Figure 2. Effect of demineralized on enzymatic hydrolysis of bovine bone. (a) Effect of demineralization on degree of hydrolysis of bovine bone powder; (b) calcium-binding activity of bovine bone with different degrees of demineralization. DM0, bovine bone without demineralization. DM1, DM2, DM3, and DM4 represent four kinds of demineralized bovine bone with demineralization degrees of 16.12%, 33.07%, 50.78%, and 71.01% respectively. Bovine bone was digested with BCC (enzyme/substrate: 3/100, substrate concentration: 2%, pH 8.0) at 45°C for 6.0 h. The assay was performed in triplicate; values are expressed as means± SD

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Purification of Bovine Bone Oligophosphopeptide with High Calcium-binding Activity by Bacillus cereus MBL13 Collagenolytic Protease

Lili Liu, Chenliu Yang, Yang Zhu, Yuanyuan Meng, Xiaoning Dai

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