Figure 5. Total S-nitrosylated protein expression in LPS-induced Caco-2 and HT-29 cells by treated with curcumin. The total S-nitrosylated protein extracted from Caco-2 and HT-29 cell lysates of each experimental group at12h individually that was prepared by biotin switch method and detected by Western blot using anti-biotin. The bar-chart presented the relative quantification of total S-nitrosylation from Western blot. The data was repeated 3 times independently to ensure reproducibility and the standard deviation of the mean are represented as error bars (n=3). Values with different letters were significantly different (p< 0.05) at corresponding concentrations between different treatments

From

Curcumin Suppresses the Activity of Inhibitor-κB Kinase in an in vitro Inflamed Human Intestinal Mucosa Model by S-nitrosylation

Ning-Jo Kao, Zwe-Ling Kong

Journal of Food and Nutrition Research. 2017, 5(6), 406-412 doi:10.12691/jfnr-5-6-7