Figure 2. Pre- and post-exposure of D. magna to CAE increases survival to lead toxicity. Ten 3-day old Daphnia neonates were used in the acute toxicity assays for each set-up, and the number of surviving neonates were recorded every hour for a 24-hour period. All the survival plots from Figure 1 are superimposed (with non-solid colored markers) in panels A to C for comparison with the assorted pre- and post-exposure assay designs in A) 10 ug/mL, B) 15 ug/mL, and C) 20 ug/mL CAE with survival plots normalized to no extract/ no Pb (control). D) Recorded hour of first and last deaths, and hour death reached 50% within the 24-hour observation period (nr = did not reach 50% death) : Crude Anthocyanin Extract (CAE) from Ballatinao Black Rice Reduces Acute Lead Toxicity in Daphnia magna : Science and Education Publishing

Figure 2. Pre- and post-exposure of D. magna to CAE increases survival to lead toxicity. Ten 3-day old Daphnia neonates were used in the acute toxicity assays for each set-up, and the number of surviving neonates were recorded every hour for a 24-hour period. All the survival plots from Figure 1 are superimposed (with non-solid colored markers) in panels A to C for comparison with the assorted pre- and post-exposure assay designs in A) 10 ug/mL, B) 15 ug/mL, and C) 20 ug/mL CAE with survival plots normalized to no extract/ no Pb (control). D) Recorded hour of first and last deaths, and hour death reached 50% within the 24-hour observation period (nr = did not reach 50% death)

Journal of Environment Pollution and Human Health. 2015, 3(1), 18-23 doi:10.12691/jephh-3-1-4