Figure 2. Brazilin down-regulated the expression of iNOS and COX2 protein in LPS stimulated Raw264.7. Raw 264.7 macrophage cells (5×105 /well) were pretreated with 10, 30, or 50 μM brazilin for 1 h and then induced with 1 μg/mL LPS for an 24 h. The expression of iNOS and COX2 protein were detected by western blotting using iNOS, COX2, and GAPDH antibodies. DMSO was used as a vehicle. Data are expressed as mean ± SD (n=3)

From

Brazilin Suppresses Inflammation via the Down-regulation of IRAK4 in LPS-stimulated Raw264.7 Macrophage

Kye-Yoon Yoon, Kui-Jin Kim, Hyung-Sun Youn, Sei-Ryang Oh, Boo-Yong Lee

Journal of Food and Nutrition Research. 2015, 3(9), 575-580 doi:10.12691/jfnr-3-9-3