9 and 10 were established on the basis of IR, 1H NMR, 13C NMR and MS data. The IR spectra of compounds 9 showed the presence of CN stretches at 2202 cm-1 and a peak at 1672 corresponding to the (CO-ester). The 13C NMR showed signals at 14.19 (CH3-ester), 14.21 (CH3-ethoxy), 60.26 (CH2-ester), 64.59 (CH2-ethoxy) and (CH=pyrimidine) at 150.14. The mass spectroscopy showed a molecular ion peak at 461. 1H NMR for compounds 10a-d showed signals at 8.09-8.23 corresponding to (CH=pyrimidine), while the (CH=pyrimidine) was resonated in 13C NMR at 145.62 for 10a.

The imino compound 10a proved to be a useful intermediate for the synthesis of a variety of 2-substituted-4H-benzo[4',5']thiazolo[3',2':1,6]pyrido[3,2-e][1,2,4]triazolo[1,5-c]pyrimidine-5-carboxylat-ederivatives. Thus, treatment of 10awith triethylorthoformate, ethyl cyanoacetate and/or acetyl chloride in different solvents afforded ethyl 4-(4-methoxyphenyl)-4H-benzo[4',5']thiazolo[3',2':1,6]pyrido[3,2-e][1,2,4]triazolo[1,5-c]pyri-midine-5-carboxylate (12a), ethyl 2-(cyanomethyl)-4-(4-methoxyphenyl)-4H-benzo-[4',5']thiazolo[3',2':1,6]pyrido[3,2-e][1,2,4]triazolo[1,5-c]pyrimidine-5-carboxylate (12b) and ethyl 4-(4-methoxyphenyl)-2-methyl-4H-benzo[4',5']thiazolo[3',2':1,6]pyrido[3,2-e][1,2,4]triazolo[1,5-c]pyrimidine-5-carboxylate (12c), respectively (Scheme 8). The 1H NMR spectra of 12 showed signals at 9.62-9.78 corresponding to pyrimidine-H while 12a showed signal at 9.75 corresponding to triazolo-H.

Condensation of 10a with benzaldehyde under reflux in ethanolic piperidine afforded the open chain productethyl 3-(benzylideneamino)-4-imino-5-(4-methoxy-phenyl)-4,5-dihydro-3H-benzo[4',5']thiazolo[3',2':1,6]pyrido[2,3-d]pyramidine-6-carbox-ylate (11) (Scheme 8). The structure of 11 was established on the basis of its IR spectrum, which showed the presence of NH absorption at 3311 cm-1. The 1H NMR spectra of 11 showed signals at 8.25 (s, 1H, N=CH) and 9.77 (s, 1H, pyrimidine-H).

4. Conclusion

Ethyl 1-amino-3-(substituted phenyl)-2-cyano-3h-benzo[4,5]thiazolo-[3,2-a]pyridine-4-carboxylate derivatives (5a-e) were prepared by convenient method. Reaction of ethyl 2-(benzo[d]thiazol-2-yl)acetate (3) with ylidenic compounds resulted in formation of pyridine derivatives (6, 8a-c). When compound 5c was reacted with triethylorthoformate, the ethoxymethyleneaminopyridine-4-carboxylate derivative (9) was obtained. The triazolo[1,5-c]pyrimidine-5-carboxylate derivatives (12a-c) were obtained when 10a was reacted with electrophilic reagents.

References

[1]  D. Shi, T. Bradshaw, S. Wrigley, C. McCall, P. Lalieveld, I. Fichhtner and M. Stevens, J. Med. Chem, 39, 3375, 1996.
In article      CrossRef
 
[2]  S. Choi, H. Park, S. Lee, S. Kim, G. Han and H. Choo, Bioorg.& Med. Chem., 14, 1229, 2006.
In article      CrossRef
 
[3]  H. A. Bhuva, S. G. Kini, J. Mole. Graph. Mod., 29, 32, 2010.
In article      CrossRef
 
[4]  C. G. Mortimer, G. Wells, J. P. Crochard, E. L. Stone, T. D. Bradshaw, M. F. G. Stevens and A. D. West well, J. Med. Chem, 49, 179, 2006.
In article      CrossRef
 
[5]  G. Alang, R. Kaur, A. Singh, P. Budhlakoti, A. Singh and R. Sanwal, Inter. J. Pharma. & Biolo. Arch., 1(1), 56, 2010.
In article      
 
[6]  S. H. Suresh, R. J. Venkateshwara, K. N. Jayaveera, Res. J. Pharma., Biolo.Chem. Sci., 1(4), 635, 2010.
In article      
 
[7]  A. Yadav, P. Sharma, V. ranjeeta, S. Sunder and U. Nagaich, The Pharma Res., 1, 182, 2009.
In article      
 
[8]  K. M. Basavaraja, B. Somashekhar and B. Shivakumar, Inter. J. Pharm Tech Res., 2(2), 1139, 2010.
In article      
 
[9]  M. Maharan, S. William, F. Ramzy and A. Sembel, Molecules, 12, 622, 2007.
In article      CrossRef
 
[10]  P. Venkatesh and S. N. Pandeya, Inter. J. Chem Tech Res., 1(4), 1354, 2009.
In article      
 
