Seed Potato Production Practices and Quality of Farm Saved Seed Potato in Kiambu and Nyandarua Counties in Kenya

Potato production in Kenya is mainly constrained by limited supply of quality seed potato tubers. The objective of this study was to determine seed potato handling practices and quality of farm saved seed potato. A survey involving 79 farmers was conducted in potato production areas in Kiambu and Nyandarua Counties to collect information on seed potato production practices including sources of seed tubers, seed selection, seed tuber storage, pests and diseases. Samples of seed potato were collected from farmers and evaluated for quality parameters such as tuber size, weight, stout sprout length and infection with diseases. Factors affecting potato production included limited quality seed supply, pests and diseases. Shangi was the main potato variety grown by majority (62.8%) of farmers and all the farmers used own farm saved seed. Most (45.5%) farmers recycled the seed for four seasons and 44.1% of farmers stored seed potato for three months. Freeness from seedborne diseases was the main seed quality preference by farmers. The main pest reported was cutworms (42.6% of farmers) while potato late blight and bacterial wilt were the major diseases reported by 87.1% and 86.7% of the farmers respectively. All farm saved seed potato samples were infected with Fusarium coeruleum, 51.2% with Rhizoctonia solani, 53.3% with Fusarium sambunicum, 33.3% with Fusarium solani and 48.3% with Aspergillus niger. Over 69% of the farm saved seed samples were infected with Ralstonia solanacearum while 40.1% were infected with Potato Virus S, the most dominant. Due to poor post-harvest handling practices, farmers incurred seed quality and quantity losses in storage. Farm saved seed is contaminated with multiple seed borne diseases. Farmers should be sensitized on appropriate seed potato handling practices and there should be increased supply of certified seed potato.


Introduction
Potato is the second most important food crop in Kenya after maize and has been used to address food security challenges with declining yield in cereals [1,2]. Due to limited supply of certified seed potato, the main challenge facing potato production in Kenya is use of poor quality seed potato tubers that are highly contaminated with seed borne diseases [3,4]. The informal seed potato supply system made up of farm saved, seed exchanges by farmers and local markets is the main channel of acquiring seed potato tubers in developing countries and it accounts for over95% of seed potato volumes used [5,6,7,8]. Studies in Kenya and Uganda have shown that over 70% of farmers practice variety and seed selection at farm level in potato production [9]. In Kenya, over 95% of potato farmers use farm saved seed tubers which are of poor quality especially with accumulation of tuber borne diseases [5,7]. Seedborne diseases such as bacterial wilt (Ralstonia solanacearum), late blight of potato (Phytopthora infestans), dry rot (Fusarium spp.) and potato viruses are carried across cropping seasons leading to an overall decline in seed quality and causing high yield losses [10,11,12].
The main intervention mechanism to averting yield losses is promotion of the use of quality seed potato that is disease free but lack of adequate supply of certified seed potato is a major constraint to farmers [3,6,13]. Therefore, most farmers do not use certified seed due to limited supply, high prices and lack of knowledge on its importance [3,9]. There is no quality control in seed potato production from informal seed systems and the tubers are of low phytosanitary status [14,15]. Seed potato quality attributes include seed health, physical and genetic purity, seed tuber size and the physiological age of tubers [6]. These attributes are met through seed certification in formal seed production unlike the informal seed system where there is no quality control [14] . Seed health is the World Journal of Agricultural Research 21 most important attribute since seed borne diseases cause the highest losses [4,16,17]. Due to limited supply of certified seed, most farmers recycle seed tubers for many seasons causing an overall decline in seed quality due to accumulation of seed borne diseases such as potato viruses through seed degeneration [11].
Quality attributes used in seed selection include yielding ability, genetic purity, disease resistance, days to maturity, marketability, and size of tubers [6,9]. Lack of adequate knowledge on recommended potato production practices is a major problem facing farmers [18,19]. To mitigate the problems associated with limited supply of certified seed potato, positive selection technique was developed to enhance production of quality seed at the farm level but few farmers have adopted this technology [18,20]. Farmers' knowledge on potato diseases, pests and their management is important in potato production [19]. Information flow in potato production affects choices on seed acquisition and production practices. Information sources such as neighbours, markets, Non-Governmental Organizations (NGOs), extension officers and researchers on seed sources and production practices influences the yield obtained by farmers [5,6]. In addition, sources of seed potato, production and post-harvest handling practices affects quality of seed tubers [21].
