Abstract

Three-harvest in two years is a traditional farming system in the Jinzhong Basin of Shanxi Province of the eastern Loess Plateau in China. Recently, an innovative farming system of winter wheat-fresh maize with two-harvest per year, making full use of temperature and light resources, is proposed. However, there is little known of the optimal nitrogen application rate for the innovative farming system to increase crop yield and improve ecological environment. In current study, six nitrogen application rates were carried out, consisting of winter wheat/fresh maize season 0/0 kg·ha^-1 (N₀, Control), 75/75 kg·ha^-1 (N₇₅), 150/150 kg·ha^-1 (N₁₅₀), 225/225 kg·ha^-1 (N₂₂₅), 300/300 kg·ha^-1 (N₃₀₀) and 375/375 kg·ha^-1 (N₃₇₅). The grain yield, dry matter and nitrogen accumulation and remobilization were analyzed to determine the optimal nitrogen application rate in the winter wheat-fresh maize cropping system in the Jinzhong Basin. The results showed that in the winter wheat and fresh maize season, compared with the N₀, N₇₅ and N₃₇₅ treatments, total dry matter accumulation at winter wheat maturity and fresh maize harvesting increased significantly by 82.9%-94.5% and 26.1%-197.1%, respectively, and dry matter remobilization in leaves and stem+sheath (+tassel) increased significantly under the N₁₅₀ treatment. Total nitrogen accumulations under the N₁₅₀, N₂₂₅ and N₃₀₀ treatments increased significantly by 58.2%-162.9% and 55.7%-301.7% compared with those under the N₀ and N₇₅ treatments, respectively. Nitrogen remobilization in the kernel and stem+sheath (+tassel) under the N₁₅₀ treatment increased significantly compared with the N₀ and N₇₅ treatments. In addition, nitrogen partial factor productivity of winter wheat and fresh maize under the N₇₅ and N₁₅₀ treatments increased significantly by 36.7%-534.1% and 45.2%-390.4% compared with the other nitrogen treatments, respectively, and nitrogen uptake efficiency also increased significantly by 79.1%-321.4% and 58.3%-239.5%, respectively. The yield of winter wheat and fresh maize under the N₂₂₅ treatment was the highest, which significantly increased by 54.1% and 71.6% compared with that under the N₀ treatment, respectively. In summary, nitrogen application rates of 150-225/150-225 kg·ha^-1can be potential recommended use amounts with higher crop yield and nitrogen use efficiency in winter wheat-fresh maize cropping system in the Jinzhong Basin.

1. Introduction

Nitrogen is one of the essential nutrient elements for crop growth, and which can directly affect photosynthesis, dry matter accumulation, crop growth and development ¹. To meet the food demand in China, large amounts of nitrogen fertilizer have been applied in agricultural production for many decades. However, most of the nitrogen that cannot be absorbed by crops loss to the surrounding environment in various forms of reactive nitrogen due to low nitrogen use efficiency ². Soil nitrogen loss caused a series of serious environmental problems such as greenhouse effects, water eutrophication, acid rain, groundwater nitrate pollution and ozone layer destruction ^{3, 4}. At the same time, excessive nitrogen application also leads to the reduction of crop yield and quality, as well as, other problems ^{5, 6, 7}. Rational nitrogen application can not only relieve on the environment pressure, but also improve crop yield and quality ^{8, 9, 10, 11, 12}.

Numerous studies have shown that rational nitrogen application can promote crop dry matter accumulation ^{13, 14, 15}, improve nitrogen translocation and remobilization ^{16, 17, 18}, and increase crop yield ^{19, 20, 21}. An et al. ²² reported that the nitrogen accumulation in roots, stems, leaves and spikes of winter wheat showed an increasing trend with increasing nitrogen application rates (0-300 kg·ha^-1) in Henan Province, while the recovery rate of nitrogen fertilizer showed a significantly decreasing trend. Nitrogen partial factor productivity of winter wheat and summer corn decreased gradually with increasing nitrogen application rate under the drip irrigation winter wheat-summer corn cropping system in the Jiaozhou region of Shandong Province, adoption of 210 kg·ha^-1 in the winter wheat season and 225 kg·ha^-1 in the summer maize season improved annual crop yield and nitrogen use efficiency ²³. Belete et al. ²⁴ found that with the increase in nitrogen application (0-360 kg·ha^-1), the yield, grain nitrogen uptake, plant nitrogen uptake of bread wheat increased in Ethiopia, however, nitrogen agronomic efficiency, agro-physiological efficiency and apparent recovery efficiency showed a significantly decreasing trend. Due to differences in climatic conditions, soil types, cropping systems and crop varieties, the optimal nitrogen application rate, nitrogen accumulation and remobilization, nitrogen use efficiency and crop yield are different in different studies.

