Figure 6. Calcium binding activity of BBP. Various concentrations of BBP (up to 500 mg/l) were mixed with 5 mM CaCl₂ and 20 mM sodium phosphate buffer (pH 7.5). The mixture was stirred at 25oC for 30 min, pH 7.5. After with 0.22 μm microfiltration to remove insoluble calcium phosphate salts, calcium contents of the supernatant were determined by FAAS (AA-6300C, Shimadzu Co., Japan). The experiments were performed in triplicate, values are expressed as means ± SD

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Purification of Bovine Bone Oligophosphopeptide with High Calcium-binding Activity by Bacillus cereus MBL13 Collagenolytic Protease

Lili Liu, Chenliu Yang, Yang Zhu, Yuanyuan Meng, Xiaoning Dai

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