Acute Metabolic and Enjoyment Responses of Three Walking Protocols

Encouraging physical activity is a key component of public health. The purpose of this study was to test the hypothesis that interval walking would produce higher 2 ) and similar enjoyment responses during and following exercise compared to continuous walking. Ten healthy adults (4 women, 6 men; mean age = 24 ± 5 years) completed the following 3 walking bouts in counterbalanced order and equated for total volume (90 MET·min): 1) 30 min of low-moderate continuous walking (3 METs; ~ 4.8 km/h), 2) 24 min and 24 s of interval walking (IW1) with cycles of 30 s:60 s of high-moderate (5 METs; ~ 6.4 km/h):low-moderate intensities, and 3) 26 min and 20 s of interval walking (IW2) with cycles of 30 s:120 s of high-moderate:low-moderate intensities. Accumulated O 2 uptake during exercise was higher during IW2 (28,232 ± 2,782 mL) compared to IW1 (26,561 ± 2,685 mL; p = 0.03) and continuous walking (24,500 ± 2,427 mL; p = 0.001), and higher during IW1 than during continuous walking (p = 0.001). EPOC over 20 min was higher after IW1 (1,268 ± 117 mL O 2 ) compared to continuous walking (892 ± 73 mL; p = 0.04); the 2 interval walking protocols were not different (IW2: 1, 2 and EPOC in shorter total durations of exercise compared to continuous walking of a similar enjoyment and volume.


Introduction
Physical inactivity in adults today is a tremendous public health challenge that contributes to excess fat accumulation and an increase in the prevalence and incidence of chronic disease, e.g., type II diabetes, cardiovascular disease, hyperglycemia, and obesity [22]. In 2011, only 52% of U.S. adults met minimum physical activity guidelines [12]. Americans who do not meet the physical activity recommendations often cite lack of time as a major barrier [5]. Interval training, however, has been shown to provide greater or similar benefits to traditional continuous training in a shorter time [5,9,18] and may represent a time-efficient strategy to increase physical activity [16]. 2max ) and increased muscle glycogen and maximal activity of citrate synthase [8,9] associated with interval training compared to traditional continuous training, regardless of age, sex, or health status [8,9,18,24,31]. In addition, Selfridge [31] noted high intensity interval training to be more effective for increasing maximum exercise capacity, improving blood pressure, reducing body fat, and raising HDL-C than continuous moderate exercise.
--2max ) it places great strain on the cardiovascular system and may be contraindicated for some adults [10]. Per Stanley and Cumming [34], the structure of an exercise bout can influence the type and duration of elicited exercise related affect responses. Moreover, negative post-exercise affect responses reduces exercise adherence and have been linked to high intensity interval training [27,37,38]. Accordingly, lower intensity interval training protocols have been studied and investigators have shown that they can effectively improve affect [36].
One such form of lower intensity exercise that can be performed using intervals is walking. Walking is a common and practical form of weight-bearing exercise that reduces life-style related risks of chronic diseases and increases positive-activated affect which is linked to increased exercise adherence [15,34]. Traditional interval walking training is a type of interval training that consists of 3-≥70% peak oxygen uptake, O 2peak ) and l ≤ 2peak ) walking intensities [21,23]. Because interval walking training intensities are lower than most traditional interval training protocols, it may reduce injury risk and improve exercise adherence. To date, interval walking training has been shown to improve body mass index, 2peak , knee extension and flexion strength, and blood pressure when compared to continuous walking [19,21,23,29]. Although intermittent, a modified interval -2peak -2peak ) exercise was utilized by Campbell et al. [10], who found a reduction in low density lipoprotein.
Some health effects associated with interval walking may be attributed to excess post-exercise oxygen consumption (EPOC), defined as the elevated rate of oxygen uptake above the resting value after exercise [30]. In a study conducted by Chan and Burns [13], EPOC values were found to be 43% higher than resting values over a 2-hour period following sprint-interval exercises. According to Børsheim and Bahr [7] ' -2 during exercise and higher EPOC after exercise may contribute to an increased caloric deficit and positive affect in adults. -2 during exercise and EPOC values following exercise, but the ideal exercise and activ 2 during exercise, EPOC, and exercise enjoyment remains unclear. Therefore, the purpose of this study was 2 and similar enjoyment responses during and following exercise compared to continuous walking.

