Stevia rebaudian Bertoni, belonging to the Compositae family, is a sweet herb contains diterpene glycosides, namely, stevioside, rebaudiosides A–F, steviolbioside, and dulcoside A, which are responsible for the typical sweet taste. Stevia (Stevia rebaudian Bertoni) leaves were analyzed for their physiochemical properties, chemical composition and microbiological contamination in addition to rebauidioside A productively. The carbohydrates content was 63.10%, while the moisture, fiber, protein, ash, fat and reducing sugar contents were 10.73%, 5.03%,13.68%,12.06%,6.13% and 4.50%, respectively. Anti-nutritional value (tannin) was 5.43 %. The total soluble substance was 17.03%. The Stevia leaves showed a good antimicrobial agent for all tested bacterial groups including Coliforms, Staphylococcus. Rebauidioside A was extracted and purified from the dried Stevia leaves by ultrasonic-assisted extraction method. The optimum extraction time was 6 min in which the maximum rebauidioside A content was obtained (32.79 g/100 g). Results show that particle size of sample treated for 6 min is bigger than those for 12, 18 and 24 min. Application of ultrasound-assisted extraction on the Stevia plant substantially affects the Color, and particle size of the stevia leaf extract and rebauidioside A yield.
| [1] | Puri M., Sharma D., Tiwari AK., “Downstream processing of stevioside and its potential Applications,” Biotech adv, 29. 781-79. 2011.View Article PubMed |
| [2] | DuBois GE., Stephenson RA., “Diterpenoid sweeteners. Synthesis and sensory evaluation of stevioside analogs with improved organoleptic properties,” J Med Chem, 28. 93-98. 1985.View Article PubMed |
| [3] | Wang, L., Weller, CL., “Recent advances in extraction of nutraceuticals from plants,” Trends Food Sci Tech, 17. 300-312. 2006.View Article |
| [4] | Kim, J., Choi, Y.H., Choi, Y-H: Use of stevioside and cultivation of Stevia rebaudiana in Korea, A. Douglas Kinghorn, 2002, 196 |
| [5] | Mizutani, K., Tanaka, O., “Use of Stevia rebaudiana sweeteners in Japan, Stevia, the Genus Stevia,” Med Aromat Plants, 19. 178-195. 2002. |
| [6] | Shetty, P., “Public health: India's diabetes time bomb,” Nature, 48. 514-516. 2012.View Article |
| [7] | Geuns, J., “Stevioside,” Phytochemistry, 64. 913-921. 2003.View Article |
| [8] | Fronza, D., Folegatti, M.V., “Water consumption of the stevia (Stevia rebaudiana (Bert.) Bertoni) crop estimated through microlysimeter,” Sci Agr, 60, 595-599. 2003.View Article |
| [9] | Rahi, P., Pathania, V., Gulati, A., Singh, B., Bhanwra, R.K., Tewari, R., “Stimulatory effect of phosphate- solubilizing bacteria on plant growth, stevioside and rebaudioside-A contents of Stevia rebaudiana Bertoni,” App Soil Ecol, 46. 222-229.2010. |
| [10] | Madan, S., Ahmad, S., Singh, G., Kohli, K., Kumar, Y., Singh, R., Garg, M., “Stevia rebaudiana (Bert.) Bertoni - a review,” Indian J Nat Prod Resour, 1. 267-286. 2010. |
| [11] | Das, K., Dang, R., Shivananda, T., “Influence of bio-fertilizers on the availability of nutrients (N, P and K) in soil in relation to growth and yield of Stevia rebaudiana grown in South India,” Int J Appl Res Nat Prod, 1. 20-24. 2008. |
| [12] | Nabors, L.O.B., Alternative sweeteners, 2012. CRC Press |
| [13] | Mason, T., Power ultrasound in food processing—the way, Ultrasound in food processing, 1998, 105 |
| [14] | Mulet, A., Benedito, J., Golás, Y., Cárcel, J.A., “Noninvasive ultrasonic measurements in the food industry,” Food Rev Int, 18. 123-133. 2002.View Article |
| [15] | Mason, T., Paniwnyk, L., Lorimer, J., “The uses of ultrasound in food technology,” Ultrason Sonochem, 3. 253-260. 1996.View Article |
| [16] | Condón, S., Raso, J., Pagán, R., Barbosa-Cánovas, G., Tapia, M., Cano, M., “Microbial inactivation by ultrasound,” Novel food processing technologies, 423-442. 2005. |
