Figure 5. Effect of CO-EtOH on hepatic cholesterol degradation-related genes and proteins. Sprague-Dawley rats were orally administered CO-EtOH (760 mg/kg body weight) or CMC daily. The expression of CYP7A1 (A) was evaluated by RT-qPCR. The expression of CYP7A1 (B) was detected by western blot. The expression of FXR and SHP (C) was evaluated by RT-qPCR. The expression of FXR (D), and SHP (E) were detected by western blot. The expression of BESP, ABCG5, ABCG8 (F), MRP3, MRP4, and NCTP (G) were evaluated by RT-qPCR. Values are expressed as mean ± SEM (n = 8-10). Statistical significance was analyzed by ANOVA and then by LSD multiple range test. Note: p < 0.05 was considered statistically significant. * indicates statistical difference between DC group and NC group. #indicates statistical difference between DC + CO-EtOH group and DC group : Study on Mechanisms Underlying the Preventive Effects of Canarium album L. ethanol Extract on Modulation of Hyperglycemia and Hypercholesterolemia in Diabetic Rats : Science and Education Publishing

Figure 5. Effect of CO-EtOH on hepatic cholesterol degradation-related genes and proteins. Sprague-Dawley rats were orally administered CO-EtOH (760 mg/kg body weight) or CMC daily. The expression of CYP7A1 (A) was evaluated by RT-qPCR. The expression of CYP7A1 (B) was detected by western blot. The expression of FXR and SHP (C) was evaluated by RT-qPCR. The expression of FXR (D), and SHP (E) were detected by western blot. The expression of BESP, ABCG5, ABCG8 (F), MRP3, MRP4, and NCTP (G) were evaluated by RT-qPCR. Values are expressed as mean ± SEM (n = 8-10). Statistical significance was analyzed by ANOVA and then by LSD multiple range test. Note: p < 0.05 was considered statistically significant. * indicates statistical difference between DC group and NC group. #indicates statistical difference between DC + CO-EtOH group and DC group

Journal of Food and Nutrition Research. 2018, 6(4), 261-270 doi:10.12691/jfnr-6-4-9