Figure 3. Brazilin decreased the nitric oxide production and phosphorylation of JNK in LPS-stimulated Raw264.7 macrophage cell. (A) The production of soluble nitrite oxide. Culture media was subsequently isolated and nitrite concentrations determined. (B) The expression of p-JNK, p-p38MAPK, pERK, and ERK in LPS-stimulated Raw264.7 macrophage cell. Cells were pretreated with 10, 30 or 50 μM brazilin for 1 h and then stimulated with 1 μg/mL LPS for 30 min or 24 h. DMSO was used as a vehicle. Equal amounts of protein in cell lysates were analyzed by Western blot. Data are mean ± SD (n=3)

From

Brazilin Suppresses Inflammation via the Down-regulation of IRAK4 in LPS-stimulated Raw264.7 Macrophage

Kye-Yoon Yoon, Kui-Jin Kim, Hyung-Sun Youn, Sei-Ryang Oh, Boo-Yong Lee

Journal of Food and Nutrition Research. 2015, 3(9), 575-580 doi:10.12691/jfnr-3-9-3