Study Participants
Ten adults (4 women and 6 men) aged 19-36 volunteered to participate. Each participant completed a medical history and Physical Activity Readiness Questionnaire to screen for health risks that would preclude participation in moderate-intensity exercise. Any person classified as high risk-using American College of Sports Medicine [3] criteria-was excluded from the study. All procedures were approved by the local Institutional Review Board and subjects provided written informed consent prior to participation.

Experimental Design
Participants reported to the laboratory on 4 separate occasions; all participants were instructed to refrain from caffeine, alcohol, and food consumption for at least 2 h prior to each visit and avoid moderate to vigorous exercise for 24 h prior to each visit. A 24-h history form was used to verify adherence to these guidelines.
The modified Balke-Ware Treadmill Protocol [4] and the International Physical Activity Questionnaire (short form) [14] were administered during the initial visit to ' tivity levels, respectively. Because this type of training would be most appropriate for less-fit individuals, participants with ≥ th percentile via the Balke-Ware Treadmill Protocol were excluded from the study. Height (Seca, Gayamills, WI), weight (Tanita BWB-800, Japan), waist and hip circumference (Gulick II Measuring Tape, Gayamills, WI), and percent body fat was estimated via 3-site skinfolds [17] were measured for each participant.
After the initial session, participants returned 2 days later to complete the first of 3 experimental trials. Each q ≥ The experimental trials consisted of 3 treatments: 1 continuous walking and 2 interval walking protocols performed on a treadmill (Quinton, Seattle, WA) in a temperate environment (~22-23°C). Each visit occurred at the same time of day (± 1 h), and the order in which the treatments were performed were counterbalanced with the counterbalanced treatment orders randomly assigned.
MET· mins were used to equate total estimated energy expenditure during the treadmill protocols within participants. Additionally, moderate exercise intensities (3-5 METs) [1,2] were chosen to maximize external and ecological validity. The continuous walking protocol consisted of participants walking continuously for 30 min at a low-moderate workload of 3 METs (~ 4.8 km/h) [1,2]. Interval walking protocols consisted of cycled bouts of high-moderate and low-moderate intensities. Each interval walking work bout was set at a high-moderate intensity of 5 METs (6.4 km/h) [1,2] ‗ ' -moderate 3 M / ‗ ' first interval walking protocol (IW1) consisted of a 24 min and 24s bout of walking (19% shorter duration than continuous walking) and work:active recovery bouts of 30 s:60s. The second interval walking protocol (IW2) consisted of work:active recovery bouts of 30s: 120s totaling 26 min and 20 s (11% shorter duration than continuous walking). Each protocol consisted of the same volume of 90 MET· min. Figure 1 summarizes the exercise protocols.

Calculations
2 ) and respiratory quotient (RQ) were measured using a metabolic cart (TrueOne 2400, Parvo Medics, Sandy, UT) -2 and RQ were recorded continuously, averaged every 30 s, and calculated as the average of the final 5 min [35]. In addition, pre-exercise enjoyment [7-point exercise enjoyment scale ranging from 1 (not at all) to 7 (extremely)] [33] -2 , RQ, and heart rate (HR; Polar, Lake Success, NY) were averaged. These measurements, rating of perceived exertion (RPE; using the 6-20 Borg scale) [6], and enjoyment during exercise were measured at 6 time points, evenly distributed amongst each protocol (Continuous walking: 5 min, 10 2 , RQ, and HR were measured and averaged every 5 min and enjoyment and overall RPE (How hard did you work during the previous exercise session?) were measured during the initial 5 min of a 20-min supine rest period [26] 2 2 curve via the trapezium rule by adding the areas under the graph between each pair of consecutive observations [20]. Utilizing RQ, total-, fat-and carbohydrate-accumulated caloric expenditure, and relative substrate contributions were calculated [11].

Statistical Analyses
2 -exercise; caloric expenditure; and HR, RPE, and enjoyment during and post-exercise. Five-min cumulative EPOC values were analyzed between treatments using a two-way repeated measures ANOVA (treatment × time). Bonferroni post-hoc comparisons were used, when applicable, to determine individual differences between treatments. All data were analyzed using SPSS v. 22 (Statistical Package Y α = used for all hypothesis tests.

Journal of Physical Activity Research
IW1 resulted in higher accumulated 20-min EPOC than continuous walking (1,268 ± 117 mL vs. 892 ± 73 mL; p < 0.05), but EPOC after IW2 (1,174 ± 178 mL) was not different than the other protocols. Figure 4 shows the EPOC following each exercise bout. IW1 elicited a higher EP = 2 at 10 min post exercise.