This study therefore aimed at determining seed potato production practices and quality of farm saved seed potato among small scale farmers in Kiambu and Nyandarua Counties in Kenya.

Determination of Seed Potato Production and Handling Practices
This study was conducted in four Agro-Ecological Zones (AEZs) in Kiambu and Nyandarua Counties in Kenya. The AEZs in Kiambu County were Lower Highland Zone II (LH2) and Upper Midland Zone I (UMI). In Nyandarua County, the AEZs were Upper Highland Zone II (UH2) and Upper Highland Zone III (UH3) [22]. A survey involving 79 farmers was conducted using a questionnaire and the sample size was determined using the formula shown below: Where n is the sample size, N is the population size. The assumptions of using the formula; There is 95% confidence level, P= 0.5 and error limit (e) = 0.1 [23]. Information collected included duration of potato production, varieties grown, sources of seed potato, quality traits used in seed selection, duration before seed renewal, methods of seed storage, pests and diseases. Farmers were interviewed in potato growing groups where 15 farmers were interviewed in LH2, 16 in UM1, 25 in UH2 and 23 in UH3. Two kilograms of farm saved seed tubers were obtained from each farmer and taken for seed quality analysis at the Faculty of Agriculture, University of Nairobi.

Determination of Physical and Physiological Quality
Determination of physical quality involved measurement of seed tuber size and tuber weight while physiological quality involved determination of number of sprouts and sprout length following the United Nations Economic Commission for Europe (UNECE) and Food Agriculture Organization (FAO) standards on Quality Control in Seed Potatoes [24,25]. Seed potato tubers sizes were graded on basis of four diameter scales namely chatts (<20mm diameter), small (20mm-25mm), medium (25-35mm) and large (30-35mm). The weight of individual tubers regardless of the size was taken using a digital balance. Sprouted and unsprouted eyes per tuber were also counted regardless of size. Length of stout sprouts per tuber was measured using a string and a ruler. Data was collected on the number of tubers in various sizes, weight of individual tubers, sprouted eyes and stout sprout length.

Determination of Tuber-borne Infection
Fungal, bacterial and viral diseases were detected in determination of seed borne diseases in farm seed tubers. Detection of dry rot (Fusarium spp.) and black scurf (Rhizoctonia solani) was done using methods described by [26] and [27] respectively. Three seed tubers per sample were randomly drawn and washed under running tap water. Three slices each 6mm by 5mm were excised from each tuber using a sterilized scalpel. The slices were sterilized in 1% NaOCl solution for three minutes and rinsed in three changes of distilled water. The slices were plated on molten Potato Dextrose Agar (PDA) medium at equidistant positions and incubated for seven days at room temperature (23±2°C). Resultant colonies were sub cultured on PDA and pure cultures identified using colony morphological characteristics under light microscope. Data was collected on the number of infected slices with fungal pathogens and the individual fungal colony types.
An Enzyme Linked Immunosorbent Assay on Nitrocellulose Membrane (NCM-ELISA) method described by [28] using a kit sourced from International Potato Center (CIP), Lima Peru was used to detect bacterial wilt (Ralstonia solanacearum) in farm saved seed tubers. Three tubers from each replicate of each sample were tested. The tubers were washed in tap water and dipped in 1% NaOCl for one minute. The stolon end of each tuber was cut transversally, vascular ring scooped out and crushed in a crushing buffer. The extract was enriched overnight by adding 500μl of enrichment broth. The extracts were loaded alongside positive and negative controls on nitrocellulose membranes soaked in a buffer solution and allowed to dry. Blocking solution was added and allowed to react for an hour with continuous agitation. Binding of R. solanacearum antibodies were done by soaking the membranes in an antibody solution for two hours with continuous agitation. Membranes were soaked in a solution with conjugated antibodies for an hour and washed three times using a washing buffer. Colour development was done by adding colour development solution and reaction allowed to run for 30 minutes with formation of purple colour in samples similar to the 22 World Journal of Agricultural Research positive controls. Data was collected on samples testing positive for the disease as outlined in the kit [28].
