Pesticidal Activity of Phytolacca Dodecandra Extracts against Sitophilus ...

Michael Qwarse, Matobola J. Mihale, Leonia Henry, Joseph Sempombe, Veronica Mugoyela, Fortunatus Sung'hwa

Journal of Food Security

Pesticidal Activity of Phytolacca Dodecandra Extracts against Sitophilus zeamais (Motschulsky) (Curculionidae) and Tribolium castaneum (Tenebrionidae) Storage Pests in Maize

Michael Qwarse1, Matobola J. Mihale1,, Leonia Henry2, Joseph Sempombe3, Veronica Mugoyela3, Fortunatus Sung'hwa4

1Department of Physical Sciences, Open University of Tanzania (OUT), Dar es Salaam, Tanzania

2Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology, Dar es Salaam, Tanzania

3Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences (MUHAS), Dar es Salaam, Tanzania

4Department of Chemistry, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania

Abstract

This study assessed the pesticidal activities of Phytolacca dodecandra extracts in controlling Sitophilus zeamais and Tribolium castaneum pests in stored maize grains. The study was conducted at ambient conditions (25–30°C and 65–70% relative humidity) and designed in a completely randomised design with 8 treatments in triplicate. Contact toxicity, percent mortality, feeding deterrence and grains weight loss were assessed using developed procedures for 21 days and first filial generation progeny was assessed for 42 days of post-pest introduction. Both ethanolic root and leaf extracts of P. dodecandra demonstrated significant bioactivities against both S. zeamais and T. castaneum, with the leaf extract being more active than the root extracts. P. dodecandra leaf extracts killed 98% of S. zeamais and 99% of T. castaneum at concentration of 150 mg/mL on day 3. Similarly, P. dodecandra leaf extracts reduced grain damage to 0% and had moderate repellence of 57% and 66% to S. zeamais and T. castaneum, respectively. Hence, after biosafety studies this plant-based products can be included in the Integrated Pest Management strategies and can be recommended as an alternative remedy in treating maize storage pests in subsistence agriculture. Further isolation and structure elucidation of bioactive ingredients in the plant species are recommended and are underway.

Cite this article:

  • Michael Qwarse, Matobola J. Mihale, Leonia Henry, Joseph Sempombe, Veronica Mugoyela, Fortunatus Sung'hwa. Pesticidal Activity of Phytolacca Dodecandra Extracts against Sitophilus zeamais (Motschulsky) (Curculionidae) and Tribolium castaneum (Tenebrionidae) Storage Pests in Maize. Journal of Food Security. Vol. 4, No. 6, 2016, pp 147-155. http://pubs.sciepub.com/jfs/4/6/4
  • Qwarse, Michael, et al. "Pesticidal Activity of Phytolacca Dodecandra Extracts against Sitophilus zeamais (Motschulsky) (Curculionidae) and Tribolium castaneum (Tenebrionidae) Storage Pests in Maize." Journal of Food Security 4.6 (2016): 147-155.
  • Qwarse, M. , Mihale, M. J. , Henry, L. , Sempombe, J. , Mugoyela, V. , & Sung'hwa, F. (2016). Pesticidal Activity of Phytolacca Dodecandra Extracts against Sitophilus zeamais (Motschulsky) (Curculionidae) and Tribolium castaneum (Tenebrionidae) Storage Pests in Maize. Journal of Food Security, 4(6), 147-155.
  • Qwarse, Michael, Matobola J. Mihale, Leonia Henry, Joseph Sempombe, Veronica Mugoyela, and Fortunatus Sung'hwa. "Pesticidal Activity of Phytolacca Dodecandra Extracts against Sitophilus zeamais (Motschulsky) (Curculionidae) and Tribolium castaneum (Tenebrionidae) Storage Pests in Maize." Journal of Food Security 4, no. 6 (2016): 147-155.

Import into BibTeX Import into EndNote Import into RefMan Import into RefWorks

At a glance: Figures

1. Introduction

The agricultural sector in developing countries is heavily facing post-harvest crop losses in quality and quantity caused by storage pests [1]. This contributes to the problem of food shortages and poverty. Storage insects cause qualitative and quantitative pre- and post–harvest losses varying in magnitude from 10% to 100% in tropical countries [2, 3]. These losses are mainly caused by Sitophilus spp. Tribollium castaneum, Rhyzopertha dominica, Callosobruchus chinensis and Sitotroga cerealella in cereals, as well as Acanthoscelides spp and Callosobruchus spp in legumes [4, 5]. More damage in cereal crops is also caused by tenebrionid beetles and Prostephanus truncatus in stored cereal crops and cassava [6]. Currently synthentic pesticides are used for controlling storage pests. However, high costs, low biodegradibility, multi resistance to pests and disease causing microorganisms as well as their unavailability in rural areas make the use of synthetic pesticides uneconomical and incompatible in subsistence agriculture. As a result, plants and fungi have been used in pest control [2, 7].

