Open Access Peer-reviewed

Optimization of Culture Conditions Affecting Carboxy Methyl Cellulase Production by Aspergillus Species

K. V. Pavani1,, Gayathramma K.2, N.Sunil kumar1

1Department of Biotechnology, Gokaraju Rangaraju Institute of Engineering and Technology, Hyderabad, India

2Department of Biotechnology, Presidency College, Bangalore, India

World Journal of Agricultural Research. 2013, 1(4), 65-69. DOI: 10.12691/wjar-1-4-4
Published online: August 25, 2017

Abstract

The aim of this study was to determine the potential of new Aspergillus strain isolated from electroplating industry to produce Carboxymethyl Cellulase from agriculture waste. Agricultural wastes have great potential for the production of value added products with special reference to enzymes. Corn husk was used as substrate for Carboxymethyl Cellulase (CMCase) production by Aspergillus species through submerged fermentation. Optimization of parameters such as pH, temperature, substrate concentration was performed for the optimal production of CMCase. The fungal strain produced highest CMCase activity (3.3±0.01 IU/ml) at 5% (w/v) level of corn husk as substrate at 28°C and pH 8 over 72 hrs of incubation.

Keywords:

corn husk, Carboxymethyl Cellulase, agriculture waste, Aspergillus species
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