Detection of potato viruses was done using Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) according to [29,30]. The DAS ELISA kit was also sourced from CIP, Peru and can detect six potato viruses namely Potato Leaf Roll Virus (PLRV), Potato Virus A (PVA), Potato Virus M (PVM), Potato Virus S (PVS), Potato Virus X (PVX) and Potato Virus Y (PVY). The tubers were allowed to sprout before detection for virus infection. For each virus, 20µl of antibody were dissolved in 20µl of coating buffer, loaded on microtiter plates and incubated for four hours. The leaf and sprout samples were crushed in a crushing buffer and the extract loaded on washed coated plates. Three healthy controls specific to each virus were filled in the last three wells of each plate and loaded plates were incubated overnight. Plates were washed thrice in a washing buffer and conjugate solution added followed by incubation for five hours. Washing was done thrice and substrate solution added until development of yellow colour with healthy controls remaining clear. The number of samples positive for viruses was determined using the concentration of the virus titre calculated using the formula:

Statistical Data Analysis
The survey data was analyzed using IBM SPSS© statistical software version 20. Bacterial wilt infection and viruses were interpreted by comparing the samples with the positive controls as outlined in the kits. Percentage disease infection after ELISA tests, seed tuber weight and sprouting subjected to analysis of variance using GENSTAT© statistical package. Mean separation done using Fisher's protected Least Significant Difference at 5% level of significance [31].

Seed Potato Production and Handling Practices
Most (58.5%) famers grew potato for over five years, only 2.5% grew for one and two years and none grew the crop for just one season (Table 1). Shangi (62.8%) was the main variety grown by 62.8% of farmers compared to Kenya Karibu and Dutch Robjin which was the least grown (Table 2).  Majority (45.5%) of the farmers took four seasons to renew seed potato, while 17.2% and 11.4% renewed after six and three seasons respectively. Only 1.7% renewed seed after one season while16.1% did not renew seed potato tubers at all (Table 3). Farm saved seed potato was used by all farmers. Local markets (72.8%), neighbours (31.2%) and certified seed from research institutions (18.3%) were other alternative seed sources. Local markets were the second most important seed source and very few farmers used certified seed (Table 4). Freeness from seed borne diseases (69.6%) was the main seed quality preference, seed tuber size (24.8%) and sprouting capacity (5.4%) were the additional seed quality attributes (Figure1). Polythene and jute bags were the main (46%) methods of seed tuber storage, farm stores (37.7%), diffused light storage (5.7%), heaps (4.4%), dark storage (3.2%) and field pits (3%) were also used as methods of storing seed tubers (Table 5).    Majority (44.1%) of the farmers stored seed potato for three months but two (33.8%), four (4.8%) and one month (4%) were other storage periods (Table 6). Pests (69.7%) were the main storage constraint followed by seed tuber rot (17.8%), poor sprouting (12.1%) and weight loss (0.45%). Very few (0.45%) farmers reported weight loss as a storage constraint (Table 7). Cutworms (42.6%) were the major field pest unlike potato tuber moth (38.8%) the main storage pest. Rodents (20%) and whiteflies (1.5%) were also reported ( Table 8). Potato late blight (87.1%) and bacterial wilt (86.7%) were the major potato diseases faced by farmers in potato production ( Figure 2).

Physical Quality and Infection with Tuber-Borne Disease Pathogens
There were no significant differences in small and medium sized tubers used by farmers. However, there were slight variations in medium and large tubers ( Table 9). The length of sprouts varied significantly across the sites unlike the weight of seed tubers which were not significantly different at P ≤0.05 (Table 9). There was up to 87% R. solanacearum infection in farm saved tubers. Despite high R. solanacearum infection levels in farm saved seed potato across the four agro-ecological zones, there were no significant differences on disease infection in samples collected from LH2and UM1 unlike significance differences observed in samples collected from UH3 and UH2. Only UH3 had significantly least proportion of samples infected with bacterial wilt with up to 69% of samples testing positive for the disease. There was higher bacterial wilt infection in Kikuyu (LH2 and UM1) compared to Nyandarua (UH2 and UH3, (Table 9). There were significant differences among farm saved seed samples in infection with seedborne fungal pathogens in the four agro-ecological zones. All the farm saved seed samples were infected with Fusarium coeruleum which was the most prevalent pathogen and was significantly different unlike Fusarium solani the least. Within the agro-ecological zones, there were significant differences in infection with all fungal pathogens in LH2, UH2 and UH3 unlike in UM1 where only F. coeruleum significantly different. Fusarium coeruleum was the most prevalent pathogen across the four sites with at least 93.3% of the samples infected. Aspergillus niger was the least fungal pathogen found to infect farm saved tubers in LH2 and UM1 unlike Fusarium sambunicum in UH2 and Fusarium solani in UH3. Highest prevalence with fungal pathogens was in UH2 and UH3 (Table 10).  There was a significant variation in the mean samples infected with potato viruses and PVS was the post prevalent with up to an average of 40.2% of the samples were infected. Potato Virus A (PVA) and PVM were not found to infect farm saved seed potato tubers after DAS-ELISA detection. Within the agro-ecological zones, PVS was still the most infectious and significantly different in all the AEZs. In UM1, both PLRV and PVX were not significantly different unlike in UH2 where there were significant differences in the viruses detected with PVS being the most prevalent compared PVX. In UH3, PVS and PVY were significantly different and they were the only viruses detected. There was higher virus infection in samples obtained from Nyandarua compared to Kikuyu (Table 11).