Three-harvest in two years is a traditional farming system in the Jinzhong Basin of Shanxi Province of the eastern Loess Plateau in China. Due to insufficient accumulated temperature and heat, it is generally difficult for common grain maize to mature after winter wheat harvest in current region. In recent years, fresh maize is becoming more popular for the public due to its sticky, fragrant and tender taste and abundant bioactive substances such as multiple vitamins, amino acids and minerals. Therefore, the planting area of fresh maize is increasing recently ²⁵. Now, the planting area of fresh maize in China exceeds 1.34 million hectares (ha). and China is the largest producer and consumer of fresh maize in the world ²⁶. Because economic benefit is lager for fresh maize than common grain maize, the planting area of fresh maize increase continuously in the Jinzhong Basin ²⁷. At the same time, planting fresh maize after winter wheat harvest in the Jinzhong Basin become a possibility, because due to shorter growth period of fresh maize and the increasing accumulated temperature under climate change. Therefore, an innovative farming system of winter wheat-fresh maize with two-harvest per year is gradually developing, which is favor of improve food production and further planting benefit. At present, there is little known about the effect of nitrogen application rate of crop yield, dry matter, nitrogen accumulation and remobilization under the innovative farming system in the Jinzhong Basin. Therefore, the objectives of this study were to (i) analyze the effects of nitrogen application rate on dry matter accumulation and remobilization, (ii) evaluate nitrogen accumulation and remobilization, nitrogen use efficiency, (iii) identity crops yield and its composition, (iv) to determine the optimal nitrogen application rate for the innovative farming system of winter wheat-fresh maize with two-harvest per year in the Jinzhong Basin of Shanxi Province of the eastern Loess Plateau in China.

2. Materials and Methods

The experiment was conducted at the Agricultural Valley Experimental Station (37° 25′ N, 112° 33′ E) of Shanxi Agricultural University, Mengjiazhuang Village, Taigu District, Jinzhong City, Shanxi Province (Figure 1). The region has a monsoon climate at medium latitudes, with a mean annual temperature of 10.6°C, a mean annual precipitation of 450-490 mm, and a frost-free season of 160-165 days. The basic soil properties in the 0-20 cm layer was 28.89 g·kg^-1 of soil organic matter, 42.26 mg·kg^-1 of alkali-hydrolysable nitrogen, 15.04 mg·kg^-1 of available phosphorus, 205.69 mg·kg^-1 of available potassium, and pH 7.80. During the experiment, the precipitation was 363.6 mm and the average daily temperature was 11.5°C in the region (Figure 2).

The experiment was carried out with a random block design. Six nitrogen application rates were set up, including 0/0 kg·ha^-1 (N₀), 75/75 kg·ha^-1 (N₇₅), 150/150 kg·ha^-1 (N₁₅₀), 225/225 kg·ha^-1 (N₂₂₅), 300/300 kg·ha^-1 (N₃₀₀) and 375/375 kg·ha^-1 (N₃₇₅) for winter wheat/fresh maize season, The N₀ treatment was used as the control. Each treatment was replicated three times, and the plot size was 2 m×6 m (12 m²). Some panels with polyvinyl chloride, with the burying depth of approximately 20 cm underground and extending approximately 20 cm above ground, were used to isolate adjacent plots to prevent nitrogen and water runoff. Urea was applied to set up different nitrogen treatments in the study. All plots received 150 kg·ha^-1 phosphorus fertilizer (P₂O₅) and 90 kg·ha^-1 potassium fertilizer (K₂O) before winter wheat and fresh maize were sown.

The variety for winter wheat was Jintai 182, which was sown with a seeding amount of 300 kg·ha^-1 on October 3, 2020. Nitrogen fertilizers were used as the basal dose before sowing and topdressing at the jointing stage with a ratio of 1:1. All plots were irrigated with the volume of 160 m³·ha^-1 on November 26, 2020, 90 m³·ha^-1 on April 10, 2021 and 89.2 m³·ha^-1 on April 24, 2021, respectively. Winter wheat was harvested for all plots on June 18, 2021.