Tanzania alone is endowed with a great abundance of flora diversity which is estimated to constitute about 10,000 vascular plant species, of which 25 % are indigenous and among them, about 1,200 species have been reported to occur exclusively in Tanzania [8, 9]. Northern part of Tanzania, Mbulu in particular, is well known to have abundance of the natural herbs of which their biological potential is not yet explored [10, 11]. A number of botanical plants in the region are known of their insecticidal properties by subsistence farmers, but there is no much scientific knowledge on their bioactivity and toxicity values. Lack of technical resources and assistance to the majority of subsistence farmers undermine their efforts in diseases and pests management at family level. Consequently, most of bioactive plants used traditionally have not been tested scientifically for their efficacy and toxicity in both human and animals. These cause the loss of crops quality and animal products which may affect the food security and livehood of many poor communities [2, 7].

Phytolacca dodecandra (L’ Herit) (synonyms: P. abyssinica Hoffin, Pircunia abyssinica Moq.) is a perennial herb of the Phytolaccaceae family, which is a climber plant growing rapidly with hanging branches. It has average height of 2 to 3 meters, although it can reach a height of up to 10 meters. The leaves of P. dodecandra are pinnate, opposite appearing whorled, ovate with 10-15 cm long, 4-12 cm broad with margins entire and undulating. Flowers of the plant are inflorescences, stand auxiliary, in racemes, rapidly symmetric and hypogynic. The sepals are rounded and fruit are reddish. P. dodecandra is distributed in East, West, Central and Southern Africa and some parts of South America and Asia [12, 13, 14].

The P. dodecandra is traditionally used in Ethiopia to control fresh water snails (as molluscicides) and to treat schistosomiasis. The species contains 25% of saponins in berries in which active molluscicide lemmatoxin was isolated [15]. The plant species has been used by subsistence farmers around Lake Victoria [2, 7, 14] to treat crops in the field and storage. Moreover, the plant powders have been used to produce laundry detergents and to control vectors such as mosquito larvae, housefly, and parasites such as mice [2, 16].

The maize weevil, Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae), is one of the most destructive pest which affect stored cereal crops in tropical areas [17], [18]. Weevil adults attack the grains then larvae cryptically feed and develop within grains [19]. Infestation by this weevil begins in the field [20], but most damage occurs during storage. Damaged grains caused by this weevil appear to be reduced in nutritional value, germination, weight, and commercial value [21]. The maize weevil can be conventionally controlled by residual pesticides and fumigation [19]. There has been decision to ban dangerous fumigants like Methyl bromide in developed countries since 2005 and in developing countries the plan was extended to 2015. These provide a wider room for natural pesticides investigation [22, 23].

The rust red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) is a cosmopolitan insect that infest on dried material of animal and plant origin, especially cereal grains and oil seeds. They can only eat damaged or milled grain and are often introduced to a warehouse or production facility through infested flour deliveries [17]. In particular, T. castaneum attack stored products, especially food grains and other food products including flour, grinded cereals, nuts and beans [24]. The red flour beetle originated from Indo-Australia. The adult can live up to three years causing enormous grain damage and loss of stored products.

The purpose of this study was to evaluate the biopesticidal activities (contact toxicity, feeding deterrence and repellent effects) of P. dodecandra extracts, commonly used traditionally by agro-pastoralists in Mbulu district for pest management. The study intended to scientifically assess the pesticidal efficacy of crude leaf and root extracts of P. dodecandra against S. zeamais (Motsch.) and T. castaneum storage pests. Hence, the study will form a baseline for further research on the isolation and chemical characterization of the active ingredients against storage pests in the plant species. This will lead to have effective and affordable pesticides which are environmentally safe for use by farmers in subsistence agriculture.

2. Materials and Methods

2.1. Study Area

The study was carried out in Mbulu district, south western part of Tanzania (Figure 1). The area of this district is approximately 7,695 square km (including Lake Eyasi) of which the dry land is approximately 6,700 square km. The altitude of the district ranges from 1,110 m to 2,250 m. This difference in altitude contributes to the wide range of climatic conditions with mean annual temperature and rainfall ranging between 17.3°C and 23.4°C and from 400 mm to 1,100 mm, respectively.

Figure 1. Map of Tanzania showing Mbulu District (Source: Modified from Google Earth)

Mbulu district is mainly dominated by hunters and agro-pastoral communities. There are some other socio-economic activities practiced such as afforestation, extensive grazing and smallholder rainfed cultivation as well as mechanized rainfed cultivation with medium to high inputs. The main crops grown in the study area include maize, beans, pigeon pea, sorghum, wheat, vegetable, fruits and coffee [25]. It was reported that, P. dodecandra leaves are used by subsistence farmers in Mbulu district to control grain pests in stored grains with minimal loss of quantity and quality [14].

