Figure 9. Effects of LCA on the level of HIF-1α Transcript levels for HIF-1α was monitored by RT-PCR analysis and relative intensities were normalized by levels of GAPDH. LCA-untreated group level was accepted to be “1.0” (A). Protein levels for HIF-1α was monitored by Western blot analysis and Quantitative of protein level were adjusted with the internal control β-actin protein level (B). Data are presented as means ± SD from three independent experiments. * P< 0.05, ** P< 0.01 as compared with the LCA-untreated control group


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