Seed potato production practices by small-scale farmers
The results showed that majority of the farmers had been in potato production for over three years. There is increased profitability of potato since the crop can be grown all year unlike maize which takes up to ten months to mature with only one crop per year in high potential areas. In addition, the sampled agro-ecological zones have low temperatures limiting growth of cereals such as maize hence potato is a dominant crop in these areas due to favorable climate [22]. Some farmers had been involved in potato production for a short duration suggesting diversification of crops from subsistence to market based farming as they increasingly took up production of the crop [10,32]. Studies by [4] revealed high profits accrued from potato production for those farmers who have access to certified seed potato and shifted potato production from subsistence to agribusiness. Longer duration of involvement in potato production could be attributed to higher yields of potato compared to cereals in a limited land area in these agro-ecologies [8]. There has been increasing expansion in land area under potato production in Kenya over the years due to increased profitability of the crop for food security [8,9].
Potato varieties grown included those officially released such as Tigoni and Kenya Mpya and local landraces. Studies by [9] revealed growth of mixed potato varieties by farmers mainly Tigoni and local landraces in Kenya and Uganda. In addition, there has been reported increased uptake of improved potato varieties from public research institutions and NGOs mainly International Potato Center by farmers in Africa [33]. Studies by [8] also revealed increased cultivation of Kenya Karibu, Dutch Robjin and Tigoni potato varieties in Kenya as reported in this study. Shangi was the main variety grown due to its reported acceptability with farmers before official release since National Performance Trials (NPT) are carried out in farmers' fields who access the materials before official release. Some of quality attributes used by farmers in variety selection include marketability, maturity and disease resistance [6]. Recent research has focused on disease resistance and performance of varieties under climate change as opposed to marketability and cooking quality hence farmer preferences may not be met by officially released varieties. Apart from subsistence farming of potato, the ware potato industry in Kenya is also for the fast food industry and the variety Shangi has good peeling, frying and non crumbling quality when processed into fries hence the variety is highly sought by brokers who link farmers with the market. Local landraces were grown for germplasm conservation [9,10]. In addition, [33] reported that due to the need to conserve traditional germplasm, few farmers took newly released and improved potato varieties and continued with local landraces hence this could explain use of traditional varieties by some farmers in this study.
Results in this study showed that most farmers renewed seed after four to six cropping seasons while some did not renew seed at all. These results are concurrent with findings of [33] and [5] both reported that most potato farmers in Eastern Africa renewed seed potato after six to eight seasons. Findings of [5] showed that over 74%, 59% and 56% of farmers in Kenya, Uganda and Ethiopia respectively did not renew seed potato until after six seasons. However, these results are contrary to [9] who reported that only 41% of farmers in Kenya and 25% in Uganda renewed seed potato unlike 83.9% reported in this study. Periodic renewal of seed potato by farmers from informal sources mainly neighbours and local markets have been also reported in previous studies [6,10]. Due to ease of access, most farmers in Kenya use informal channels in seed renewal and with limited availability of certified seed, few farmers in Kenya renew seed [18]. Growth of local landraces for over 35 years without clean up for diseases also suggests no seed renewal by farmers [9]. Due to reasons such as limited supply of certified seed potato, high certified seed prices and challenges in large scale multiplication of certified seed potato which limits supply, farmers resort to recycling seed from the previous harvest across seasons [5,9]. Recycled seed tubers are highly contaminated with seed borne diseases such as bacterial wilt, potato late blight and Fusarium dry that can lead to total yield loss. Seed renewal is important since continuous use of same seed leads to accumulation of seed borne diseases causing persistent disease outbreaks and low yields [11,34].
