Open Access Peer-reviewed

Various Analytical Methodologies for Determination of Selective α1A Receptor Blocker Tamsulosin Hydrochloride and Its Combinations in Different Matrices

Alankar Shrivastava1,, Pratibha Aggrawal1

1Department of Pharmaceutical Analysis. Tifac-core Innovation square, B.R. Nahata College of Pharmacy, Mandsaur, India

World Journal of Analytical Chemistry. 2013, 1(3), 37-48. DOI: 10.12691/wjac-1-3-3
Published online: August 25, 2017


Tamsulosin is a more selective α1A subtype antagonist, which maintains the α-antagonist effect on the prostatic capsule and bladder neck but has less of an effect on the vascular system and blood pressure. It has a better side effect profile than earlier α-adrenergic-receptor antagonists, which were initially developed as antihypertensive agents. Tamsulosin hydrochloride is clinically important drug as far as benign prostatic hyperplasia is concerned. Thus in this review all of the analytical methods reported in the literature are summarized. Different spectrophotometric, chromatographic, electroanalytical and some other types of analytical methods were discussed here. Analytical methods were compared in terms of sensitivity, range, applications and economy. The presented review is helpful for the researchers involved in the development of new analytical methods or formulations of Tamsulosin hydrochloride.


tamsulosin hydrochoride, spectrophotometry, chromatography, analytical methods for tamsulosin hydrochloride, electroanalytical method for tamsulosin hydrochloride
[1]  Holtgrewe, H.L., “Current trends in management of men with lower urinary tract symptoms and benign prostatic hyperplasia” Urology 1998, 51(4A Suppl), 1-7.
[2]  Nagarathnam, D., Wetzel, J.M., Miao, S.W., et al. “Design and synthesis of novel alpha1a adrenoceptor-selective dihydropyridine antagonists for the treatment of benign prostatic hyperplasia” J Med Chem., 1998, 41, 5320-5333.View Article  PubMed
[3]  Shrivastava, A., Gupta, V.B., “Various treatment options for benign prostatic hyperplasia: A current update” J Midlife Health 2012, 3(1), 10-19. PubMed
[4]  Marberger, M, “Managing benign prostatic hyperplasia and prostate cancer – the challenges today” J Men's Health 2010, 7(2), 113-124.View Article
[5]  Ding, H., Du, W., Hou, Z.Z., Wang, H.Z., Wang, Z.P., “Silodosin is effective for treatment of LUTS in men with BPH: a systematic review” Asian J Andrology 2013, 15, 121-128.View Article  PubMed
[6]  Romics, I., “The role of alpha-adrenoreceptors in the treatment of urological diseases” Neurochemistry International 2007, 51, 328-331.View Article  PubMed
[7]  Fernando, M.A., Heaney, A.P. “α1-Adrenergic Receptor Antagonists: Novel Therapy for Pituitary Adenomas” Mol Endocrinology 2005, 19(12), 3085-3096.View Article  PubMed
[8]  Clifford, G.M., Farmer, R.D. “Medical therapy for benign prostatic hyperplasia: a review of the literature” Eur Urol 2000, 38, 2-19.View Article  PubMed
[9]  Harkaway, R.C., Issa, M.M. “Medical and minimally invasive therapies for the treatment of benign prostatic hyperplasia” Prostate Cancer and Prostatic Diseases 2006, 9, 204-214.View Article  PubMed
[10]  Acetti D, Brenna E, Fuganti C. A new enzymatic approach to (R)-Tamsulosin hydrochloride. Tetrahedron 2007, 18 (4), 488-492.View Article
[11]  Barbara, M. Synthesis of optically pure (r)-5-(2-aminopropyl)-2-methoxybenzenesulphonamide EP 1704140 A1 (text from WO2005063701A1)
[12]  Lowe, F.C. “Summary of Clinical Experiences With Tamsulosin for the Treatment of Benign Prostatic Hyperplasia” Reviews in Urology 2005, 7(Suppl 4), S13-S21. PubMed
[13]  Greco, K.A., McVary, K.T. “The role of combination medical therapy in benign prostatic hyperplasia” International J Impotence Research 2008, 20, S33-S43.View Article  PubMed
[14]  Analysis of drugs and poisons. Tamsulosin: alpha 1 adrenoreceptor blocker. Available online Assessed 21/03/13.
