Figure 5. The transcription levels of pro-inflammatory cytokines were down-regulated by brazilin in LPS-induced Raw264.7. Cells were pretreated with 10, 30, or 50 μM brazilein for 1 h, stimulated with LPS (1 μg/mL), and incubated for a further 24 h. DMSO was used as a vehicle. Data are expressed as mean ± SD (n=3). (A) Total RNA was amplified by RT-PCR using the indicated primers for IL-1β, TNFα, and IL-6. (B-D) The relative mRNA expression of IL-1β, TNFα, and IL-6.were quantified using the ImageJ program. Data are mean ± SD (n=3)

From

Brazilin Suppresses Inflammation via the Down-regulation of IRAK4 in LPS-stimulated Raw264.7 Macrophage

Kye-Yoon Yoon, Kui-Jin Kim, Hyung-Sun Youn, Sei-Ryang Oh, Boo-Yong Lee

Journal of Food and Nutrition Research. 2015, 3(9), 575-580 doi:10.12691/jfnr-3-9-3