Figure 4. Gelidium elegans (GE) discourages increased triglyceride content via inhibition of triglyceride synthesis enzymes in 3T3-L1 cells. Fully confluent pre-adipocytes were induced by MDI to differentiate into mature adipocytes in the presence or absence of Gelidium elegans extract (GE) or hesperidin (HP) for 8 days. Total protein was harvested after 8 days, and the triglyceride (TG) content was measured using a triglyceride content assay kit, as described in the Materials and Methods, and with western blot analysis with specific antibodies. (A) The level of TG in 3T3-L1 cells. These data were measured as the standard deviation of quadruplicates. Values with different letters are significantly different, p<0.05. (B) The protein expression levels were normalized against GAPDH. Representative western blot of triglyceride synthesis enzymes including SREBP-1, LPAATθ, and DGAT1 in 3T3-L1 cells


Altered Gelidium elegans Extract-stimulated Beige-like Phenotype Attenuates Adipogenesis in 3T3-L1 Cells

Jia Choi, Kui-Jin Kim, Eun-Jeong Koh, Boo-Yong Lee

Journal of Food and Nutrition Research. 2016, 4(7), 448-453 doi:10.12691/jfnr-4-7-6