Discussion
This is the first study investigating both metabolic and enjoyment responses of continuous and interval walking of similar volume before, during, and following exercise. In support of our hypothesis, the primary findings were that the interval walking protocols-despite being comprised of shorter durations than continuous walking (IW1: 19% shorter duration; IW2: 11% shorter duration)-resulted in greater accumulated O 2 uptake during exercise (IW1: 8%; IW2: 13%) and greater EPOC (IW1: 30%; IW2: 24%). It should be noted that though the differences among walking protocols were statistically significant, they were small. However, it is also important to note that small differences, when repeated often, can accumulate into substantial total differences over time.
Interestingly, the more intense, moderate intensity interval walking protocols produced similar exercise enjoyment and RPE as continuous walking. These findings are similar to a previous field experiment conducted by our laboratory (unpublished observations) that investigated the self-reported enjoyment responses of 3 self-selected intensity walking protocols [1 continuous walking protocol at a low-moderate intensity (RPE-12) and 2 interval walking protocols with work bouts set at 30s high-moderate (RPE-13): 60 s low-moderate and 30s high-moderate: 120s lowmoderate intensities] of constant duration (20 min) in adult females. As with the current findings, we found no enjoyment or RPE differences between the continuous and interval walking protocols.
Previous affect research has found high intensity interval training to produce negative feeling responses in comparison to continuous training during and after exercise [28]. Conversely, greater positive affect is evident after acute bouts of moderate intensity exercise [33,36]. Since the highest intensity used during the intervals in the present study was only moderate, it may explain why the interval protocols resulted in similar enjoyment ratings as the continuous exercise that was performed at a lower intensity. In addition, similar enjoyment among treatments could be attributed to the fact that participants were healthy and of similar physical fitness levels. This notion could explain why even though exercise intensity was moderate, perceived exertion was equivalent to what is ≤ [3]. * Similar to previous high intensity interval training exercise ratios (30 s) [16,38], our interval walking protocols e 2 [22] 3 -2 during the exercise portion of the interval walking protocols. It was unexpected that lowering the work-to-rest ratio from 1:2 (IW1: 30 s/60 s) to 1:4 (IW2: 30 s/120 s) would elicit significantly greater energy expenditure, where the duration (and number) of 5-MET exercise bouts was 35% lower than IW1 of the same work volume. Since exercise HR was similar between 2 . EPOC is comprised of rapid and slow components. These results support the notion that exercise intensity may be of more importance than duration in terms of influencing EPOC [7]. It may be that higher EPOC following interval walking was because of greater disturbance to homeostasis; thus, during the rapid component of EPOC, greater restoration of both phosphocreatine and oxygen stores in the muscle was necessary. Similarly, elevated levels of epinephrine or norepinephrine following interval walking may account for the greater EPOC. In contrast to the fast component -2 to near baseline following the initial 5 min post-exercise period.
The interval protocols elicited 9% (IW1) and 13% (IW2) greater cumulative total-accumulated caloric expenditures than continuous walking. Additionally, per minute, total-accumulated caloric expenditure was greater during the interval walking protocols than continuous walking and may be more effective in expending calories when time available for physical activity is limited. Moreover, cumulative fat-accumulated caloric expenditure during IW2 was similar to continuous walking and 20% greater than IW1. This finding is comparable to those who have noted greater fat oxidation following interval exercises of high intensities (Chan and Burns 2013). Although IW2 was of greater duration than IW1, it was less intense and may be a more suitable form of interval walking for fat oxidation in sedentary adults.
Walking is the most commonly preferred form of physical activity of adults that meet the physical activity guidelines [32]. The moderate intensity interval walking protocols resulted in similar enjoyment and RPE as the longer duration continuous walking, so it is 2 and EPOC associated with interval walking in the present study provide rationale for prescribing its use as a suitable substitute for conventional continuous walking.

Conclusion
In summary, findings from this study demonstrate that interval walking of various moderate intensities significantly 2 during and after exercise compared to continuous walking of the same work volume. The ideal dose of exercise and active recovery for lower intensity interval walking has not been clearly established. Therefore, further research should determine if moderate-intensity interval walking of various work-to-rest ratios have similar effects in sedentary and/or obese adults who cannot attain high levels of exercise intensity using traditional interval training in both laboratory as well as in field settings. Practitioners may find the results especially relevant for clinical populations for whom walking exercise is prescribed and who desire increased caloric expenditure despite a small time committable to exercise.