[15]  Shrivastava, A., Gupta, V.B. “A Review on Various Analytical Methods on Some Alpha Adrenergic Antagonists”. Current Pharmaceutical Analysis. 2011; 7: 27-41.View Article
[16]  European Pharmacopoeia. European Directorate for the Quality of Medicines. Strasbourg, 2007.
[17]  The Japanese Pharmacopoeia Sixteenth Edition. Available online
[18]  USP32–NF27. Pharmacopeial Forum: Volume No. 33(6) Page 1211.
[19]  British Pharmacopoeia. 2012. Available online
[20]  Van den Broeke, J., Langergraber, G., Weingartner, A. On-line and in-situ UV/vis spectroscopy for multi-parameter measurements: a brief review. Spectroscopy Europe 2006, 18(4), 15-18.
[21]  Naffrechoux, E., Fachinger, C., Suptil, J. Diode-array ultraviolet detector for continuous monitoring of water quality. Analytica Chimica Acta, 1992, 270, 187-193.View Article
[22]  Bosch Ojeda, C., Sanchez Rojas, F. Recent developments in derivative ultraviolet/visible absorption spectrophotometry. Analytica Chimica Acta 2004, 518, 1-24.View Article
[23]  Shrivastava, A., Gupta, V.B. “Stability-Indicating RP-HPLC Method for the Simultaneous Determination of Prazosin, Terazosin, and Doxazosin in Pharmaceutical Formulations” Sci Pharm. 2012, 80, 619-631.View Article  PubMed
[24]  Shrivastava, A. “Analytical methods for venlaflaxine hydrochloride and metabolites determinations in different matrices” Syst Rev Pharm 2012, 3, 42-50.View Article
[25]  Nanda, R.K., Gaikwad, J., Prakash, A. “Simultaneous Spectrophotometric Estimation of Tamsulosin in Pharmaceutical Dosage Form” Asian J. Research Chem. 2009, 2(1), 63-65.
[26]  Gadhve, N.A., Sawant, S.C., Ghante, M.R., Nikam, A.D. “Spectrophotometric estimation of tamsulosin hydrochloride in tablet dosage form” International J Pharmaceutical Research Development. 2011, 3(4), 87-92.
[27]  Bari, S.B., Bakshi, A.R., Jain, P.S., Surana, S. J. “Application of UV-Spectroscopy and First Order Derivative Method for Determination of Tamsulosin Hydrochloride in Bulk and Tablets” Pharm Anal Acta 2011, 2, 2.View Article
[28]  Shrivastava, A., Saxena, P., Gupta, V.B. “Spectrophotometric estimation of tamsulosin hydrochloride by acid-dye method” Pharm Methods 2011, 2, 53-60.View Article  PubMed
[29]  Chaudhari, B.G., Patel, N.U., Patel, D.B. “Spectrophotometric Method for Estimation of Tamsulosin Hydrochloride in Pharmaceutical Dosage Form Using Bromate-Bromide and Methyl Orange Reagent” International J Pharmaceutical Research Scholars. 2012, 1(3), 104-111.
[30]  Patel, D.B., Neelam. U. Patel, Chaudhari, B.G. “Validated spectrofluorimetric method for the determination of Tamsulosin Hydrochloride in tablet dosage form” Der Pharmacia Sinica, 2011, 2 (3): 172-175.
[31]  Raghubabu, K., Shanti swarup, L., Kalyanaramu, B., Rao, M.N., Ramdas, C. “Simple and inexpensive methods development for the estimation of Tamsulosin Hydrochloride as a single component from its solid dosage forms by visible spectrophotometry” International J Pharmacy Biological Sciences 2012, 2(1), 12-19.