Multiple channels were used in acquiring seed potato including both informal and formal seed supply systems. The informal system was the main channel used which included own farm saved seed, local markets and neighbours. All the farmers used own farm saved seed tubers while few accessed certified seed potato from research institutions. These results corresponds to the findings by [4,5] who reported that the informal seed system is the main means of seed acquisition in Eastern Africa and over 98% of the farmers used seed potato either from either own fields, neighbours and local markets. In addition, [18] and [35] reported that less than 5% of farmers in Eastern Africa used certified seed potato. Reasons for low usage of certified seed were attributed to inadequate and poor supply, high certified seed prices, inadequate availability of varieties preferred by farmers and lack of knowledge on importance of use of quality seed potato [9,35]. The bulkiness of seed potato being vegetatively propagated and centralized production are among key factors limiting adequate seed supply in Kenya forcing farmers to use informal seed supply means [36]. Limited supply of certified seed potato forces farmers to use informal means in seed acquisition which supplies seed tubers that are of poor quality with high prevalence of seed borne diseases hence low yields [4].
All farmers practiced seed selection and freeness from diseases, size of tubers and the sprouting capacity were the quality preferences. Similar observations were made by [13] by reporting that the main concern for farmers in developing countries is supply of quality seed potato that is free from seed borne diseases especially bacterial wilt (Ralstonia solanacearum) and potato late blight (Phytophthora infestans). However, [9] reported that most farmers in Kenya and Uganda considered yielding ability in variety and seed selection followed by disease resistance. Preference for healthy seed tubers by most farmers was probably because major potato diseases are seed borne attacking the crop in the field and also cause high seed losses during storage [3,4]. In addition, [37] reported that late blight pathogen causes tuber blight, a major storage disease whose inocula remains viable both in storage and in soil hence difficult to control [3]. Farmers use visual selection at harvest and may select latently infected seed potato tubers. Potato production in Kenya is for the ware market and unmarketable (small and chatts) tubers are used as seed hence seed tuber size was reported as a quality trait in this study [38].
Farmers used unspecialized storage methods like jute bags, polythene, heaps, field pits, dark and farm stores while few used diffused light stores leading to high storage losses. Studies by [5] and [9] revealed that most farmers in East Africa stored own seed potato at farm level and did not use recommended storage structures such as diffused light storage and cold stores. In addition, [6] reported that only 5% of farmers in North Western Ethiopia used diffused light stores and storage structures affected seed tuber quality and its storability. Dark and lit spaces in the houses, delayed harvest and field pits are among the alternative methods of seed potato storage reported in Eastern Africa [5]. Use of poor storage structures leads to high seed losses and disease spread in storage resulting from poor storage conditions and improper post-harvest handling practices [39,40,41,42]. In addition, poor seed tuber storage leads to deterioration in quality and quality of the seed due to attack by diseases mainly Fusarium dry rot [42]. Sub-optimal storage environment especially low relative humidity and high temperature leads to increased respiration and transpiration with subsequent loss in food reserves and water respectively. This leads to reduced tuber weight which reduces vigour by depletion of stored food reserves causing poor crop establishment [40].
Most farmers stored potato for two and three months and few stored for one and four months. These results are contrary to [5] who reported that many farmers in Kenya stored seed potato for one to two months unlike three to four months in Uganda and up to six months storage period in Ethiopia. However, [9] reported that many farmers in Kenya stored seed potato for two months unlike three months in Uganda. Short storage period in Kenya is possibly due to potato being a high value crop, shorter storage durations are used allowing production all year round. Shangi was the commonest variety grown and has a short dormancy period lasting one month hence short storage period [43]. A shorter storage period of three weeks in central Kenya for immediate planting has also been reported [9]. Duration of storage is important since shorter storage necessitates artificial breakage of dormancy while longer seed storage periods increases the physiological age of tubers reducing vigour in addition to predisposing the seed to pest and disease attack especially in poor storage structures [6,15].
