Figure 4. Silibinin inhibits the expression of MAPKs express in LPS-induced RAW264.7 cells. (A) RAW264.7 cells were pre-treated with indicated concentration of silibinin for 4 hours, and then co-treat with LPS for 30 min. Western blot were conducted by incubating total lysate were with p-p38MAPK, p38MAPK, p-ERK, ERK, p-JNK, and JNK specific antibodies. (B) p-ERK protein was proved for antibodies against to p-ERK and ERK as a loading control

From

Silibinin Suppresses Mediators of Inflammation through the Inhibition of TLR4-TAK1 Pathway in LPS-induced RAW264.7 Cells

Ji-Hyeon Song, Kui-Jin Kim, Boo-Yong Lee

Journal of Food and Nutrition Research. 2016, 4(8), 515-521 doi:10.12691/jfnr-4-8-5