[32]  Saradhi, S.V., Meherjaha, S.K., Jyothsna, N., Priyanka, A., Baby Sirisha, P., Ramakrishna, C., Sekaran B. Novel Spectroscopic Methods for the Ch.Determination of Tamsulosin in Bulk and Capsules. Journal of Pharmaceutical Science and Research. 2012, 4(11), 1958-1963.
[33]  Thimmaraju, M.K., Rao, V., Gurrala, S., Jayapal Reddy, G. “UV spectrophotometric method for simultaneous determination of Finasteride and Tamsulosin in combined dosage form” International J Pharmacy and Biological Sciences 2011, 1(3), 303-310.
[34]  Kategaonkar, A.H., Patel, D.M., Choudhari, V.P., Kuchekar, B.S., Nikalje, A.G. “Simultaneous determination of Finasteride and Tamsulosin in pharmaceutical preparations by ratio derivative spectroscopy” Journal of Pharmacy Research 2009, 2(6), 1065-1067.
[35]  Nanda, R.K., Gaikwad, J., Prakash, A. “Estimation of tamsulosin and tolterodine in its pharmaceutical dosage form by spectrophotometric method” International Journal PharmTech Research. 2009, 1(3), 420-423.
[36]  Choudhari, V.P., Gite, S.R., Raut, R.P., Hable, A.A., Parekar, S.R., Kuchekar, B.S. Spectrophotometric simultaneous determination of dutasteride and tamsulosin in combined tablet dosage form by first order derivative spectroscopy and area under curve (AUC) spectrophotometric methods and its application to uniformity of content in tablet and capsule. International Journal of Pharmaceutical Sciences Review Research. 2010, 2, 2, 63-67.
[37]  Nasare, M., Satish, J, Kumar, A.M., Akiful Haque, M., Prasad, V.V.L.N., Diwan, P.V. “UV Spectrophotometric Method for Simultaneous Determination of Tamsulosin and Finasteride in Combined Dosage Form” Am. J. PharmTech Res. 2012, 2(5), 781-788.
[38]  Nasare, M., Satish, J, Kumar, A.M., Akiful Haque, M., Prasad, V.V.L.N., Diwan, P.V. “Second derivative spectrophotometric method for simultaneous determination of tamsulosin and finasteride in pharmaceutical formulations” Asian J. Pharm. Ana. 2012, 2, 3, 73-76.
[39]  Shrivastava, A., Gupta, V.B. “Ultra violet spectrophotometric method: Not possible for the simultaneous estimation of alpha one adrenoreceptor blockers” Journal of Pharmaceutical Negative Results 2011, 2, 115-20.View Article
[40]  Lindholm, L. “Development and validation of HPLC method for analytical and preparative purposes” Volume 995 of Comprehensive summaries of Uppsala dissertations from the Faculty of Science and Technology. 2004, pp. 13, 14.
[41]  Shrivastava, A., Gupta, V.B. “HPLC: Isocratic or Gradient Elution and Assessment of linearity in Analytical Methods” J Adv Scient Res, 2012, 3(2), 12-20.