Pests were the main storage constraint but additional problems faced during seed potato storage included rotting of seed potato tubers, poor sprouting and weight loss. This is in agreement with [15] and [19] who reported potato tuber moth and seed rot as major storage problems in Ethiopia and Uganda respectively arising from use of non-specialized storage structures. In addition, [5] also reported poor sprouting in seed potato stored under poor storage conditions leading to production of poor and elongated sprouts since the type of storage structures have significant effects on sprouting [44]. Use of poor storage structures was the main factor causing storage problems resulting from poor storage hygiene, tuber damage and poor post-harvest handling practices [15,39]. Up to 30% seed damage has been reported in poor structures [41]. Weight loss was reported due to deformation of tubers involving loss in dry matter through respiration and water loss through transpiration which are normal physiological processes affected by the storage environment [40].
The study revealed cutworms as the most damaging pest followed potato tuber moth. Cutworms and rodents were the major field pest while potato tuber moth was the main storage pest. Similar findings were reported by [19] revealing insect pests as the second most important problem after diseases in seed potato storage. However, [45] reported that potato tuber moth to be the most lethal pest unlike cutworms as reported in this study. In addition, [46] reported potato aphids as the most lethal field pests especially in the spread of viral diseases. Cutworms were the most damaging field pest since they cause damage at planting leading to poor field establishment [19]. Pest attack leads to poor crop emergence and low plant stand count thus lowering yields [19]. Storage pests such as potato tuber moth can lead to reduction in marketability of the harvested produce, seed and yield losses [15,45].
Potato diseases namely bacterial wilt (Ralstonia solanacearum) and potato late blight (Phytopthora infestans) were reported to affect potato production. Occurrence of late blight was higher compared to that of bacterial wilt. Studies by [9] and [10] revealed bacterial wilt as the major disease leading to yield reduction in all potato growing areas in Kenya. In addition, [35] and [38] reported bacterial wilt and potato late blight occurrence to be over 70% and 67% respectively in Kenyan farms leading to high yield losses. In addition, [5] reported a comparatively lower (74%) prevalence of bacterial wilt in Kenya than reported on this study. However, [3] reported late blight to be the most damaging potato disease in Kenya. High prevalence of these two diseases could be due to use of poor quality seed tubers from informal seed systems with high infection levels with seed borne diseases [6,47]. High prevalence of bacterial wilt disease across the four study sites can be attributed to poor farming practices and use of contaminated farm saved seed. Studies by [47] attributed the spread of bacterial wilt to be due to continuous monocropping, contaminated runoff water and tools which are all common practices among small scale-farmers potato farmers in Kenya. Increased prevalence of late blight is due to use of infected seed tubers from informal sources and the disease can cause total yield loss [3].

Physical Quality and Infection with Tuber-borne Disease Pathogens
The study showed no significant differences in the sizes of seed potato tubers used by farmers, which did not meet the minimum physical quality standards for seed size [24]. Majority of the farmers were using chatts and small tubers as seed as opposed to recommended medium sized tubers. Seed potato standards from [24,48] do not classify chatts and small tubers as seed but they are used by farmers who select poor and unmarketable tubers from the crop at harvest and convert it to seed after storage [8]. Similar findings were reported by [9,18] who revealed use of chatts as seed tubers by farmers in East Africa due to limited supply of certified seed. Chatts have low weight with poor vigour due to low food reserves since the recommended weight of (medium) tubers ideal for seed is 40-70g [49]. In addition, chatts have high virus infection levels and leads to production of small and misshaped tubers of low marketability [18,34]. The sprout length met the recommended length but the weights (<40g) were below the recommended standard weight range (40-70g) for seed potato tubers [49]. High sprout length could be due to sprout initiation mechanisms used by farmers such as partial exposure to light, use of field pits lined with straw or grass and seed treatments [9,39]. Previous studies have shown significant effect of postharvest handling practices on sprouting of seed tubers eventually affecting the quality of seed hence use of poor storage structures lowers quality of stored seed tubers [44].
The study revealed no significant difference in the weights of seed tubers between the agro-ecological zones because most farmers were using the same size of the seed tubers. Studies by [11] revealed seed higher cases of seed degeneration in areas where chatts and small tubers were used as seed. Use of chatts and small tubers explains the low seed weight or could be due to water and dry matter loss from tubers due transpiration and respiration respectively which is high in poor storage structures [40]. Seed tubers of low weight and poor sprouts have low vigour and poor crop emergence leading to reduced yields [49,50].
