Open Access Peer-reviewed

Optimization of Culture Conditions Affecting Carboxy Methyl Cellulase Production by Aspergillus Species

K. V. Pavani1,, Gayathramma K.2, N.Sunil kumar1

1Department of Biotechnology, Gokaraju Rangaraju Institute of Engineering and Technology, Hyderabad, India

2Department of Biotechnology, Presidency College, Bangalore, India

World Journal of Agricultural Research. 2013, 1(4), 65-69. DOI: 10.12691/wjar-1-4-4
Published online: August 25, 2017


The aim of this study was to determine the potential of new Aspergillus strain isolated from electroplating industry to produce Carboxymethyl Cellulase from agriculture waste. Agricultural wastes have great potential for the production of value added products with special reference to enzymes. Corn husk was used as substrate for Carboxymethyl Cellulase (CMCase) production by Aspergillus species through submerged fermentation. Optimization of parameters such as pH, temperature, substrate concentration was performed for the optimal production of CMCase. The fungal strain produced highest CMCase activity (3.3±0.01 IU/ml) at 5% (w/v) level of corn husk as substrate at 28°C and pH 8 over 72 hrs of incubation.


corn husk, Carboxymethyl Cellulase, agriculture waste, Aspergillus species
[1]  Pandey, A., Soccol, C.R., Nigam, P., Soccol, V.T, “Biotechnological potential of agro- industrial residues: Sugarcane bagasse”, Biores Technol , 74 (1), 69-80, Aug, 2000.View Article
[2]  Ahuja, S.K., Ferreira, G.M., Moreira, A.R, Utilization of enzymes for environmental applications, Crit. Rev. Biotechnol, 24 (2-3),125-154, Apr,2004.View Article  PubMed
[3]  Kaur, J., Chadha, B.S., Kumar, B.A., Saini, H.S, “Purification and characterization of two endoglucanases from Melanocarpus sp. MTCC 3922”, Bioresour. Technol, Jan, 2007, 98 (1),74-81.View Article  PubMed
[4]  Bhat, M.K, “Cellulases and related enzymes in biotechnology”, Biotech. Adv, 18 (5), 355-383, Aug, 2000.View Article
[5]  Adsul,M.G., Bastawde, K.B., Varma, A.J., Gochale, D.V, “Strain improvement of Penicillium janthinellum M 1171 for increased cellulase production”, Bioresour. Technol , 98 (7),1467-1473, May,2007.View Article  PubMed
[6]  Harada, O., Lysenko, E.D., Edwards, N.M., Preston, K.R, “Effects of commercial hydrolytic enzyme additives on Japanese- style sponge and dough bread properties and processing characteristics”, Cereal Chem, 82 (3), 314-320, May/June, 2005.View Article
[7]  Dogaris, I., Vakontios, G., Kalogeries, E., Mamma, D., Kekos, D, “Induction of cellulases and hemicellulases from Neurospora crassa under solid- state cultivation for bioconversion of sorghum bagasse into ethanol”, Ind. Crops Product, 29 (2-3), 404-411, Mar, 2009.View Article
[8]  Acharya, P.B., Acharya, D.K., Modi, H.A, “Optimization for cellulase production by Aspergillus niger using sawdust as substrate”, Afr J Biotechnol, 7 (22), 4147 -4152, Nov, 2008.
[9]  Muhammad Irfan,Quratulain Syed,Muhammad Gulsher,Saijad Abbas, “Muhammad Nadeem,Shahjahan Baig. Pretreatment of corn cobs for the production of hydrolytic enzymes from Aspergillus niger-IR01”, IJAVMS, 4 (3), 81-87, Jul, 2010.
[10]  Muhammad Irfan, Saijad Abbas, Shahjahan Baig , Muhammad Gulshar,Muhammad Nadeem and Quratulain Syed, “Pretreatment: A Potential Technique to enhance the enzymatic hydrolysis”, World journal of Agricultural sciences, 6(4), 440- 445, Jul, 2010.
[11]  Sadia Aslam and Muhammad Asgher, “Partial purification and characterization of ligninolytic enzymes produced by Pleurotus ostreatusduring solid state fermentation”, African Journal of Biotechnology, 10 (77), 17875 - 17883, Dec,.2011.
[12]  Coral, G. B., Arikan, M. N., Unaldi and Guvenmez, H, “Some Properties of Crude Carboxymethyl Cellulase of Aspergillus Z10 Wild-Type Strain”, Turk. J. Biol, 26 (4), 209-213, Nov, 2002.
[13]  Pavani, K.V., Balakrishna, K.,Nagarjuna reddy Cheemarala, “Synthesis of zinc nanoparticles by Aspergillus species”, Int. j. of nanotechnology and applications, 51 (1),27-36,Jun,2011.
[14]  De-Moraes, L.M..P., Filho,. SA., and Ulhaa, C.J, “Purification and some properties of an alpha amylase and glucoamylase fusion protein from Saccharomyces cerevisiae”, World Journal of Microbiology and Biotechnology, World J. Microbiol. Biotechnol, 15 (5),561-564, Jul,1999.View Article
[15]  Peshin, A and Mathur J.M.S., “Purification and characterization of β-glucosidase from Aspergillus nigerstrain 322”, Lett. Appl. Microb, 28 (5), 401-404, May, 1999.View Article
[16]  Lowry, O.H., Rosebrough, N.J, Farr, A.L, Randall, R.J, Protein measurement with the Folin phenol reagent”, J. Biol. Chem. 193 (1) 265-275, Nov, 1951. PubMed
[17]  Ojumu, T.V., Solomon, B.O., Betiku, E., Layokun, S.K., Amigun, B, “Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101 fermented in sawdust, bagasse and corncob”, Afr. J. Biotechnol, 2 (6), 150-152, Jun, 2003.
[18]  Fadel, M, “Production physiology of cellulases and β-glucosidase enzymes of Aspergillus niger grown under solid state fermentation conditions”, Online Biol. Sci, 1 (5) 401-411, Jun, 2000.
[19]  Ghada A. Youssef, Physiological studies of cellulase complex enzymes of Aspergillus oryzae and characterization of carboxymethyl cellulase, African Journal of Microbiology Research, 5(11) 1311-1321, Jun, 2011.
[20]  Akiba S, Kimura Y, Yamamoto K, Kumaga PH ., “Purification and characterization of a protease- resistant cellulase from Aspergillus niger”, J.Fermen. Bioengin, 79 (2), 125-132, Jun, 1995.View Article
[21]  McCleary, B.V., Glennie- Holmes, M , “Enzymic quantification of (1-3) (1-4)-β-D-glucan in barley and malt”, J. Inst. Brew, 91 (3), 285-295, Jul, 1985.View Article
[22]  Ahmed, S., Aslam, N., Latif, Farooq., Rajoka, M. I., Jamil, A, “Molecular cloning ofcellulase genes from Trichoderma harzianum”, Frontiers in natural product chemistry, 1 (1), 73-75, Jan, 2005.View Article
[23]  Niranjane, A.P.,Madhou, P and Stevenson, T.W, The effect of carbohydrate carbon Sources on the production of cellulase by Phlebia gigantean. Enzyme and Microbial Technology Enzyme.Microbial.Technol. 40 (6), 1464-1468, May, 2007.