The variety of fresh maize was Jinnuo 20. After the wheat straw was returned to the field. Urea was used as basal dose. Fresh maize was sown at a density of 52,500 plants·ha^-1 with 30 cm of narrow rows and 70 cm of wide rows on June 20, 2021. All plots were irrigated with the volume of 1916.7 m³·ha^-1 on August 14, 2021. Fresh maize was harvested for all plots on September 15, 2021.

In this study, uniform and representative plants (12 to 30 plants of winter wheat, 3 plants of fresh maize) were sampled and analyzed at the overwintering, anthesis and maturity stages for winter wheat and at the jointing, silking and harvesting stages for fresh maize. Different plant organs were divided at the anthesis and maturity stages for winter wheat, and at the silking and harvesting stages for fresh maize. Then, the dry matter of each organ was weighed. Thereinto, those organs for winter wheat comprised of leaves, stem + sheath and cob + glume at the anthesis stage, and leaves, stem + sheath, cob + glume and kernel at the maturity stage. In addition, those organs for fresh maize comprised of leaves, stem + sheath + tassel, and cob + husk at the silking stage, and leaves, stem + sheath + tassel, cob + husk and kernel at the harvest. The plants or separated organs were put into a kraft paper bag, after dried at 105°C for 30 min, dried to constant weight at 75°C and weighed. Dry matter remobilization of each organ was calculated according to equation (1). The sum of dry matter remobilization of different organs was dry matter accumulation.

(1)

where DMR is the dry matter remobilization of each organ at the winter wheat maturity stage/fresh maize harvesting (kg·ha^-1), DMW is the dry matter weight of each organ at the winter wheat maturity stage/fresh maize harvesting (kg·plant^-1), and DP is the population quantity of winter wheat at the maturity stage/fresh maize at the harvest (plant·ha^-1).

The Tissuelyser-II High Throughput Tissue Grinder (QIAGEN, Germany) was used to crush all sample of plant organs. The nitrogen content of plant sample was determined by the Kjeldahl method using the Kjeldahl nitrogen analyzer (OLB9870) ²⁸. According to equation (2), the nitrogen remobilization of each organ was calculated. The sum of the nitrogen remobilization of different organs was the nitrogen accumulation. In addition, nitrogen partial factor productivity (NPFP), nitrogen uptake efficiency (NUpE), nitrogen use efficiency (NUE), and nitrogen harvest index (NHI) were calculated according to equation (3-6) ^{30, 31}.

(2)

(3)

(4)

(5)

(6)

where, NR is the nitrogen remobilization of each organ at the winter wheat maturity stage/fresh maize harvesting stage (kg·ha^-1), NC is the nitrogen content of each organ at the winter wheat maturity stage/fresh maize harvesting stage (%), and DMR is the same as equation (1). NPFP is nitrogen partial factor productivity (kg·kg^-1), Y is crop yield (kg·ha^-1), Nr is nitrogen application rate (kg·ha^-1), NUpE is nitrogen uptake efficiency (%), NA_f is plant nitrogen accumulation at winter wheat maturity stage/fresh maize harvesting stage in nitrogen application area (kg·ha^-1), NUE is nitrogen use efficiency (%), NA_c is nitrogen accumulation of all plant at winter wheat maturity stage/fresh maize harvesting stage under N₀ treatment (kg·ha^-1), NHI is nitrogen harvest index (%), NK is the nitrogen remobilization amount of grain kernel at winter wheat maturity stage/fresh maize harvesting stage (kg·ha^-1), NA is the nitrogen accumulation amount of all plant at winter wheat maturity stage/fresh maize harvesting stage (kg·ha^-1).

At the maturity stage, winter wheat with uniform growth and unsampled was harvested in an area of 0.667 m². Meanwhile, total spike numbers of winter wheat were recorded. Twenty spikes were taken to determine the kernel number per spike. Then, 1000 kernels were weighed, which was replicated three times. In addition, the grain kernel weight of all sampled spikes per plot was reported to obtain the actual yield after converting to that with 14% of grain water content. At the harvesting stage for fresh maize, all ears were harvested in each plot. The effective ear number was counted and weighed, which was the actual yield. Ten uniform ears of fresh maize were selected to determine the number of kernels per ear and 1000-kernel weight ³¹.

Analysis of variance was used to test for treatment effect by SPSS 17.0 software. The mean differences between different treatments were separated by the new multiple range test (Duncan) at p<0.05. The map was drawn using ArcGIS 10.7, and other figures were plotted using Origin 2021.