2.2. Sample Collection and Preparation

Phytolacca dodecandra was collected from Mbulu district. Botanical identification of the plant species was done at the University of Dar es Salaam, Department of Botany, where voucher specimen were deposited. Plant materials were immediately separated into their component parts (leaves and roots) and placed in the open container in a dark room. The samples were allowed to air dry at room temperature in a dark room with low humidity for about two weeks. The samples were subjected to size reduction where the roots were sliced into pieces of 1 - 2cm long, while leaves were cut into pieces of approximately 1 cm2. All samples were then dried at room temperature for another two weeks for further oven dried at 35°C for 48 hours.

2.3. Sample Extraction

Dry samples were grinded into fine powders using a laboratory electric hammer mill. The powdered plant materials were then stored in air-tight glass jars in a cool place ready for extraction. Extraction of the samples was done using cold method (maceration) where plant powders were extracted using ethanol (95% v/v) for 24 hours at room temperature [26]. Later, the extracts were filtered, concentrated, and then stored in a refrigerator at -4°C for pesticidal activity tests.

2.4. Mass Rearing of the Pests

Selected unsexed adult S. zeamais and T. castaneum species (approximately 250) were introduced into 1-Litre glass jar containing maize grains (500 g) and kept at 25–30°C, 65–70% relative humidity (RH) and 12:12 hours (light: darkness). The jar was covered using plastic stopper reinforced inside with 0.5 mm wire gauze to prevent the insects from chewing through them [6, 27, 28]. The insects were allowed to lay eggs for 14 days. The beetles and flour frass were separated from the treated grains by repeated gentle sieving through layering of 3 mm and 1 mm mesh sieves, respectively. The grains were retained by the 3 mm sieve, then the beetles were retained by 1 mm sieve and the flour frass were retained in the holding pan. The insects which did not come out during the sieving were forced out by probing with a plastic fibber. The grains and flour frass were then returned into the jar and kept at 65–70% RH until the adults emerged.

2.5. Bioactivity Tests against Storage Pests

The activity of plant extracts against selected storage pests were conducted using contact toxicity, repellence, feeding deterrence and first filial generation (F1) progeny studies as described in the following subsections.


2.5.1. Contact Toxicity and First Filial Generation (F1) Progeny Studies

Maize (40 grains) were weighed into glass jars (250 mL) and admixed with plant extracts at seven different doses (0, 50, 100, 150, 200, 250 and 300 mg/mL) in triplicate. One batch of grains was treated with actellic gold TM 2% dust (0.05% w/w) as positive control. Procedure of assessing contact toxicity was as described in [28] and [29]. Briefly, twenty (20) unsexed adult beetles, S. zeamais and T. castaneum (5–10 days old) were placed into each experimental jar in a controlled randomised design (CRD) in triplicate. The top of each jar was covered using plastic stoppers reinforced on the inside with 0.5 mm wire gauze to prevent the insects from chewing through them. The experimental units were kept at 25–30°C and 65–70% R.H. The number of dead (ND) insects in each jar was recorded at 1, 3, 5, 7, 14 and 21 days after treatment (DAT). The adult S. zeamais and T. castaneum were removed from the grains in the experimental jars at 21 DAT and the grains were returned into the jar and kept for F1 progeny counts. The numbers of the newly emerged adults F1 progeny insects were recorded at 28, 35 and 42 DAT. The percentages reduction in adult emergence or reproduction inhibition rate (IR %) was computed as:

(1)

where CN = number of newly emerged adult insects in the un-treated grains and TN = number of newly emerged adult insects in the treated grains.


2.5.2. Feeding Deterrence (Grain Damage) Studies

Maize (40 grains) were weighed into glass jars (250 mL) and admixed with plant extracts at seven different dosage (0, 50, 100, 150, 200, 250 and 300 mg/mL) in triplicate. Each set of experiment were kept at 25–30°C and 65–70% RH as described in [27, 29] and [30]. Here, twenty (20) unsexed adult insects S. zeamais and T. castaneum (5 –10 days old) were introduced into the treated grains and allowed to feed. After 7 and 21 days, insects were removed and the amount of frass (flour) produced were determined by sieving the samples and weighing the resultant frass (flour). Percentage grain damage was computed using the formula:

(2)

where U = Weight of undamaged grains, D = Weight of damaged grains, Nu = number of undamaged grains and Nd = number of damaged grains.


2.5.3. Repellence Studies

The repellent activity of the plant extracts against S. zeamais and T. castaneum was assessed using a locally made Y-shape olfactometer. The test sample extracts dissolved in dimethyl sulfoxide (DMSO) were applied onto a filter paper disc (Whatman No. 1, 1.8 cm diameter). DMSO alone was applied on similar filter paper disc as control. The solvent was allowed to evaporate and the treated and control discs were placed in either arms of the olfactometer.

Randomly selected adults (30 pests) of mixed sex and age were introduced into the olfactometer in triplicate. Prior to the introduction of the test material, air suction were applied at the Y junction of the olfactometer by using an aspirator pump to ensure that it did not become saturated with the test materials which are confined on the treated arm.

