Figure 6. Gelidium elegans extract stimulates a beige cell-like phenotype and increases the production of carbon dioxide in 3T3-L1 cells. Fully confluent pre-adipocytes were induced by MDI to differentiate into mature adipocytes in the presence or absence of Gelidium elegans extract (GE) or hesperidin (HP) for 8 days. Total protein was harvested after 8 days, and each parameter was measured using western blot analysis with specific antibodies. The protein expression levels were normalized against GAPDH. (A) Representative western blot of Lipin1, PRDM16, and UCP1 in 3T3-L1 cells. (B) The production of carbon dioxide (CO2) was used to assess adipocyte energy expenditure at 8 days in the presence or absence of GE. These data were measured as the standard deviation of triplicates. Values with different letters are significantly different, p<0.05

From

Altered Gelidium elegans Extract-stimulated Beige-like Phenotype Attenuates Adipogenesis in 3T3-L1 Cells

Jia Choi, Kui-Jin Kim, Eun-Jeong Koh, Boo-Yong Lee

Journal of Food and Nutrition Research. 2016, 4(7), 448-453 doi:10.12691/jfnr-4-7-6