3. Results

Dry matter accumulations of winter wheat and fresh maize at key growth stages under different treatments were analyzed in Figure 3. In the winter wheat growth season, dry matter accumulation under all nitrogen application treatments at the overwintering stage increased significantly by 114.7%-227.1% compared with that under the N₀ treatment. Dry matter accumulation under the N₃₇₅ treatment was the highest, and which increased significantly by 35.5%-52.4% compared with those udner the N₇₅, N₁₅₀ and N₃₀₀ treatments. Dry matter accumulations under the N₁₅₀ treatment were the highest at both the anthesis and maturity stages, and which increased significantly by 41.6%-94.5% compared with those under the N₀, N₇₅ and N₃₇₅ treatments (Figure 3 I). The N₂₂₅ and N₃₀₀ treatments were significantly larger dry matter accumulation by 37.2%-62.6% compared with N₀, N₇₅ and N₃₇₅ treatments at the maturity stage. In the growth season of fresh maize (Figure 3 II), dry matter accumulation under the N₁₅₀ treatment increased significantly by 37.8%-112.0% compared with those under the other treatments. At the silking stage, dry matter accumulation of fresh maize under N₁₅₀, N₂₂₅, N₃₀₀ and N₃₇₅ treatments increased significantly by 45.0%-72.6% compared with that under the N₀ treatment, and which under the N₃₀₀ treatment was significantly higher 38.2% compared that with N₇₅ treatment. At the harvesting stage, dry matter accumulations of fresh maize under all nitrogen application treatments increased significantly by 123.7%-197.1% compared with that under the N₀ treatment. The N₁₅₀ treatment was the highest dry matter accumulation, which was significantly lager 32.8% and 26.1% compared with the N₇₅ and N₃₇₅ treatments, respectively.

At the maturity stage of winter wheat (Figure 4 I), dry matter remobilization in the kernel, leaf and stem + sheath under the N₁₅₀ treatment significantly increased by 32.7%-104.8%, 44.2%-169.9% and 17.6%-93.5% compared with those under the other treatments, respectively. Compared with the N₀, N₇₅ and N₃₇₅ treatments, the N₂₂₅ and N₃₀₀ treatments increased significantly dry matter remobilizations in the kernels and stems + sheaths by 36.7%-54.3% and 23.2%-64.5%, respectively. When fresh maize was harvested (Figure 4 II), dry matter remobilization in kernel under all nitrogen application treatments increased significantly by 121.9%-234.0% compared with that under the N₀ treatment. Dry matter remobilization in the cob + husk under the N₁₅₀ treatment was significantly higher 222.1% and 34.8% compared with those under the N₀ and N₇₅ treatments, which in the leaf significantly increased by 140.0%, 17.7% and 17.9% compared with those under the N₀, N₇₅ and N₃₇₅ treatments, respectively. Dry matter remobilization in stem + sheath + tassel under the N₁₅₀ treatment increased significantly by 17.1%~195.3% compared with other treatments.

At the maturity stage of winter wheat (Figure 5 I), except for the N₃₀₀ treatment, nitrogen remobilization in kernel followed the order of N₁₅₀ > N₂₂₅ > N₃₇₅ > N₇₅ > N₀ (p<0.05), and the nitrogen application treatment significantly increased by 48.4%-224.5% compared with the N₀ treatment. The nitrogen remobilization in cob + glume under the N₃₀₀ treatment increased significantly by 85.1% and 153.1% compared with that under the N₇₅ and N₃₇₅ treatments, and which under the N₁₅₀ and N₂₂₅ treatments increased significantly by 129.1% and 97.1% compared with that under the N₃₇₅ treatment. Nitrogen remobilization in leaf under the N₁₅₀ treatment increased significantly by 61.8%-206.6% compared with those under the others treatments. Nitrogen remobilization in the stem + sheath under the N₁₅₀, N₂₂₅ and N₃₀₀ treatments significantly increased by 41.5%-91.7% compared with those under the other treatments. When fresh maize was harvested (Figure 5 II), nitrogen remobilizations in kernel under nitrogen application treatments increased significantly by 130.0%-286.7% compared with that under the N₀ treatment, and by 67.7% and 61.9% for N₁₅₀ and N₂₂₅, respectively, compared with N₇₅. Nitrogen remobilization in the cob + husk under the N₁₅₀, N₂₂₅ and N₃₀₀ treatments increased significantly by 35.1%-261.7% compared with those under the N₀ and N₇₅ treatments, which under the N₃₇₅ treatment was significantly higher by 210.3% compared with that under the N₀ treatment. Nitrogen remobilizations in leaf under the N₁₅₀, N₂₂₅, N₃₀₀ and N₃₇₅ treatments were higher by 281.7% and 55.1%-73.6% than those under the N₀ and N₇₅ treatments, respectively, and there was also a significant difference between the latter two treatments. Nitrogen remobilizations in stem + sheathing + tassel under the N₁₅₀, N₃₀₀ and N₃₇₅ treatments increased significantly by 15.8%-405.7% compared with those under the N₀, N₇₅ and N₂₂₅ treatments, following the order of N₂₂₅>N₇₅>N₀ (p<0.05).