All bioassays were conducted using seven rates (0, 50, 100, 150, 200, 250 and 300 mg/mL) in triplicate and kept at 25–30°C, 65–70% R.H. Actellic goldTM dust (2%) was used as a positive control. The same procedure used for extracts was applied for the control. All experiments were performed in a dark room [31]. The assays were left to run for 30 min and after that the number of insects in the control arm (NC) and that in the treated arm (NT) were counted. After each test, the olfactometers were thoroughly cleaned and dried. Percentage repellence (PR) values were computed according to [32]:

(3)

A positive PR value indicated repellence of the insect pests against pesticidal plants, while a negative PR value indicated attracting ability.

2.6. Data Analysis

Data for pesticidal activity were calculated for mean values and analyses using Statistical Package for Social Sciences (IBM SPSS) version 20 where one-way analysis of variance (ANOVA) was used. The resulting data were later presented in form of graphs as described below.

3. Results and Discussion

3.1. Contact Toxicity of P. dodecandra Extracts

The leaf extracts of P. dodecandra at 150 mg/mL concentration killed 98% of S. zeamais and 99% of T. castaenum at day 3, respectively (Figure 2: A and B). The same concentration of P. dodecandra root extract killed only 5% of S. zeamais and 67% of T. castaneum at day 3 (Figure 3: A and B). No mortality was recorded from the untreated control maize, whereas maize treated with actellic goldTM dust achieved 100% mortality within one day. Generally, the percentage mortality increased significantly with the increase in concentration and duration of contact with treated maize (p < 0.05).

Figure 2. Percent Mortality of S. zeamais (A) and T. castaneum (B) using P. dodecandra Leaf Extracts
Figure 3. Percent Mortality of S. zeamais (A) and T. castaneum (B) using P. dodecandra Root Extract

The leaf extracts of P. dodecandra shows relative higher toxicity compared to P. dodecandra roots against adult S. zeamais and T. castaneum. There were a significance difference in the toxicity between leaves and roots extract in which the leaf extracts were more active than root extracts. Leaf extract of P. dodecandra at concentration range of 150 to 300 mg/mL caused 100% mortality of adult S. zeamais and T. castaneum on 3 DAT (Figure 2: A and B). On the other hand, root extracts of P. dodecandra at concentration range of 250 to 300 mg/mL caused mortality between 80% to 81% of S. zeamais and between 80% to 86% mortality of T. castaneum on 21 DAT. (Figure 3: A and B). The powders of dry leaves of P. dodecandra were reported to be used in treating grain storage insects by subsistence farmers around Lake Victoria basin [2]. Some studies have reported high toxicities of plant crude powders from which eight plant species caused mortality between 58% and 88% of S. Zeamais [33]. This toxicity of the plant species supports the current study.

It is evident that P. dodecandra leaf extract is a potential protectant against S. zeamais and T. castaneum. This bioactivity could be associated with the presence of phytochemicals such as saponin, alkaloid, sterol, triterpenoids, phenol, flavanoid and glycocide [14]. The broad spectrum bioactivity of botanical extract in relation to local availability and simple processing make the pesticidal plant acceptable and cost effective compared to synthetic pesticides in small scale agriculture. Therefore, this plant - based products hold good promises for inclusion in the Integrated Pest Management (IPM) strategies.

3.2. Feeding Deterrence of P. dodecandra

Both leaf and root extracts of P. dodecandra exhibited significant feeding deterrence to the test pests (p < 0.05). P. dodecandra leaf extracts at 150 mg/mL concentration and above deterred S. zeamais and T. castaneum for damaging maize grains from day 7 to day 21 similar to Actellic goldTM (Figure 4: A and B ).

Figure 4. Percent Damage of S. zeamais (A) and T. castaneum (B) Pests in Maize applied with P. dodecandra Leaf Extracts

In the untreated samples, there was up to 10% insect damage by day 21 which was significant (p < 0.05). Root extracts of P. dodecandra had little feeding deterrence compared to leaf extracts at the concentration of 150 mg/mL (Figure 5: A and B). This shows that feeding deterrence was inversely proportional to grain damage (Figure 5).

Figure 5. Percent Damage of S. zeamais (A) and T. castaneum (B) Pests in Maize applied with P. dodecandra root extracts

The leaf extract of P. dodecandra shows relatively higher deterrence (less grain damage) to both S. zeamais and T. castaneum than the root extract. Furthermore, S. zeamais seems to be more destructive than T. Castaneum though it responded fast to the leaf extract of P. dodecandra (Figures 5 and 6). There was high grain damage noted in root extracts than the leaf. However, the grain damage decreased with increasing concentration of both leaf and root extracts. Untreated samples had the highest damage and weight frass percentage in all cases.

