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Figure 3. Treatment of the image of chromatogram spots with the software ImageJ. From top to bottom: first layer – digitalized chromatogram spots; second layer – black-and-white converted images of the spots; third layer – outlines of the spots; fourth layer – areas of the spots, as determined by the software

World Journal of Chemical Education. 2017, 5(5), 148-152 doi:10.12691/wjce-5-5-1