Fruit wastes have attained considerable interest in the modern trading industry. Globally, almost 50% of the fruits agriculturally produced are processed in the beverage industry for juices, and almost 25 million tons of fruit wastes are being generated only from citrus fruits. In Pakistan, in the beverage industry, are being processed 34% of Citrus fruits, so a large volume of waste is being produced annually. The basic aim of the research work was to evaluate the potential bioactive components especially vitamin C and phenolic contents. The fruit wastes of five species of the citrus family selected for the research. Vitamin C and the Phenolic contents were estimated by High-performance liquid chromatography and Folin Ciocalteau respectively. The Grapefruit showed the highest contents of vitamin C i.e. 114.92 ± 0.36mg/100g and 110.56 ± 0.415mg/100g in pulp and peel respectively. Similarly the peel of oranges showed highest contents of phenolic compounds as compare to the peels of the other members of citrus family i.e. 155.1165 ± 0.1355mg/100g while the pulp of fruiter showed the highest concentration of phenolic compounds i.e. 119.9342 ± 0.194mg/100g as compare to the pulp of other citrus members. Due to the presence of a reasonable amount of Vitamin C and phenolic contents in fruit peel and pulp samples of citrus family, it can be concluded that the result of the present research would be fruitful for the treatment of deficiencies of these bioactive compounds.
| [1] | Syed, J.H., et al., Pesticide residues in fruits and vegetables from Pakistan: a review of the occurrence and associated human health risks. Environmental Science and Pollution Research, 2014. 21(23): p. 13367-13393.View Article PubMed |
| [2] | Ali, S.W., M.A. Mateen Ahmad, and A. Ahmed, Management of Citrus Waste in Pakistan’s Perspective. Journal of Hygienic Engineering and Design, 2016. 15: p. 83-88. |
| [3] | James, O. and U.C. Emmanuel, Comparative studies on the protein and mineral composition of some selected Nigerian vegetables. African Journal of Food Science, 2011. 5(1): p. 22-25. |
| [4] | Chen, Y., et al., Evaluation of phenolic composition and antioxidant properties of different varieties of Chinese citrus. Food Chemistry, 2021. 364: p. 130413.View Article PubMed |
| [5] | Ishfaq, M., et al., Antioxidant and wound healing potential of essential oil from Citrus reticulata peel and its chemical characterization. Current Pharmaceutical Biotechnology, 2021. 22(8): p. 1114-1121.View Article PubMed |
| [6] | Rojas-Lema, S., et al., On the use of phenolic compounds present in citrus fruits and grapes as natural antioxidants for thermo-compressed bio-based high-density polyethylene films. Antioxidants, 2021. 10(1): p. 14.View Article PubMed |
| [7] | Asencio, A.D., et al., Organic acids, sugars, antioxidant activity, sensorial and other fruit characteristics of nine traditional Spanish Citrus fruits. European Food Research and Technology, 2018. 244(8): p. 1497-1508.View Article |
| [8] | Chambial, S., et al., Vitamin C in disease prevention and cure: an overview. Indian journal of clinical biochemistry, 2013. 28(4): p. 314-328.View Article PubMed |
| [9] | Shah, D. and H. Sachdev, Vitamin C (Ascorbic acid) In: Behrman R., Kliegman R., Stanton, editors. Nelson Textbook of Pediatrics. 2012, Elsevier Science Philadelphia:.View Article PubMed |
| [10] | Agarwal, A., et al., Scurvy in pediatric age group–a disease often forgotten? Journal of clinical orthopaedics and trauma, 2015. 6(2): p. 101-107.View Article PubMed |
| [11] | Lovasz, G., et al., Skeletal manifestations of infantile scurvy in a late medieval anthropological series from Hungary. Anthropological Science, 2013: p. 130905.View Article |
| [12] | Baron, J.H., Sailors' scurvy before and after James Lind–a reassessment. Nutrition reviews, 2009. 67(6): p. 315-332.View Article PubMed |
| [13] | Smith, A., G. Di Primio, and S. Humphrey-Murto, Scurvy in the developed world. Cmaj, 2011. 183(11): p. E752-E755.View Article PubMed |
| [14] | Zacarías-García, J., et al., Antioxidant capacity in fruit of Citrus cultivars with marked differences in pulp coloration: Contribution of carotenoids and vitamin C. Food Science and Technology International, 2021. 27(3): p. 210-222.View Article PubMed |
| [15] | Wang, Y., et al., Citrus flavonoids and their antioxidant evaluation. Critical Reviews in Food Science and Nutrition, 2020: p. 1-22. |