[42]  Srivastava, M.M. High Performance Thin Layer Chromatography (HPTLC). New York: Springer, 2011, Page 9.View Article
[43]  Attimarad, M., Mueen Ahmed, K.K., Aldhubaib, B.E., Harsha, S. “High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery” Pharm Methods 2011, 2, 71-5.View Article  PubMed
[44]  Fan, H.R., Gu, Y., Si, D.Y., Liu, C.X. “Determination of tamsulosin in dog plasma by a high sensitive liquid chromatography-tandem mass spectrometric method” Yao Xue Xue Bao. 2007, 42(8), 872-6. PubMed
[45]  Matsushima, H., Takanuki, K.I., Kamimura, H., Watanabe, T., Higuchi, S. “Highly sensitive method for the determination of tamsulosin hydrochloride in human plasma dialysate, plasma and urine by high-performance liquid chromatography-electrospray tandem mass spectrometry” J Chromatogr B Biomed Sci Appl 1997, 695(2), 317-27.View Article
[46]  Ding, L., Li, L., Tao, P., Yang, J., Zhang, Z. “Quantitation of tamsulosin in human plasma by liquid chromatography-electrospray ionization mass spectrometry” J Chromatogr B Analyt Technol Biomed Life Sci. 2002, 767(1), 75-81.View Article
[47]  Zhang, Z., Yang, G., Liang, G., Liu, H., Chen, Y “Chiral separation of tamsulosin isomers by HPLC using cellulose tris (3,5-dimethhylphenylcarbamate) as a chiral stationary phase” J Pharm Biomed Anal. 2004, 34(3), 689-93.View Article
[48]  Macek, J., Klíma, J., Ptácek, P. “Rapid determination of tamsulosin in human plasma by high-performance liquid chromatography using extraction with butyl acetate” J Chromatogr B Analyt Technol Biomed Life Sci. 2004, 809(2), 307-11. PubMed
[49]  Qi, M., Wang, P., Liu, L. “Determination of tamsulosin in dog plasma by liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry” J Chromatogr B Analyt Technol Biomed Life Sci. 2004, 805(1), 7-11.View Article  PubMed
[50]  Keski-Rahkonen, P., Pärssinen, O., Leppänen, E., Mauriala, T., Lehtonen, M., Auriola, S. “Determination of tamsulosin in human aqueous humor and serum by liquid chromatography-electrospray ionization tandem mass spectrometry” J Pharm Biomed Anal. 2007, 43(2), 606-12.View Article  PubMed
[51]  Nageswara Rao, R., Kumar Talluri, M.V., Narasa Raju, A., Shinde, D.D., Ramanjaneyulu, G.S. “Development of a validated RP-LC/ESI-MS-MS method for separation, identification and determination of related substances of tamsulosin in bulk drugs and formulations” J Pharm Biomed Anal. 2008, 46(1), 94-103.View Article  PubMed
[52]  Chandorkar, J.G., Kotwal, V.B., Dhande, N.S., Gurav, S.G., Pande, V.V., Yadav, P.V. “A Sensitive HPLC method for simultaneous estimation of tamsulosin hydrochloride and its impurity” Pakistan Journal of Pharmaceutical Science, 2008, 21(3), 307-310. PubMed
[53]  Choudhari, V.P., Nikalje, A.P.G. “Stability-Indicating HPTLC Method for the Determination of Tamsulosin in Pharmaceutical Dosage Forms” Chromatographia 2009, 69(11-12), 1463-1467.View Article
[54]  Basaveswara Rao, M.V., Reddy, B.C.K., Subba Rao, M., Sreedhar, B. “Development and validation of RP – HPLC method for the determination of Tamsulosin Hydrochloride” Int. J. Chem. Sci. 2008, 6(3), 1695-1701.
[55]  Nithiyananthan, T.S., Shankarananth, V., Rajasekhar, K.K., Ravikiran, P., Vikram Kumar, E., Jayanth kumar reddy G. “RP-HPLC method for the estimation of tamsulosin hydrochloride in bulk and tablet dosage form” Drug Invention Today 2009, 1(2),154-156.
[56]  Patel, D.B., Patel, N.J. “Validated stability indicating HPTLC method for the determination of Tamsulosin hydrochloride in pharmaceutical dosage forms” International J ChemTech Research 2010, 2(1), 646-652.
[57]  Thomas, S., Dhomane, J. “A Validated RP-HPLC Method for the Determination of impurities in Tamsulosin HCl” Int J Chem Res 2011, 2(4), 2933.