The antifeedant property of any plant material depends on the active constituents in the plant such as terpenoids, iridoids, glycocides which may be responsible for observed insecticidal properties [34]. Previous studies reported the presence of anti-feedant compound in Azadirachita indica, Tephrosia vogelli, and Lantana camara [4, 6, 35, 36]. For example, leaves and seeds of T. vogelli contain antifeeding deterrents [37, 38, 39]. The fact that P. dodecandra has considerably reduced the damage caused by the adult S. zeamais and T. castaneum, the observed grains protection by the crude extracts could partly be attributed to a modification of the physical properties of stored grains that reduce inter-granular air spaces. This discourages insects’ penetration, feeding and amount of oxygen available. As a result, the crude extracts succeeded in inhibiting insect feeding and oviposition. As a result, the adult insects mortality recorded in the study may largely be due to starvation. Hence, the antifeedant property of test botanicals may be attributed by their bioactive constituents [4]. These findings are promising for their adoption and rationalization for use in subsistence agriculture.

3.3. Repellence Activities of P. dodecandra

P. dodecandra leaf extract demonstrated a moderate repellence ranging from 15.85% at 50 mg/mL to 57% at 300 mg/mL for S. zeamais and from 20.99% at 50 mg/ mL to 66.67% at 300 mg/ mL for T. castaneum (Figure 6A). The repellence activity of P. dodecandra leaf extract at 150 mg/ mL was 75% of the activity of actellic goldTM (standard) against S. zeamais. Similarly, the repellence of T. castaneum was 88% compared to actellic goldTM (standard). The repellence of the pests against the crude extracts of P. dodecandra (Figure 6) concur with the observations indicated by the deterrence experiments (Figure 5).

Figure 6. Mean Percent Repellence of Tested Pests against P. dodecandra leaf (A) and root (B) extracts

Unlike leaves, P. dodecandra root extracts indicated a weak attractance ranging from 5.11% at 50 mg/mL to 44.43% at 300 mg/mL for S. zeamais and from 20.99% at 50 mg/mL to 56.18% at 300 mg/mL for T. castaneum (Figure 6B). In contrast, actellic goldTM had repellence percent of 40.67% and 37.68% for S. zeamais and T. castaneum, respectively. The results of the repellence and attractance bioassay show significant difference between the untreated control and treated samples (p < 0.05). The percentage repellence (PR) values for all insects’ treatment increased significantly with increasing concentration.

It has been reported that the essential oil from plants containing sulphur and saponin compounds contribute to the repellence potency [40]. For example, Ephestia cautella, L. camara and T. vogelli are good repellents to major storage pests [28, 41, 42]. Similarly, reference [43] reported that neem oil repelled various insects including moths. Leaf extracts of P. dodecandra demonstrated significant repellence against S. zeamais and T. castaneum. The observed repellent activity could partly be attributed to the presence of volatile constituents such as monoterpenes and sesquiterpenes which are well known repellents of phytophagous (biting) insects [14, 44, 45]. In addition, aromatic compounds from plants have long been used in daily life as insect repellent because they are generally regarded as safe, economically and socially friendly compared to synthetic pesticides [4]. The repellent effect of P. dodecandra leaves may have important implications on post harvest protection system, since they facilitate maize weevils to depart from the treated grains [31]. The repellent action of P. dodecandra leaves observed is crucial for the traditional poor farming communities in Sub-Saharan Africa whose synthetic pest control is expensive and uneconomical [28].

3.4. First Filial Generation Results

The results of adult F1 progeny counts for S. zeamais and T. castaneum were presented in Figures 7: A and B. The P. dodecandra leaf and root extracts have reduced F1 progeny emergence. Crude extract of P. dodecandra leaves have shown 100% reduction of F1 progeny in both S. zeamais and T. castaneum at 150 mg/mL. The P. dodecandra leaf extract showed high reduction of F1 progeny in S. zeamais and T. castaneum compared to root extract. Indeed, all P. dodecandra botanical treatments indicate significant reduction in F1 progeny emergence compared to the untreated control. The reduction of F1 progeny emergence indicated by P. dodecandra leaf extract was similar to actellic goldTM (positive control) as indicated in Figure 7A and Figure 7B.

Figure 7. First Filial Generations of S. zeamais and T. castaneum Pests in Maize applied with P. dodecandra leaf (A) and root (B) Extracts

The emergence of filial generation progeny decreased as concentration of P. dodecandra increased. Effective botanicals to various insect pests at different dosages have been reported by [1, 46]. There was no significant difference in F1 progeny counts in maize grains treated with crude root extract and that of crude leaf extract (P > 0.05) for both S. zeamais and T. castaneum. The results have shown a clear dose-dependent reduction in the adults S. zeamais and T. castaneum F1 progeny count by P. dodecandra leaf extract at concentrations between 150 to 300 mg/mL and at concentration of 300mg/mL for root extract. The leaf extracts with high concentration reduced F1 progeny count by 100% (similar to actellic goldTM dust at 0.02% w/w), while root extracts reduced F1 progeny count by 93.3% compared to the untreated control (Figure 7).

