Figure 2. Effect of Adzuki extract on lipid accumulation in 3T3-L1 adipocytes. (A) Adzuki extract or a vehicle was added to the differentiation medium at day 0 and cells were cultured for 8 day. Cells were stained with Oil Red O and observed under the microscope. (B) Oil Red O-stained intracellular lipids were then extracted using isopropanol and quantified by measuring the optical density at 490 nm. The results are expressed as means ± SEM for three independent determinations. *P<0.05 compared with control. (C) Undifferentiated 3T3-L1 cells were treated with Azuki extract or a vehide. Cell viability was measured by the MTT assay after 72 hr. The results are expressed as means ± SEM for three independent determinations

From

Adzuki Bean (Vigna angularis) Extract Inhibits 3T3-L1 Preadipocyte Differentiation

Shinichi Yonekura, Yoshihito Obayashi, Yukako Tokutake, Sei-ichi Kawahara, Hidefumi Makabe, Hiroshi Fujii

Journal of Food and Nutrition Research. 2017, 5(12), 908-913 doi:10.12691/jfnr-5-12-5