[58]  Aboul-Enein, H.Y., Hussein, R.F., Radwan, M.A., Yusuf, A., Al-Ahmadi, W., Al-Rawithi S. “Tamsulosin Dissolution from Pharmaceutical Dosage Forms Using an Automated HPLC System” J Liquid Chromatography & Related Technologies, 2003, 26:7, 1109-1116.View Article
[59]  Bari, S.B., Bakhshi, A.R., Jain, P.S., Surana, S.J. “Development and Validation of Stability-Indicating HPTLC Determination of Tamsulosin in Bulk and Pharmaceutical Dosage Form” Chromatography Research International. 2011.View Article
[60]  Thimmaraju, M.K., Rao, V., Hemanth, K., Siddartha, P. “RP HPLC Method for the determination of Tamsulosin in bulk and Pharmaceutical formulations” J Applied Pharmaceutical Science 2011, 1(8), 177-180.
[61]  Basniwal, P.K., Panda, S., Jain, S., Jain D. “Stability-indicating HPLC Assay Method and Degradation Profile of Tamsulosin” American-Eurasian J Scientific Research 2012, 7(5), 193-198.
[62]  Kantor-Boruta, M., Lisowska-Kuźmicz, M., Jończyk, A., Siedlecka, J., Ocios-Bębenek, A., Jarończyk, M., Mazurek, A.P., Ksycińska, H., Chilmonczyk, Z., Jarosz, M. “The new HPLC methodology for the chiral separation of tamsulosin enantiomers on amylase tris(3,5-dimethylphenylcarbamate) stationary phase” Talanta. 2012, 102, 75-78.View Article  PubMed
[63]  Choi, C.I., Lee, H.I., Bae, J.W., Lee, Y.J., Byeon, J.Y., Jang, C.G., Lee, S.Y. ‘Determination of tamsulosin in human plasma by liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study” J Chromatogr B Analyt Technol Biomed Life Sci. 2012, 909, 65-69.View Article  PubMed
[64]  Kumar, G.S., Kumar S.P. “Stability-Indicating RP-HPLC Method for Determination of Tamsulosin HCL in Pharmaceutical Dosage Form” J Basic Clinical Pharmacy 2012, 3(2), 255-260.
[65]  Basaveswara Rao, M.V., Reddy, B.C.K., Srinivas Rao, T., Jha A. “Developement and validation of dissolution test for tamsulosin hydrochloride pellets” Oriental J Chemistry 2008, 24(3), 1049-1052.
[66]  Kumari, R., Dash, P.P., Lal, V.K., Mishra, A., Murthy, P.N. “RP - HPLC method for the estimation of Tamsulosin Hydrochloride in Tablet Dosage Form” Indian J Pharm Sci. 2010, 72(6), 785-7.View Article  PubMed
[67]  Ksycińska, H., Rudzki P.J. “LC-MS Determination of Tamsulosin in Human Plasma” Available online
[68]  Ksycińska, H., Rudzki, P.J., Sarosiek, A. “Validated LC-MS method for determination of tamsulosin in human plasma and its application to pharmacokinetic study” Acta Pol Pharm. 2006, 63(5), 417-9. PubMed
[69]  Siva Rama Krishna, G.V., Janardhan, M., Rasool, S. “Development and validation of stability-indicating RP-HPLC method for estimation of tamsulosin HCl pellets” International J Pharmaceutical Invention 2012, 2(7), 51-60.
[70]  Jain, P.S., Chaudhari, A.J., Bari, P.R., Surana, S.J. “Validated stability-indicating RP-HPLC method for tamsulosin hydrochloride in pharmaceutical dosage form according to ICH guidelines: Application to stability studies” Der Pharmacia Lettre, 2012, 4 (6), 1760-1767.