The reduction of F1 progeny emergence in the treated grains might be due to increased adult mortality, ovicidal and larvicidal properties of the tested P. dodecandra leaf and root extracts. This is in line with the findings observed in [29] that dry leaves of plants inhibited oviposition and subsequent reduction of progeny production of pests. The botanical pesticide probably weakens adult pests by making them lay fewer eggs than normal leading to reduced hatchability of larvae and hence reduced final metamorphosis to adults. The findings reported in this study are similar to those reported by [4]. In that study, maize grains treated with L. camara, T. vegolii and A. indica powder produced 71.6%, 69.7% and 85.6% of F1 progeny reduction, respectively.

4. Conclusion and Recommendations

The findings have shown that P. dodecandra leaf extracts are potential protectants of stored agricultural products against the larger grain borer, S. zeamais (Motsch.) and T. castaneum pests. The broad-spectrum bioactivity of these botanical extracts coupled with their local availability and simple processing method makes them more acceptable and cost-effective alternatives to synthetic pesticides in smallholder agriculture. Hence, these plant-based products hold good promise for inclusion in IPM strategies especially where the emphasis is on ecological preservation and food safety.

It is recommended that biosafety studies should be carried out to confirm whether maize treated with the botanical pesticide can be safely consumed before promoted to farmers. Apparently, depletion of natural forests has called for isolation of important bioactive natural products and identification of their structures. This will later help in making the appropriate naturally based synthetic pesticides.

Competing Interests

The authors declare that they have no competing interests.

List of Abbreviations

CRD = completely randomised design;

IPM = integrated pest management;

OUT = Open University of Tanzania;

ITM = Institute for Traditional Medicines;

F1 = first filial generation;

DAT = days after treatment;

DMSO = dimethyl sulfoxide;

SPSS = Statistical Package for Social Sciences;

ANOVA = analysis of variance

Acknowledgements

The authors would like to thank the Tanzania Commission for Science and Technology (COSTECH) for financial support to project No. CST/SC.327/952/3/2013. Authors are also grateful to the Departments of Medicinal Chemistry and Microbiology, School of Pharmacy, Muhimbili University of Health and Allied Science (MUHAS), for providing the laboratory facilities used in this study.

References

[1]  Adedire, C. O., Obembe, O. O., Akinkurolele, R. O. & Oduleye, O, Responses of Callosobruchus maculates (Coleoptera: Chrysomellidae: Bruchinae) to extracts of cashew kernels, J. Plant Dis. Prot, 118: 75-79. April 2011.
In article      View Article
 
[2]  Mugisha-Kamatenesi, M., Deng, A. L., Ogendo J. O., Omolo, E. O., Mihale, M. J., Otim, M., Buyungo, J. P. & Bett, P. K, Indigenous knowledge of field insect pests and their management around lake Victoria basin in Uganda. African Journal of Environmental Science and Technology. 2 (8): 342-348. October 2008.
In article      
 
[3]  Fèlicia, J; Mauricette, O; Yao, T and Kouahou, F, Specific behaviour of Sitophilus zeamais in the stocks of rice and maize in rural farmers at Bouaflè region, Côte dľoire. International Journal of Agronomy and Agricultural Research. 4(1): 22-31. January 2014.
In article      
 
[4]  Chebet, F., Deng, A. L., Ogendo, J. O., Kamau A. W. and Bett, P. K. Bioactivity of selected plant powders against Prostephanus truncatus (Coleoptera: Bostrichidae) in stored maize grains. Plant Protect. Sci., 49: 34-43. January 2013.
In article      
 
[5]  Gemu, M; Getu, E; Yusuf, A and Tadesse, A, Management of Sitophilus zeamais Motshulsky (Coleoptera: Ciurculionidae) and Sitotroga cerealella (Olivier) (Lepidoptera: Gelechidae) using locally available agricultural wastes in Southern Ethiopia. Journal of Agricultural and Crop Research. 1(1): 10-16. July 2013.
In article      
 
[6]  Ogemah, V. K, Influence of neem products on the biology and behaviour of the larger grain borer Prostephanus truncatus and its predator Teretrius nigrescens. PhD Thesis. Humboldt University, Germany. 2003
In article      
 
[7]  Mihale, M. J., Deng , A. L., Selemani, H. O. Mugisha-Kamatenesi, M., Kidukuli , A. W. and Ogendo, J. O, Use of indigenous knowledge in the management of field and storage pests around Lake Victoria basin in Tanzania. African Journal of Environmental Science and Technology 3 (9): 251-259. September 2009.
In article      
 
[8]  Nkunya, M. H. H, Unusual metabolites from some Tanzanian indigenous plant species. Department of Chemistry, University of Dar es Salaam, Tanzania. IUPAC Pure Appl. Chem. 77(11): 1943-1955. 2005.
In article      View Article
 
[9]  ITM, (2012). Institute of Traditional Medicine. Muhimbili University of Health and Allied Sciences. Available at: http://www.muhas.ac.tz/ITM1/aboutus.htm. (Accessed on 30th Oct 2014).
In article      
 
[10]  Nahashon, M, Conservation of wild-harvested medicinal plants species in Tanzania: Chain and consequence of commercial trade on medicinal plant species. Master Thesis, Uppsala University, Sweden. 2013, No: 124, 50pp.
In article      
 
