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From

Functional and Antioxidant Properties of Protein Hydrolysates from Grey Triggerfish Muscle and in vivo Evaluation of Hypoglycemic and Hypolipidemic Activities

Rayda Siala, Abdelmajid Khabir, Imen Lassoued, Ola Abdelhedi, Abdelfattah Elfeki, Tatiana Vallaeys, Moncef Nasri

Journal of Applied & Environmental Microbiology. 2016, 4(6), 105-119 doi:10.12691/jaem-4-6-1
  • Figure 1. Flow sheet for preparation of BPHs
  • Figure 2. Hydrolysis curves of Baliste capriscus muscle proteins treated with crude enzymes extracted from Zebra blenny viscera (BCPH-Z), sardinelle viscera (BPH-S) and A21 (BCPH-A21)
  • Figure 3. Solubility profiles of BCPHs as a function of pH obtained by treatment with crude enzymes extracted from Z.blenny viscera (BCPH-Z), sardinelle viscera (BCPH-S) and A21 (BPH-A21)
  • Figure 4. Scavenging effect on DPPH. free radical (A), antioxidant β-carotene bleaching method (B), reducing power (C) and metal chelating activity (D) of BPHs at different concentrations. BHA (2.0 mM; 0.36 mg/mL) or EDTA (1 mg/mL) was used as positive controls. Values presented are the mean of triplicate analyses
  • Figure 5. Hydroxyl radical-scavenging activity of BPHs at different concentrations (a) and gel electrophoresis pattern of the plasmid pCRII™TOPO incubated with Fenton's reagent in the presence and absence of BCPHs (b). Lane 1: untreated control: native pCRII™TOPO DNA (0.5 μg); lane 2: DNA sample incubated with Fenton's reagent; lanes 3, 4, and 5: Fenton's reagent+DNA+2 mg BPHs, BPH-Z, BPH-A21 and BPH-S, respectively
  • Figure 6. Inhibitory effects of BCPHs on α-amylase activity in the serum of surviving diabetic rats. Values are given as mean ±S.D. for 4 rats in each group. Values are statistically Presented as follows: a p < 0.05 significant difference compared to controls, b p < 0.05 significant difference compared to diabetic rats, cp< 0.05 significant difference compared to diabetic rats treated with acarbose
  • Figure 7. Effects of BCPHs on the blood glucose (A) and HbA1c (B) level of diabetic rats. Values are given as mean ± S.D. for 4 rats in each group. Values differ significantly at p < 0.05. Statistical analysis as in the legend of Figure 6
  • Figure 8. Serum lipase activity of control and experimental groups of rats. Values are given as mean ±SD for 4 rats in each group. Values differ significantly at p <0.05. Statistical analysis as in the legend of Figure 6
  • Figure 9. Histopathology of the livers (a) and pancreatic islets (b) of control and experimental animals