Figure 4. LPS increased the expression of IRAK4 protein, whereas brazilin diminished the expression of IRAK4 in Raw264.7 macrophage cell. Cells were pretreated with 10, 30 or 50 μM brazilein for 1 h and then stimulated with 1 μg/mL LPS for 30 min or 24 h. DMSO was used as a vehicle. Equal amounts of protein in cell lysates were analyzed by Western blot


Brazilin Suppresses Inflammation via the Down-regulation of IRAK4 in LPS-stimulated Raw264.7 Macrophage

Kye-Yoon Yoon, Kui-Jin Kim, Hyung-Sun Youn, Sei-Ryang Oh, Boo-Yong Lee

Journal of Food and Nutrition Research. 2015, 3(9), 575-580 doi:10.12691/jfnr-3-9-3