| [16] | Zhang, H., Y.-f. YANG, and Z.-q. ZHOU, Phenolic and flavonoid contents of mandarin (Citrus reticulata Blanco) fruit tissues and their antioxidant capacity as evaluated by DPPH and ABTS methods. Journal of Integrative Agriculture, 2018. 17(1): p. 256-263.View Article |
| [17] | Sun, Y., et al., Flavonoids, phenolic acids, carotenoids and antioxidant activity of fresh eating citrus fruits, using the coupled in vitro digestion and human intestinal HepG2 cells model. Food chemistry, 2019. 279: p. 321-327.View Article PubMed |
| [18] | Singh, B., et al., Phenolic composition, antioxidant potential and health benefits of citrus peel. Food Research International, 2020. 132: p. 109114.View Article PubMed |
| [19] | Chen, Q., et al., Profiling of flavonoid and antioxidant activity of fruit tissues from 27 Chinese local citrus cultivars. Plants, 2020. 9(2): p. 196.View Article PubMed |
| [20] | İnan, Ö., M.M. Özcan, and F. Aljuhaimi, Effect of location and Citrus species on total phenolic, antioxidant, and radical scavenging activities of some Citrus seed and oils. Journal of Food Processing and Preservation, 2018. 42(3): p. e13555.View Article |
| [21] | Upadhyaya, S., E. Khatiwora, and D. Bora, Estimation of total Phenol and flavonoid contents of Citrus limon L Burmf leaves from North Eastern region of India. Journal of Drug Delivery and Therapeutics, 2019. 9(2-s): p. 40-42.View Article |
| [22] | Buyukkurt, O.K., et al., Characterization of phenolic compounds in sweet lime (Citrus limetta) peel and freshly squeezed juices by LC-DAD-ESI-MS/MS and their antioxidant activity. Journal of Food Measurement and Characterization, 2019. 13(4): p. 3242-3249.View Article |
| [23] | Wanna, C., Free radical scavenging capacity and total phenolic contents in peel and fleshy crude extracts of selected vegetables. Pharmacognosy Journal, 2019. 11(6).View Article |
| [24] | Mehmood, T., et al., Variation in phenolic acids and antibacterial attributes of peel extracts from ripe and unripe [Citrus limon (L.) Osbeck] fruit. Journal of Food Measurement and Characterization, 2020. 14(3): p. 1325-1332.View Article |
| [25] | Anahita, A., R. Asmah, and O. Fauziah, Evaluation of total phenolic content, total antioxidant activity, and antioxidant vitamin composition of pomegranate seed and juice. 2015.View Article |
| [26] | Sir Elkhatim, K.A., R.A. Elagib, and A.B. Hassan, Content of phenolic compounds and vitamin C and antioxidant activity in wasted parts of Sudanese citrus fruits. Food science & nutrition, 2018. 6(5): p. 1214-1219.View Article PubMed |
| [27] | Najwa, F.R. and A. Azrina, Comparison of vitamin C content in citrus fruits by titration and high performance liquid chromatography (HPLC) methods. International Food Research Journal, 2017. 24(2): p. 726. |
| [28] | Thieme, C., et al., Polyphenols, vitamin c, in vitro antioxidant capacity, α-amylase and COX-2 inhibitory activities of citrus samples from Aceh, Indonesia. International Journal for Vitamin and Nutrition Research, 2019. |
| [29] | Dalar, A., et al., Phenolic composition, antioxidant and enzyme inhibitory activities of Eryngium bornmuelleri leaf. Plant Foods for Human Nutrition, 2014. 69(1): p. 30-36.View Article PubMed |
| [30] | Shrestha, N., S. Shrestha, and A. Bhattarai, Determination of ascorbic acid in different citrus fruits of Kathmandu Valley. Journal of Medical and Biological Science Research, 2016. 2(1): p. 9-14. |
| [31] | Tareen, H., et al. Determination of Vitamin C content in Citrus Fruits and in Non-Citrus Fruits by Titrimetric method, with special reference to their nutritional importance in Human diet. in Biological Forum. 2015. Research Trend. |
| [32] | Ezekiel, A., et al., Evaluation of Ascorbic Acid Contents in Selected Fruits using Iodometric method and UV Spectrophotometer. Advances in Natural and Applied Sciences, 2018. 12(5): p. 21-24. |
| [33] | Diab, D.A. and N.A. Asaad, Comparative analysis of ascorbic acid content and antioxidant activity of some fruit juices in Syria. Research Journal of Pharmacy and Technology, 2018. 11(2): p. 515-520.View Article |
| [34] | Abd Rahman, N.F., et al., Effects of drying methods on total phenolic contents and antioxidant capacity of the pomelo (Citrus grandis (L.) Osbeck) peels. Innovative Food Science & Emerging Technologies, 2018. 50: p. 217-225.View Article |