Figure 3. Plant mixture decreased HNKC apoptosis following exposure to apoptotic UVB dose. HNKC were first pre-treated during 3h with MIX before irradiation with UVB (60 or 100mJ cm-2). HNKC were analysed 24h later for the percentage of pro-apoptotic (Annexine+, IP-), apoptotic (Annexine+, IP+), and necrotic (Annexine-, IP+). In contrast to UVB60, higher dose (UVB100) increased the percentage of apoptotic cells and cell death. Pretreatment with MIX significantly reversed cell apoptosis/necrosis at lower extract dilutions (3, 10 and 6µg/ml of AA, PI and PR respectively). Data show mean±SD from 3 distinct HNKC preparations


Sublethal UVB Induces DNA Lesions and Pro-Apoptotic Gene Transcription in Human Keratinocytes: Attenuation by a Mixture of Plant Extracts

Rachid Ennamany, Nadine Leconte, Jacques Lecrlerc, Aliza Jabès, Hamid-Reza Rezvani, Jérôme Rambert, Khaled Ezzedine, M. Djavad Mossalayi

World Journal of Preventive Medicine. 2013, 1(2), 4-10 doi:10.12691/jpm-1-2-1