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Preparation and Properties of Purified Phytase from Oakbug Milkcap (Lactarius Quietus) Immobilised on Coated Chitosan with Iron Nano Particles and Investigation of Its Usability in Food Industry

Hicran Onem, Hayrunnisa Nadaroglu

Journal of Food and Nutrition Research. 2014, 2(12), 938-945 doi:10.12691/jfnr-2-12-13
  • Figure 1. Purification of phytase by ion exchange chromatography using DEAE-sephadex
  • Figure 2. SDS-PAGE electrophoretic pattern of phytase [purified phytase enzyme from Oakbug Milkcap (Lactarius quietus)
  • Figure 3. The effect of time to the immobilization of phytase enzyme onto modifed chitosan with nano magnetite
  • Figure 4. SEM images I: nature chitosan; II: modified chitosan with nano magnetite; III: purified phytase immobilized modified chitosan with nano magnetite
  • Figure 5. XRD photographs for nature chitosan; modified chitosan with nano magnetite; purified phytase immobilized modified chitosan with nano magnetite
  • Figure 6. FT-IR spectrum of nature chitosan (a), modified chitosan with nano magnetite (b), immobilized chitosan with purified phytase from Oakbug milkcap (Lactarius quietus) (c)
  • Figure 7. Optimization of purified and immobilized phytase enzyme activity. Reaction condition for a reaction time optimization (temperature 60°C and pH 6.0), b pH optimization (temperature 60°C and incubation time 30 min), and c temperature optimization (pH 6.0 and incubation time 30 min)
  • Figure 8. (a) Thermal stability, (b) pH stability for free and immobilized phytase