The aim of this study was to evaluate the in vitro antioxidant, in-vivo immunomodulatory, and bioavailability profiles of Emblica officinalis (Storg C) in comparison to synthetic vitamin C using SD rats. The antioxidant activity of Storg C and synthetic vitamin C was assessed in vitro using the DPPH assay. Immunosuppression was induced by the administration of cyclophosphamide subcutaneously in all treated groups, and treatment with the test drug was continued for 14 days. For bioavailability analysis, a single oral dose of 200 mg/kg of Storg C and synthetic vitamin C was given to the rats, and pharmacokinetic parameters were measured at various time intervals using the LC-MS/MS method. Storg C exhibited significant free radical scavenging activity with an IC50 value of 44.24 µg/mL, outperforming the standard BHT (IC50 60.52 µg/mL) but slightly less effective than synthetic vitamin C (IC50 33.15 µg/mL). In immunomodulatory tests, Storg C at doses of 50 and 100 mg/kg enhanced both primary and secondary immune responses in a dose-dependent manner, showing better performance in blood indicators and immune response compared to the control, negative groups, and synthetic vitamin C. Additionally, Storg C demonstrated superior bioavailability, achieving a maximum plasma concentration of 135.68 ng/mL and synthetic vitamin C (126 ng/mL) at a peak time of 1 hour. These findings suggest that Storg C has notable antioxidant and immunomodulatory properties, along with enhanced bioavailability, making it a promising alternative to synthetic vitamin C.
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