Figure 4. Tricine-SDS-PAGE electrophoresis of BBP obtained from bovine bone hydrolysates, with 10% polyacrylamide resolving gels. Lane 1: BBP isolated from bovine bone, lane 2: molecular mass markers (20.1, 14.4, 7.8, 5.8, and 3.3 kDa). Protein was visualized by Commassie Brilliant Blue R 250 staining of the gel. The picture is a representative of three similar experiments

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Purification of Bovine Bone Oligophosphopeptide with High Calcium-binding Activity by Bacillus cereus MBL13 Collagenolytic Protease

Lili Liu, Chenliu Yang, Yang Zhu, Yuanyuan Meng, Xiaoning Dai

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