With the increase in nitrogen application rate, the NPFP of winter wheat showed a gradually decreasing trend (Table 1) as N₇₅>N₁₅₀>N₂₂₅>N₃₇₅ (p<0.05), while NUpE and NUE showed a trend of first increasing and then decreasing. The highest NUpE and NUE under the N₁₅₀ treatment were observed among all treatments. The N₁₅₀ treatment increased significantly by 92.9%~321.4% NUpE compared with the N₂₂₅, N₃₀₀ and N₃₇₅ treatments, and by 133.9%-627.8% NUE compared with other nitrogen application treatments. Compared with that under the N₀ treatment, the NHI under all nitrogen application treatments increased significantly by 16.5%-27.6%. The NHI under the N₃₇₅ treatment increased significantly by 9.5% compared with that under the N₃₀₀ treatment. Both the NPFP and NUpE for fresh maize were the order of N₇₅>N₁₅₀>N₂₂₅>N₃₀₀>N₃₇₅ (p<0.05), except that there was no significant difference in NPFP between N₃₀₀ and N₃₇₅ treatments. With the increase in nitrogen application rate, NUE showed a trend of first increasing and then decreasing. The NUE under the N₇₅ and N₁₅₀ treatments were greater significantly by 54.4%-190.1% compared with those in the other treatments.

The actual yield of winter wheat under the N₇₅, N₁₅₀, N₂₂₅ and N₃₀₀ treatments increased by 25.7%-54.1% compared with those of the N₀ and N₃₇₅ treatments (Table 2). The theoretical yield of winter wheat under the N₂₂₅ treatment increased by 34.4%-44.3% compared with those under the N₀, N₁₅₀ and N₃₀₀ treatments. Spike number, kernel number per spike, the theoretical yield and the actual yield of fresh maize under all nitrogen application treatments significantly increased by 15.4%-25.0%, 34.8%-50.4%, 35.5%-70.9% and 39.9%-71.6% compared with those under the N₀ treatment, respectively. However, the 1000-kernel weight of fresh maize under all nitrogen application treatments were lower significantly by 9.1%-13.3% compared with these under the N₀ treatment (except the N₂₂₅ treatment). In addition, kernel number per spike under the N₃₀₀ treatment increased significantly by 8.2% and 11.6% compared with these under the N₁₅₀ and N₃₇₅ treatments. The 1000-kernel weight under the N₂₂₅ treatment increased significantly by 9.6% compared with that under the N₃₇₅ treatment.

4. Discussion

In the current study, dry matter accumulation, nitrogen accumulation and remobilization in grain kernel for winter wheat and fresh maize at harvest showed the tread of first increasing and then decreasing with the increase in nitrogen application rate, which was the highest under the N₁₅₀ treatment. In addition, nitrogen application significantly increased dry matter accumulation, nitrogen accumulation and remobilization in grain kernel than the N₀ treatment, except for the accumulation of dry matter in winter wheat. This is because nitrogen application promotes the growth and development of crops, increases the leaf area and root biomass, promotes dry matter and nitrogen accumulation and remobilization ³². Similar changes in dry matter accumulation, nitrogen accumulation and remobilization with the increase in nitrogen application rate were observed in winter wheat and summer corn cropping system in Jiaozhou, Shandong Province, however, which were the highest at nitrogen application rate of 270 kg·ha^-1 for winter wheat and 300 kg·ha^-1 for summer maize ²³. The optimal nitrogen application rate was higher than that in our study. This may be due to the difference in climatic conditions, soil characteristics, crop varieties, other farmland management measures.