[11]  FAO. Global forest resources assessment. Country report, United Republic of Tanzania. Forestry department, Food and Agriculture Organisation of the United Nations. FRA 2010 – Country Report, Tanzania. 2010. Rome, Italy.
In article      
 
[12]  Lemma, A. Laboratory and field evaluation of the molluscicidal properties of Phytolacca dodecandra. Bull. Org. Mond. Sante. 42: 597-612. 1970.
In article      
 
[13]  Lemma, A, Molluscicidal and other economic potentials of Endod. Ethiopian Med. J. 12:109-114, 1974.
In article      PubMed
 
[14]  Qwarse, M, Assessment of bioactivity of selected plants against pests and microbes from Agro-pastoral communities in Mbulu district, Tanzania, MSc.Thesis, Open University of Tanzania, Dar es Salaaam. (2015).
In article      
 
[15]  Misganaw, N., Moges, S., Tadele, M., Tesera, M., Temeggen, T and Raja, N, Evaluation of Multi potential bioactive endod, Phytolacca dodecandra (L’herit) berries extracts against immature filarial vector culex quinquefasciatus say (Diptera: culicidae). Research Journal of environmental and Earth Science. 4(7): 697-703. July 2012.
In article      
 
[16]  Karunamoorthi, K., Bishaw, D., and Mulat, T, Laboratory evaluation of Ethiopia local plant Phytolacca dodecandra extract for its toxicity effectiveness against aquatic Macro inverberbrates. European Review for Medicinal and Pharmacological Science. 12: 381-386. November 2008.
In article      PubMed
 
[17]  Khaliq, A; Nawaz, A., Ahmad, M. H and Sagheer, M, Assessment of insecticidal potential of medicinal plant extracts for control of maize weevil, Sitophilus zeamais motschulsky (Coleoptera: Curculionidae). Basic Research Journal of Agricultural Science and Review. 3(11): 100-104. November 2014.
In article      
 
[18]  Asawalam E. F. and L. Onu, L, Evaluation of some plant powders against Khapra beetle (Trogoderma granarium Everts) (Coleoptera: Dermestidae) on stored groundnut. Advancement in Medicinal Plant Research. 2(2): 27-33. May 2014.
In article      
 
[19]  Ileleji, K. E., Maier, D. E. and Woloshuk, Evaluation of different temperature management strategies for suppression of Sitophilus zemais (Motschulsky) in stored. Journal of stored products Research. 43(4): 480-488. December 2007.
In article      View Article
 
[20]  Rees, D, Insects of stored products. CSIRO Publishing, Melbourne, Victoria,. 2004.
In article      
 
[21]  Yuya, A. I; Tadesse, A; Azerefegne, and Tefera, T (2009). Efficacy of combinig Niger seed oil with malathion 5% dust formulation on maize against the maize weevil, Sitophilus zeamais (Coleoptera: Curculionidae). J. Stored. Prod. Res. 45: 67-70. December 2009.
In article      View Article
 
[22]  Chaisaeng, P; Chongrattanameteekul, W; Visarathanonth, P and Vajarasathiara, B, Laboratory studies on control of the maize weevil zeamais by the parasitoid Anisopteromalus calandrae. Science Asia. 36:6-11. January 2010.
In article      View Article
 
[23]  Nishi, A; Imamura, T; Miyanoshita, A; Morimoto, S; Takahashi, K; Visarathanoth, P Kengkanpanich, R; Shazali, M. H and Sato, K, Predatory abilities of amphiboles venator (klug) (Hemiptera: Reduviidae), a predator of stored product insect pest. Appl. Entomol. Zool. 39: 321-326. 2004.
In article      View Article
 
[24]  Red flour beetle, Wikipedia, the free encyclopaedia. Available: at https://en.wikipedia.org/wiki/Red_flour_beetle. [Accessed April 19, 2016].
In article      
 
[25]  Magoggo, J. P and Meliyo, J. L, Land resources inventory and land suitability assessment of Mbulu district, Arusha region, Tanzania NSSR report R5, National soil service. 1994. 75pp.
In article      
 
[26]  Omoregbe, R. E, Ikuebe, O. M , Ihimire, I. G,. Antimicrobial activity of some medicinal plants extracts on Escherichia coli, Salmonella paratyphi and Shigella dysenteriae. African Journal of Medicine and Medical Sciences. 25(4):373-375. December 1996.
In article      PubMed
 
[27]  Ogendo, J. O., Deng, A. L., Belmain, S. R., Walker, D. J. & Musandu, A. A. O, Effect of insecticidal plant materials, Lantana camara L. and Tephrosia vogelii Hook, on the quality parameters of stored maize grains. Journal of Food Technology in Africa. 9 (1): 29-35. March 2004.
In article      
 
[28]  Ogendo, J. O., Belmain, S.R., Deng, A. L. & and Walker, D. L, Efficacy of Lantana camara and Tephrosia vogelli Hook agaist Sitophilus zeamais (Coleoptera: Curculionidae) in stored maize grains. Journal of Food Technology in Africa. 9: 29-35. 2005.
In article      
 
