1Shandong Lvyuan Agricultural Science and Technology Research Institute, Weifang, China
2Affiliated Hospital of Weifang Medical College, Weifang, China
3Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, China
4Institute of Agricultural Quality Standards and Testing Technology Research, Shandong academy of agricultural sciences, Jinan, China
The cruciferous herb Weixian radish (Raphanus sativus L.), also known as Lai Fu, is a popular type of edible vegetable. The present paper describes the molecular tracking and bioactivity of glucocerebroside isolated from Weixian radish for the first time. Methanol extraction was carried out by using silica gel, Sephadex LH-20 and RP-C18 column chromatography. Based on their structures, the two compounds were determined to be 1-O-(β-D-glucopyranosyl)-(2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxyl-tetracos-15'-enoyl amino]-8-octa-decene- 1, 3, 4-triol (glucocerebroside). The glucocerebroside could inhibit the growth of BEL-7402 cancer cells and induce apoptosis in these cells.
| [1] | Zhang, X.C., Modern and Practical Anti-cancer Medicine. Beijing Science and Technology Press: Beingjing, China, 1999, 185-187. |
| [2] | Li, S.Z., Graphic Compendium of Material Medical, Shanxi Normal University Press: Shan xi, China; 2007, 403. |
| [3] | Wei, G.H., Zhao, P.J., Wan, C.Y., Tian, Y. and Li, G.F., “Root vegetables fruits-radish of medicinal value, method of storage and preserving the family”, The First Academic Exchange Conference of the Medicated Diet and Health Proceeding, 187-188. 2009. |
| [4] | Curtis, I.S., “The noble radish: past, present and future”, Trends in Plant Science, 8, 305-307. Jul. 2003.View Article |
| [5] | I. Blazevic, J., Mastelic, “Glucosinolate degradation products and other bound and free volatiles in the leaves and roots of radish (Raphanus sativus L.)”, Food Chemistry, 113, 96-102. Nov. 2009.View Article |
| [6] | Rumiana, K., Martin, C., “Phases and phase transitions of the sphingolipids”, Biochimica et Biophysica Acta, 1255, 213-236. May. 1995. |
| [7] | Ryuichi, H.，Masahiko, K.，Tetsuya, K., “Structures of three new cerebrosides, astrocerebroside A, B, and C and of related nearly homogeneous cerebrosides”, Liebigs Annalen der Chemie, 7, 659-663. 1991. |
| [8] | Fattorusso, E., Mangoni, A., Progress in the chemistry of natural products. Springer Wien: New York, America, 1997, 72, 215-301. |
| [9] | Pei, D., Liu, J.X. and Di, D.L., “Cerebroside and ceramide from the pollen of Brassica napus L”, Fitoterapia, 81, 838-843. Oct. 2010.View Article |
| [10] | Rubinstein, I., Goad, L.J., Clagueb, A.D.H. and L. J. Mulheirn, “The 220 MHz NMR spectra of phytosterols”, Phytochemistry, 15, 195-200. Jan. 1976.View Article |