The yield of winter wheat and fresh maize under the N₂₂₅ treatment in this study was the highest compared with other treatments, which was consistent with those previous results ^{33, 34}. This may mainly be related to the increase in spike number and kernel number per spike. For winter wheat, insufficient nitrogen application rate could lead to a decrease in plant population and spike number, thus limiting the increase in the yield ³⁵. Although excessive nitrogen application rate could increase plant population of winter wheat, larger plant population would limit the development of wheat spikes. This could result in the decrease in kernel number and kernel weight per spike, ultimately leading to the yield reduction ⁵. For fresh maize, nitrogen application can promote spikelet differentiation and prevent kernel abortion ³⁶, therefore, spike number and kernel number per spike under all nitrogen application treatments were significantly higher than those under the N₀ treatment. In addition, reasonable nitrogen application can prolong the filling duration of kernel, and increase the 1000-kernel weight and the yield ³⁸. However, the 1000-kernel weights of fresh maize under all nitrogen application treatments were lower than that under the N₀ treatment in this study. It may be because the growth stage of fresh maize under the N₀ treatment was shortened, which lead to higher kernel maturity and relatively more dry matter accumulation ³⁸. However, the growth processes of fresh maize under nitrogen application treatments were prolonged with lower kernel maturity and relatively less dry matter accumulation, resulting in a lower 1000-kernel weight.

Generally, the NPFP, NUpE and NUE of winter wheat gradually decreased with the increase in nitrogen application rate ^{39, 40}, which was basically consistent with the results of the study. In the study, the NPFP, NUpE and NUE of winter wheat and fresh maize under the N₇₅ and N₁₅₀ treatments were higher than those under others nitrogen application treatments. However, the NUEs under most of treatments were generally lower than 40% and even than 25% in the study, which were lower than those of mean NUE for three major food crops in China ⁴¹. Lower NUE may be related to the fertilization method and fertilization depth ⁴². In the study, nitrogen fertilizer in the winter wheat season was applied as the basal dose before sowing and topdressing at the jointing stage with a ratio of 1:1, and artificial application was adopted. Although rotary tillage was carried out after fertilization, the depth was shallower and more wasteful than strip application. In addition, urea was applied only within narrow rows, and rotated to the depth of approximately 5 cm by micro-rotovator before fresh maize was sown. In addition, no fertilizer was applied within wide rows in the fresh maize season. This may lead to low nitrogen uptake and use of fresh maize. Lower crop yield in the current study may also be responsible for lower NUE. In the study, there were less precipitation and insufficient irrigation during winter wheat and fresh maize growth seasons, which resulted in less nitrogen uptake and lower NUE. In addition, lower NUE of fresh maize may be related to its incomplete maturity. At the harvesting, the plant of fresh maize absorbs and translocates less nitrogen to the kernel under incomplete maturity than full maturity.

In this study, it was difficult to meet the water demand of crops due to the low precipitation during the experiment. Although low irrigation was implemented during the growth for winter wheat and fresh maize, inadequate irrigation resulted finally in lower crop yields and nitrogen use. Water deficit can affect nitrogen uptake and use. In future research, we will assess the effect of nitrogen application rate on dry matter and nitrogen accumulation and remobilization under the condition of sufficient water irrigation, to provide a reliable basis for rational nitrogen application rate in winter wheat-fresh maize cropping systems in the Jinzhong Basin. In addition, the experiment was only carried out for one year, and the implement duration was shorter. The representativeness of the results could be limited at a certain extent. It is necessary to prolong the experimental duration in the further research.

5. Conclusions

Compared with the N₀ and N₇₅ treatments, adoption of the N₁₅₀ and N₂₂₅ treatments increased dry matter and nitrogen accumulation, dry matter remobilization in the cob + glume (husk) and stem + sheath (tassel) organs, and nitrogen remobilization in the kernel and stem + sheath (tassel) organs for winter wheat at the maturity stage and fresh maize at the harvesting. There was NPFP, NUpE, NUE, NHI, the yield and its components for winter wheat and fresh maize under the N₁₅₀ and N₂₂₅ treatments. In conclusion, 150-225 kg·ha^-1 for winter wheat and 150-225 kg·ha^-1 of fresh maize will be potential optimal nitrogen application rates for the innovative farming system of winter wheat-fresh maize with two-harvest per year in the Jinzhong Basin of Shanxi Province of.the eastern Loess Plateau in China.

Acknowledgements

This research was supported by Special project of Biological Breeding for Shanxi Agricultural University (YZGC089).

References