Results showed significantly high infection levels with bacterial wilt in all the agro-ecological. These results correspond with [50] who reported 80.7% occurrence of the disease Ethiopia by testing symptomatic samples. In addition, [51] reported up to 99% occurrence of bacterial wilt in Kenya through a survey. In contrast, [5] reported 74% bacterial wilt incidence in Kenya. In addition, [47] reported 70% incidence of bacterial wilt in Kenya causing over 50% yield loss. Use of seed potato from informal supply systems has proliferated spread of the disease. The R. solanacearum inocula is spread through infected soil, volunteer crops, infected seed tubers, runoff water and tools [10]. Studies by [18] revealed high prevalence of brown rot in Eastern Africa to be due to use of seed potato from informal supply such as farm saved as shown in this study. Tubers infected with bacterial wilt have poor crop performance leading high losses [42,53].
There were no significant differences among the agoecological zones on infection with fungal pathogens. Multiple seed borne fungal pathogens were found to infect World Journal of Agricultural Research farm saved seed potato and Fusarium coeruleum was the most dominant. These findings correspond with [54] who reported Fusarium coeruleum as the commonest Fusarium dry rot pathogen in potato. In addition, [55] reported higher cases of tuber rot due to Aspergillus niger attack in sweet potato tubers in Nigeria. However, [56] reported lower infection levels with Fusarium sambunicum isolated from stored seed potato. In addition, [57] reported a higher incidence of Rhizoctonia solani with up to 80% prevalence in Pakistan unlike 51.2% revealed in this study. Studies by [58] showed F. sambunicum as the most aggressive dry rot causing pathogen unlike Fusarium coeruleum reported this study. Fusarium dry rot was highly prevalent in farm saved tubers because the disease is seed borne and it's proliferated by poor post harvest handling practices. Dry rot causing pathogens including F. coeruleum, F. sambunicum and F .solani are all seed borne and infect healthy tubers through wounds at harvest [59]. A high infection level with black scurf was due to the pathogen being seed and soil borne. Highly viable seed and soil borne inocula of Rhizoctonia solani are carried in the soil or in infected tubers leading to spread of the disease [17,60,61]. Seed tubers infected with dry rot, black scurf and other fungal pathogens causes high storage losses through rotting and reduction in marketability of the ware potato due to formation of blemishes on skin of tubers [26,59,62].
Potato Virus S (PVS) was the most dominant virus infecting farm saved seed tubers. Both Potato Virus A (PVA) and Potato Virus M (PVM) tested negative. The proportion of samples infected with Potato Leaf Roll Virus (PLRV), Potato Virus X (PVX) and Potato Virus Y (PVY) were comparatively low. These results corresponds to [51] who reported high occurrence of Potato Virus S (80.1%) in Ethiopia and no positive samples for PVA were detected the study. However, [5] reported higher virus infection levels in East Africa. In addition, [63] working on screening of viruses for seed certification, reported higher occurrence of PVY and PVA unlike the results in this study. Higher occurrence of PVY was also reported in studies conducted in Tunisia [64]. Infection with viruses could be due to use of infected seed tubers from informal supply channels especially small tubers that have high viral loads [6,34]. Continuous use of the same seed potato without renewal leads to accumulation of seed borne pathogens including viruses [11]. Potato viruses are of phytosanitary importance in seed potato certification scheme and trade. Presence of viruses in seed tubers leads to production of smaller tubers and eventually yields loss [34,65]. Absence of PVA and PVM in farm saved seed tubers after detection using DAS-ELISA is not conclusive enough to ascertain that the samples were free from these two viruses. Studies have suggested use of molecular techniques such as Polymerase Chain Reaction (PCR) alongside DAS-ELISA so as to detect viruses even under low concentrations [66].

Conclusions and Recommendations
Most farmers rarely use certified seed and the informal seed system is the main channel for acquiring seed tubers. All famers use farm saved seed potato tubers and most store seed for two or three months in poor storage structures. Disease freeness is the main seed quality preference but farmers use tuber size in seed selection from the produce at harvest. Pests are a challenge in the field and also during storage leading to high losses. Farm saved seed potato is of poor health status with multiple infections with seed borne diseases. Farmers should be sensitized on use of recommended post-harvest handling practices to reduce seed losses. There should be increased supply of certified seed potato. Farmers should therefore be enlightened on the importance of use of certified seed potato for optimal potato production.