[29]  Tapondjou, L. A., Adler, C., Bouda, H. & Fontem, D. A, Efficacy of powder and essential oil from Chenopodium ambrosioides leaves as post-harvest grain protectants against six-stored product beetles, Journal of Stored Products Research. 38(4): 395-402. 2002.
In article      View Article
 
[30]  Haines, C. P. (ed.), Insects and Arachnids of tropical stored products” their biology and Identification. A training Manual. Natural Resources Institute, UK. 2015. 2nd edition.
In article      
 
[31]  Karemu, C. K., Ndung’u, M. W. & Githua, M, Repellent effects of essential oils from Selected Eucalyptus species and their major constituents against Sitophilus zeamais (Coleoptera: Curculionidae). International Journal of Tropical Insect Science. 33(3): 188-1994. September, 2013
In article      View Article
 
[32]  Ndung’u, M. W, Repellent activity of the essential oils of Cleome monophylla L., C. hirta Oliv., Gynandropsis gynandra Brig. and Capparis tomentosa Lam., and their chemical constituents against Sitophilus zeamais and Rhipicephalus appendiculatus. MSc. Thesis, Kenyatta University, Nairobi, Kenya, pp. 37-46. 1993.
In article      
 
[33]  Kasa, A. and Tadese, A. (1996). Evaluation of some botanicals against the maize weevil, Sitophilus zeamais (Motsch.) on stored sorghum at Bako. p.120-126. In Proc. 3rd Annual Conference of Crop Protection Society of Ethiopia, May 18-19, 1995, Addis Ababa, Ethiopia.
In article      
 
[34]  Sharma, R. K, Neem leaf powder and cop ash against R. dominica in tored maize. Indian Journal of Entomology. 57: 15-17. 1995
In article      
 
[35]  Butterworth, J. H., & Morgan, E. D, Isolation of substance that suppresses feeding in locusts. Chemical Communications (London): 23-24. 1968.
In article      View Article
 
[36]  Rembold, H, Growth and metamorphosis. in The neem tree: Source of unique natural products for integrated pest management, and medicinal, industrial and other purposes. VCH, Weinheim: 418-432. 1995
In article      
 
[37]  Isman, M. B, Plant essential oils for pest and disease management. Crop Prot 19:603-608. September 2000.
In article      View Article
 
[38]  Isman M.B, Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology. 51:45-66. 2006.
In article      View Article  PubMed
 
[39]  Adebayo, T. A., Olaniram, O. A. & Akanbi, W. B, Control of insect pests of cowpea in the field with allelochems from Tephrosia vogelii and Petiveria alliacea in Southern Guinea Savannah of Nigeria. Agricultural Journal. 2: 365-369. 2007
In article      
 
[40]  Ascher, K. R. S, Non-conventional insecticidal effects of pesticides available from the neem tree, Azadirachta indica. Archives of Insect Biochemistry and Physiology. 22: 433-449. 1993.
In article      View Article
 
[41]  Bekele, A. J., Obeng-Ofori, D. & Hassanali, A, Evaluation of Ocimum kenyense (Ayobangira) as source of repellents, toxicants and protectants in storage against three major stored product insect pests. Journal of Applied Entomology. 121: 169-173. January 1997
In article      View Article
 
[42]  Shehu, A., Obeng-Ofori, D. & Eziah, V. Y, Biological efficacy of calneemTM oil against the tropical Warehouse moth Ephestia cautella (Lepidoptera: Pralidae) in stored maize. International Journal of Tropical Insect Science. 30(4): 207-213. December 2010.
In article      View Article
 
[43]  Blum, M. & Roitberg, M. (1999). Natural insect repellent formula and method of making same. New York. United States Patent 5885600. Available: http://www.freepatentsonline.com/5885600.html [Accessed: July 15, 2015).
In article      
 
[44]  Debboun M., Frances S. P., & Strickman, D. (Eds). Insect repellents-principles, methods and uses. CRC Press, Taylor and Francis Group, Boca Raton-London-New York. 2007.
In article      
 
[45]  Wang, Z., Song J., Chen, J., Song Z., Shang, S., Jiang, Z. & Han, Z, QSAR study of mosquito repellents from terpenoid with a six-member-ring. Bioorganic and Medicinal Chemistry Letter. 18: 2854-2859. May 2008.
In article      View Article  PubMed
 
[46]  Fekadui, G., Waktole, S. & Santiago, R. D, Evaluation of plant powders and cooking oils against maize weevil, Sitophilus zeamais M. (Coleopteran: Curculionidae) under laboratory conditions. Molecular Entomology. 3(2): 4-14. 2012.
In article      
 
  • CiteULikeCiteULike
  • MendeleyMendeley
  • StumbleUponStumbleUpon
  • Add to DeliciousDelicious
  • FacebookFacebook
  • TwitterTwitter
  • LinkedInLinkedIn