Figure 7. The protein levels of NF-κB (p65) and Iκ-Bα in HaCaT whole cells. The cells were pretreated with 0, 0.1% DMSO, 10, 20, 40, and 80 μg/mL of DCM fraction for 2 h and then stimulated with IFN-γ and TNF-α for 18 h. After lysis the cell, the level of NF-κB (p65) and Iκ-Bα in whole cells were examined by western blotting. The intensity of band (A) was quantified and presented as the bar graph (B). GAPDH were used as internal controls. V, vehicle (serum free medium), C, control (DMSO+IFN-γ and TNF-α).

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Orostachys japonicus Suppresses IFN-γ and TNF-α Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-κB

Yoo Jung Jeon, Sung Hyun Kim, Dong Seok Lee

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