Figure 6. S-nitrosylated IKK-β Expression in LPS-induced Caco-2 and HT-29 cells by treated with curcumin. S-nitrosylated IKKβ as obtained from cell lysates of each experimental group at12hindividually by biotin switch method and detected by Western blot using anti-biotin. The expression level of IKKβ was as the control and relative fold change of S-nitrosylated IKKβ was normalized by IKKβ protein expression. The data was repeated 3 times independently to ensure reproducibility and the standard deviation of the mean are represented as error bars (n=3). Values with different letters were significantly different (p< 0.05) at corresponding concentrations between different treatments : Curcumin Suppresses the Activity of Inhibitor-κB Kinase in an in vitro Inflamed Human Intestinal Mucosa Model by S-nitrosylation : Science and Education Publishing

Figure 6. S-nitrosylated IKK-β Expression in LPS-induced Caco-2 and HT-29 cells by treated with curcumin. S-nitrosylated IKKβ as obtained from cell lysates of each experimental group at12hindividually by biotin switch method and detected by Western blot using anti-biotin. The expression level of IKKβ was as the control and relative fold change of S-nitrosylated IKKβ was normalized by IKKβ protein expression. The data was repeated 3 times independently to ensure reproducibility and the standard deviation of the mean are represented as error bars (n=3). Values with different letters were significantly different (p< 0.05) at corresponding concentrations between different treatments

Journal of Food and Nutrition Research. 2017, 5(6), 406-412 doi:10.12691/jfnr-5-6-7