[11]  D. Shashank, T. Vishawanth, M. A. Prasha, V. Balasubramaniam, A. Nagendra, P. Perumal and R. Suthakaran, Inter. J. Chem Tech Res., 1(4), 1224, 2009.
In article      
 
[12]  H. Kaur, S. Kumar, I. Singh, K. K. Saxena and A. Kumar, Dig. J. Nanomat. Biostru., 5(1), 67, 2010.
In article      
 
[13]  V. Kumar, T. S. Ngaraja, H. Shameer, E. Jayachandran and G. M. Sreenivasa, J. Pharma. Sci. Res., 2, 83, 2009.
In article      
 
[14]  N. Siddiqui, S. N. Pandeya, S. Khan, J. Stables, A. Rana, M. Alam, M. Arshad and M. Bhat, Bioorg.& Med. Chem. Lett., 17, 255, 2007.
In article      CrossRef
 
[15]  N. Amnerkar and K. P. Bhusari, Euro .J. Med. Chem., 45, 149, 2010.
In article      CrossRef
 
[16]  S. R. Pattan, Ch. Suresh, V. D. Pujar, V. V. K. Reddy, V. P. Rasal and B. C. Koti, Ind. J. Chem., 44B, 2404, 2005.
In article      
 
[17]  C. Micael, V. Zandt, M. Jones, D. Gunn, L. Geraci, J. Jones, D. SAwicki, J. Sredy, J. Jacot, A. T. DiCioccio, T. Petrova, A. Mitschler and A. D. Podjarny, J. Med. Chem., 48, 3141, 2005.
In article      CrossRef
 
[18]  P. Arora, S. Das, M. S. Ranawat, N. Arora and M. M. Gupta, J. Chem. and Pharm. Res., 2(4), 317, 2010.
In article      
 
[19]  M. S. S.Yar and Z. H. Ansari, Act. Polon. Pharm.-Drug Res., 66(4), 387, 2009.
In article      
 
[20]  S. Rostamizadeh and S. A. G. Housaini, Phosphorus, Sulfur, and Silicon, 180, 1321, 2005.
In article      CrossRef
 
[21]  S. S. Patil and V. D. Bobade, Synt. comm., 40, 206, 2010.
In article      CrossRef
 
[22]  V. P. DevmurariandT. J. Ghodasara, Arch. App. Sci. Res., 2(1), 198, 2010.
In article      
 
[23]  D. L. Boger, J. Org. Chem., 43, 2296, 1978.
In article      CrossRef
 
[24]  R. H. Tale, Org. Lett., 4(10), 1641, 2002.
In article      CrossRef
 
[25]  G. EvindarandR. A. Batey, J. Org. Chem., 71, 1802, 2006.
In article      CrossRef
 
[26]  V. Rey, S. Castro, J. Arguello and A. Penenory, Tetra. Lett., 50, 4720, 2009.
In article      CrossRef
 
[27]  A. M. El-Agrody, F. A. Eid, H. A. Emam, H. M. Mohamed and A. H. Bedair,Z. Naturforsch. Teil B, 57, 579, 2002.
In article      
 
[28]  F. A. Eid, A. H. Bedair, H. A. Emam, H. M. Mohamed and A. M. El-Agrody,Al-Azhar Bull. Sci., 14, 311, 2003.
In article      
 
[29]  A. S. Abd-El-Aziz, H. M. Mohamed, S. Z. S. Mohammed, A. Ata, A. H. Bedair, A. M. El-Agrody and P. D. Harvey, J. Heterocycl. chem., 44, 1287, 2007.
In article      CrossRef
 
[30]  N. M. Sabry, H. M. Mohamed, E. Sh. Khattab, S. S. Motlaq and A. M. El-Agrody, Eur. J. Med. Chem., 46, 765, 2011.
In article      CrossRef
 
[31]  A. H. F. Abd El-wahab, H. M. Mohamed, A. M. El-Agrody, A. H. Bedair, F. A. Eid, M. M. Khafagy and K. A. Abd-El-Rehem, Amer. J. Chem., 1(1), 1, 2011.
In article      
 
[32]  H. M. Mohamed, A. H. F. Abd El-Wahab, K. A. Ahmed,A. M. El-Agrody, A. H. Bedair, F. A. Eid and M. M. Khafagy, Molecules, 17, 971, 2012.
In article      CrossRef
 
[33]  A. H. F. A. El-Wahab, H. M. Mohamed, A. M. El-Agrody, M. A. El-Nassag and A. H. Bedair, Eur. J. Chem., 4(1), 10, 2013.
In article      CrossRef
 
[34]  H. M. Mohamed, I. A. Radini, A. M. El-Agrody and A. M. Al-Ghamdi, LDDD, 10, 758, 2013.
In article      CrossRef
 
[35]  A.Abbotto, S.Bradamante, A.Facchetti, and G. A. Pagani, J. Org. Chem., 67, 5753, 2002.
In article      CrossRef
 
comments powered by Disqus
  • CiteULikeCiteULike
  • MendeleyMendeley
  • StumbleUponStumbleUpon
  • Add to DeliciousDelicious
  • FacebookFacebook
  • TwitterTwitter
  • LinkedInLinkedIn