[71]  Ramakrishna, N.V.S., Vishwottam, N., Manoj, S., Koteshwara, M., Wishu, S., Varma, D.P. “Rapid, simple and highly sensitive LC-ESI-MS/MS method for the quantification of tamsulosin in human plasma” Biomed Chromatography 2005, 19(10), 709-719.View Article  PubMed
[72]  Study on the application of HPLC in pharmaceutical quality control of tamsulosin hydrochloride, yankening tablets and yujin yinxie tablets. Available online:
[73]  Yan-hong, W, Zhong-zhou, C., Yuan LI Hua, G. HPLC determination of content and related substances of tamsulosin hydrochloride and its sustained release capsules. Chinese J Pharmaceutical Analysis. Available online
[74]  Chambers, E.E., Diehl, D.M. A Highly Sensitive Method for the Analysis of Tamsulosin (Flomax) in Human Plasma. Chromatography online. Available online
[75]  Meiling, Qi., Wang, P., Cong R. “Determination of the Enantiomers of Tamsulosin Hydrochloride and its Synthetic Intermediates by Chiral Liquid Chromatography” Chromatographia 2004, 59(3-4), 251-254.
[76]  Srinivasu, M.K., Rao, B.M., Vittal T.V.S.K., Rajendra Kumar P., Chandrashekhar K.B. “A validated chiral LC methods for the enantioselective analysis of Tamsulosin Hydrochloride and its eneatiomers (S)-5-[2-[[2-(O-Ethoxyphenoxy)ethyl]amino]propyl]-2-methoxybenzenesulphonamide, monohydrochloride on amylase based stationary phase” Indian Drugs 2006, 42(2), 230-237.
[77]  Rao B.M., Srinivasu M.K., Sridhar G., Reddy B.S.S., Vittal T.V.S.K., Kumar R.P., “Chandrashekhar K.B. HPLC studies on the stress degradation behavior of tamsulosin hydrochloride and development of validated specific stability indication method” Indian Drugs 2005, 43(1), 39-43.
[78]  Agarwal, S., Gowda, K.V., Sarkar, A.K., Ghosh, D., Bhaumik, U., Chattaraj, T.K., T.K, Pal. Simultaneous Determination of Tamsulosin and Dutasteride in Human Plasma by LC–MS–MS. Chromatographia 2008, 67, 893-903.View Article
[79]  Patel, D.B., Patel, N.J. Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms. Acta Pharm. 2010, 60, 197-205.View Article  PubMed
[80]  Thimmaraju, M.K., Rao, V., Gurrala S. “RP HPLC method for the determination of finasteride and tamsulosin in bulk and pharmaceutical formulations” Der Pharmacia Lettre, 2011, 3(5), 79-86.
[81]  Bari, S.B., Jain, P.S., Bakshi A.R., Surana, S.J. “HPTLC Method Validation for simultaneous determination of Tamsulosin Hydrochloride and Finasteride in Bulk and Pharmaceutical Dosage Form” J Anal Bioanal Techniques 2011, 2, 2.View Article
[82]  Sindhura, M., Raghavi, K., Prashanthi, R., Nalluri, B.N. “Simultaneous Estimation of Finasteride and Tamsulosin Hydrochloride in Combined Dosage Forms by RP-HPLC-PDA Method” J Applied Pharmaceutical Science 2012, 2(6), 203-209.
[83]  Shrivastava, A., Gupta, V.B. “Validated HPLC and HPTLC Methods for Simultaneous Determination of Some α1-Adrenoreceptor Blockers” Lat. Am. J. Pharm. 2012, 31 (2), 279-86.
[84]  Gauri Shankar, D., Durvasa Rao, B., Sai Kishore, V., Murthy, T.E.G.K. “Simultaneous determination of tamsulosin hydrochloride and finateride in formulations by reverse phase-HPLC” Asian J Chem., 2007, 19(2), 1375-1378.
[85]  Jain, P.S., Bakhshi, A.R., Bari, S.B., Surana, S.J., Chaudhari, A.J. “RP-HPLC assay method for simultaneous estimation of tamsulosin hydrochloride and finasteride in pharmaceutical dosage form” Analytical chemistry: An Indian Journal 2012, 11(5).
[86]  Shrivastava, A., Sharma, J., Soni, V. “Various electroanalytical methods for the determination of uranium in different matrices” Bulletin of Faculty of Pharmacy, Cairo University, 2012.