| [17] | Neppiras, E., “Acoustic cavitation series: part one: Acoustic cavitation: an introduction,” Ultrasonics, 22. 25-28. 1984.View Article |
| [18] | Henglein, A., “Sonochemistry: historical developments and modern aspects,” Ultrasonics, 25. 6-16.1987. |
| [19] | Gasmalla, M.A.A., Yang, R.A., Amadou, I., Hua, X., “Nutritional composition of Stevia rebaudiana Bertoni leaf: Effect of drying method,” Trop J Pharm Res, 13. 61-65.2014. |
| [20] | Association of Official Analytical Chemists International. Official Methods of Analysis of the Association of Official Analytical Chemists International 17th edn., Arlington, VA, USA, 2000. |
| [21] | ICUMSA (International Commission Uniform Methods of Sugar Analysis). Method book1st supplement. (1998). |
| [22] | Kimball, D.A., Citrus processing: a complete guide, Aspen Gaithersburg, MD, 1999, 450.View Article |
| [23] | Buera, M., Lozano, R., Petriella, C., “Definition of colour in the non-enzymatic browning Process,” Die Farbe, 32. 318-322.1986. |
| [24] | Guerrero, S., Alzamora, S., Gerschenson, L., “Optimization of a combined factors technology for preserving banana purée to minimize colour changes using the response surface methodology,” J food eng, 28. 307-322.1996. |
| [25] | Castañón, X., Argaiz, A., Lopez-Malo, A., “Effect of storage temperature on the microbial and color stability of banana purée with addition of vanillin or potassium sorbate/Efecto de la temperatura de almacenamiento en la estabilidad microbiológica y en el color del puré de plátano con la adición de vainillina o sorbato de potasio,” Food Sci Technol Int, 5. 51-58.1999. |
| [26] | Mishra, P., Singh, R., Kumar, U., Prakash, V., “Stevia rebaudiana - A magical sweetener,” Global J Biotechnol Biochem, 5. 62-74. 2010. |
| [27] | Tadhani, M., Subhash, R., “Preliminary Studies on Stevia rebaudiana Leaves: Proximal Composition, Mineral Analysis and Phytochemical Screening,” J Medical Sci, 6. 321-326. 2006.View Article |
| [28] | Rai, C., Majumdar, G.C., De, S., “Primary Clarification of Stevia Extract: A Comparison Between Centrifugation and Microfiltration,” Separ Sci Technol, 48. 113-121. 2013.View Article |
| [29] | Kaushik, R., Pradeep, N., Vamshi, V., Geetha, M., Usha, A., “Nutrient composition of cultivated Stevia leaves and the influence of polyphenols and plant pigments on sensory and antioxidant properties of leaf extracts,” J Food Sci Technol. 47. 27-33. 2010.View Article PubMed PubMed |
| [30] | Esmat Abou-Arab, A., Azza Abou-Arab, A., Ferial Abu-Salem, M.,“Physico-chemical assessment of natural sweeteners steviosides produced from Stevia rebaudiana bertoni plant,” African J Food Sci, 4. 269-281. 2010. |
| [31] | Serio, L., “La Stévia, une alternative au sucre et à l'aspartame,” Phytothérapie, 8. 26-32. 2010.View Article |
| [32] | Gisleine, E.C, Abdol, H.A, Caudio, C.A., Letícia de, A.F.F., Gilson, T., Mirian, H.T.,Wilson, E.F., Roberto, B.B., “Investigation of the tolerability of oral Stevioside in Brazilian hyperlipidemic Patients,” Brazilian Arch Biol Technol,” 49. 583-587. 2006. |
| [33] | Atanassova M. Christova-Bagdassarian V. Determination of Tannins Content byTitrimetric Method for Comparison of Different Plant Species, Journal of the University of Chemical Technology and Metallurgy.2009; 44: 413-415. |
| [34] | Genovese, D., Elustondo, M., Lozano, J., “Color and cloud stabilization in cloudy apple juice by steam heating during crushing,” J Food Sci, 62. 1171-1175. 1997.View Article |
| [35] | Balaghi, S., Mohammadifar, M.A., Zargaraan, A., “Physicochemical and rheological characterization of gum tragacanth exudates from six species of Iranian Astragalus,” Food Biophys, 5. 59-71.2010. |
| [36] | Phillips, G.O., Williams, P.A., Handbook of hydrocolloids, 2000, CRC Press. |
| [37] | Liu, J., Li, J.W., Tang, J., “Ultrasonically assisted extraction of total carbohydrates from Stevia rebaudiana Bertoni and identification of extracts,” Food Bioprod Process, 88. 215-221.2010. |
