Figure 3. Effect of Adzuki extract on expression of cell cycle-related molecules in 3T3-L1 adipocytes. (A) Adzuki extract or a vehicle was added to the differentiation medium at day 0 and cells were cultured for 24 h. The mRNA expression of Ccnd1 was determined by real-time PCR and normalized by GAPDH. The results are expressed as means ± SEM for three independent determinations. ** P<0.01 compared with control. (B) Immunoblotting of 3T3-L1 cell lysates with phosphor Rb polyclonal antibody. Adzuki extract or a vehicle was added to the differentiation medium at day 0 and cells were cultured for 24 h or 72h. The results are representative of three identical experiments

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Adzuki Bean (Vigna angularis) Extract Inhibits 3T3-L1 Preadipocyte Differentiation

Shinichi Yonekura, Yoshihito Obayashi, Yukako Tokutake, Sei-ichi Kawahara, Hidefumi Makabe, Hiroshi Fujii

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