1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China
2Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan, China
3Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China
4College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China
The main purpose of this study is to determine the effect of combined procyanidins extracted from the lotus seedpod (LSPC) and N-acetyl cysteine (NAC) on the memory impairment induced by scopolamine (3 mg/kg, i.p.) in mice. The capacities of memory and learning were evaluated by Y-maze test after 20-day administration of NAC (90 mg/kg BW), LSPC (60 mg/kg BW), and combined LSPC and NAC (60 mg/kg BW + 90 mg/kg BW), respectively. It was indicated that LSPC and combined LSPC and NAC significantly reduced the number of incorrect responses compared with that of scopolamine in Y-maze test. In addition, combination of LSPC and NAC was also found to enhance total antioxidant capacity (T-AOC) level, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in brain and serum, and inhibit acetyl cholinesterase (AchE) activity in brain and neural nitric oxide synthase (nNOS) activities as well as nNOS relative mRNA level. To be mentioned, the T-AOC level in brain of LSPC+NAC group increased by 32.46% and 36.04% relative to those of NAC group and LSPC group, respectively, and the GPx activity increased by 45.79% and 16.17%, while the nNOS activity decreased by 38.24% and 36.36%. These results demonstrate that combination of LSPC and NAC exhibited better ameliorative effects in scopolamine-induced memory impairment mice than treated by NAC and LSPC alone, which suggest LSPC and NAC combination may provide a viable therapy in the treatment for Alzheimer’s disease and other forms of cognitive impairment diseases.
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