[87]  Ozkan, S.A., Uslu, B., Aboul-Enein, H.Y. “Voltammetric investigation of Tamsulosin” Talanta 2003, 61(2), 147-56.View Article
[88]  Maier, V., Horáková, J., Petr, J., Tesarová, E., Coufal, P., Sevcík, J. “Chiral separation of tamsulosin by capillary electrophoresis” J Pharm Biomed Anal. 2005, 39(3-4), 691-6.View Article  PubMed
[89]  Kavalírová, A., Pospísilová, M., Karlícek, R. “Enantiomeric purity determination of tamsulosin by capillary electrophoresis using cyclodextrins and a polyacrylamide-coated capillary” Farmaco 2005, 60(10), 834-9.View Article  PubMed
[90]  Zhang, Y.P., Zhang, Y.J., Gong, W.J., Wang, S.M., Xue, H.Y., Lee, K.P. “Design of Experiments for Capillary Electrophoretic Enantioresolution of Tamsulosin using Sulfated-β-Cyclodextrin as Chiral Selector” J Liquid Chromatography & Related Technologies, 2007, 30(2), 215-234.View Article
[91]  Abdel-Moety, M.M., Hassan N.Y.M., Abdel-Aleem, Abdel-Aziz A., Abdel-Hamid, S.G. “Determination of Alfuzosin hydrochloride and Tamsulosin hydrochloride in pure state and pharmaceutical preparations by conductimetric methods” J Chemical and Pharmaceutical Research, 2012, 4(7), 3740-3748.
[92]  Petr, J., Maier, V., Horáková, J., Sevcík, J. “Simultaneous contactless conductivity detection and UV detection for the study of separation of tamsulosin enantiomers in discontinuous electrolyte systems by CE” Electrophoresis. 2006, 23, 4735-45.View Article  PubMed
[93]  Lonappan, L., Issac, S., Joseph, R., Thomas, D., Girish Kumar, K. Electrochemical studies of tamsulosin hydrochloride using multiwalled carbon nanotube-modified glassy carbon sensor. Micro & Nano Letters, 2011, 6(10), 867-870.View Article
[94]  Mierzejewska, D., Marciniak-Darmochwał, K., Kostyra, H., Rudnicka, B. “Application of the conductometric method for differentiation of proteins and peptides” Pol. J. Food Nutr. Sci. 2007, 57(1), 77-82.
[95]  Khopkar, S.M. Basic Concepts of Analytical Chemistry. Page 431.
[96]  Dupont, P.F. Introduction to common laboratory assay and technology. In ed: Mary Lee. Basic Skills in Interpreting Laboratory Data. pp 24.
[97]  Wang, J. Analytical Electrochemistry. Third edition. 2006. John Wiley and Sons. pp 165.View Article
[98]  Whatley H. Basic Principles and Modes of Capillary Electrophoresis. Edited by: Petersen JR and Mohammad AA. In Clinical and Forensic Applications of Capillary Electrophoresis. Humana Press Inc., Totowa, NJ. pp 21. PubMed
[99]  Glynn, J.R., Belongia, B.M., Arnold, R.G., Ogden, K.L., Baygents, J.C. “Capillary Electrophoresis Measurements of Electrophoretic Mobility for Colloidal Particles of Biological Interest” Appl Environ Microbiol. 1998 July; 64(7): 2572-2577. PubMed
[100]  Taguchi, K., Schäfers, R.F., Michel, M.C. “Radioreceptor assay analysis of tamsulosin and terazosin pharmacokinetics” Br J Clin Pharmacol 1998; 45: 49-55.View Article  PubMed
[101]  Maier, N.M., Franco, P., Lindner, W. “Separation of enantiomers: Needs, challenges, perspectives” J Chromatogr A 2001, 906, 3-33.View Article
[102]  Shrivastava, A., Gupta, V.B. “Methods for the determination of limit of detection and limit of quantitation of the analytical methods” Chron Young Sci 2011, 2, 21-5.View Article