Figure 3. Treatment of the image of chromatogram spots with the software ImageJ. From top to bottom: first layer – digitalized chromatogram spots; second layer – black-and-white converted images of the spots; third layer – outlines of the spots; fourth layer – areas of the spots, as determined by the software

From

TLC Procedure for Determination of Approximate Contents of Caffeine in Food and Beverages

Carmen Palacios, Maria L. F. Salatino, Antonio Salatino

World Journal of Chemical Education. 2017, 5(5), 148-152 doi:10.12691/wjce-5-5-1