Figure 3. Eucommia ulmoides (EU) inhibits osteoarthritis (OA) pathogenesis. (A, B) qRT-PCR analysis of rat cartilage tissues. The mRNA levels of Mmp3, Mmp13, Col2a1 (A), and Fpr2 (B) were determined by real-time PCR, and the values were normalized to β-actin expression. (C-E) Immunohistochemical analyses of MMP13 (C), COL2A1 (D), and FPR2 (E) in OA articular cartilage from monoiodoacetate (MIA)-injected rats compared with those in sham-operated rats (n = 4). Scale bars = 100 m. Values are expressed as the mean ± SEM. One-way ANOVA was performed followed by Dunnett’s multiple comparisons test. MMP3, Matrix Metallopeptidase 3; MMP13, Matrix Metallopeptidase 13; COL2A1, Collagen Type II Alpha 1 Chain; FPR2, Formyl Peptide Receptor 2. Pound keys (#) indicate statistical significance (#P < 0.05, ##P < 0.05, ###P < 0.005) relative to Sham operated group. Asterisks (*) indicate statistical significance (*P < 0.05, **P < 0.05, ***P < 0.005) comparing EU-treated groups relative to MIA injected group : Eucommia ulmoides Prevents Articular Cartilage Destruction via FPR2-LXA4 in the Rat Monoiodoacetate Model of Osteoarthritis : Science and Education Publishing

Figure 3. Eucommia ulmoides (EU) inhibits osteoarthritis (OA) pathogenesis. (A, B) qRT-PCR analysis of rat cartilage tissues. The mRNA levels of Mmp3, Mmp13, Col2a1 (A), and Fpr2 (B) were determined by real-time PCR, and the values were normalized to β-actin expression. (C-E) Immunohistochemical analyses of MMP13 (C), COL2A1 (D), and FPR2 (E) in OA articular cartilage from monoiodoacetate (MIA)-injected rats compared with those in sham-operated rats (n = 4). Scale bars = 100 μm. Values are expressed as the mean ± SEM. One-way ANOVA was performed followed by Dunnett’s multiple comparisons test. MMP3, Matrix Metallopeptidase 3; MMP13, Matrix Metallopeptidase 13; COL2A1, Collagen Type II Alpha 1 Chain; FPR2, Formyl Peptide Receptor 2. Pound keys (#) indicate statistical significance (#P < 0.05, ##P < 0.05, ###P < 0.005) relative to Sham operated group. Asterisks () indicate statistical significance (P < 0.05, P < 0.05, *P < 0.005) comparing EU-treated groups relative to MIA injected group

Journal of Food and Nutrition Research. 2022, 10(4), 313-320 doi:10.12691/jfnr-10-4-8