Figure 3. DGGE profiles of ammonia-oxidizing bacteria of the β-subclass of the Proteobacteria, obtained by nested PCR on July 2012, September 2012, and September 2013 samples. Lanes 1 to 18 correspond to the 18 different sampling stations; lanes “WW in” and “WW out” represent samples from domestic wastewater treatment plants before and after treatment. Lanes WM, weight markers. The principal bands observed are labeled with capital letters (A to L). All these bands have been cut and sequenced to determine the phylogeny of each ammonia-oxidizing bacterium and to compare the three sampling dates. The denaturing gradient is the same for the three DGGE gels; only 36.5 to 51.5% is shown here


Exploited Application of Denaturing Gradient Gel Electrophoresis in Analysis of Ammonia Oxidizing Bacterial Community Structure

Maulin P Shah

Journal of Applied & Environmental Microbiology. 2014, 2(4), 116-127 doi:10.12691/jaem-2-4-5