Isolation and Identification of Bacteria Associated with Fresh and Smoked Fish (Clarias gariepinus) In Minna Metropolis, Niger State. Nigeria
1Department of Biology Faculty of Natural Science Ibrahim Badamasi Babangida University Lapai. Niger State. Nigeria
2Department of Microbiology Faculty of Natural Science Ibrahim Badamasi Babangida University Mlapai. Niger State. Nigeria
An in-vitro assay was conducted to ascertain and identify major bacterial contaminants of fish, which hitherto had constituted an imported dietary intake of the people of Minna Metropolis, Nigeria. Fresh and smoked fish samples were collected from three different markets, the bacterial load of the samples was determined using the pour plate method. Identification and characterization of various isolates were based on gram-staining technique and biochemical tests. Clarias gariepinus was used for the study. In-vitro assay result revealed that the samples were contaminated by six bacteria species, which include Staphilococcus aureus, Bacillus subtilis, Staphilococcus epidermis, Salmonella epidermis, Salmonella typhii, Streptococcus spp. and Shigella sp. The mean bacterial load for the fresh fish was 1.84 x 106 cfu/ ml. and for the smoked fish 2.06 x 106 cfu/ ml.
Keywords: Clarias gariepinus, bacterial load, fresh fish, smoked fish, Minna metropolis
Journal of Applied & Environmental Microbiology, 2014 2 (3),
Received January 18, 2014; Revised March 29, 2014; Accepted March 31, 2014Copyright © 2013 Science and Education Publishing. All Rights Reserved.
Cite this article:
- U., Ibrahim B., BABA J., and Sheshi M. S.. "Isolation and Identification of Bacteria Associated with Fresh and Smoked Fish (Clarias gariepinus) In Minna Metropolis, Niger State. Nigeria." Journal of Applied & Environmental Microbiology 2.3 (2014): 81-85.
- U., I. B. , J., B. , & S., S. M. (2014). Isolation and Identification of Bacteria Associated with Fresh and Smoked Fish (Clarias gariepinus) In Minna Metropolis, Niger State. Nigeria. Journal of Applied & Environmental Microbiology, 2(3), 81-85.
- U., Ibrahim B., BABA J., and Sheshi M. S.. "Isolation and Identification of Bacteria Associated with Fresh and Smoked Fish (Clarias gariepinus) In Minna Metropolis, Niger State. Nigeria." Journal of Applied & Environmental Microbiology 2, no. 3 (2014): 81-85.
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The importance of fish cannot be overemphasized. Fish is a low fat food, a great source of protein, vitamins and minerals. Over the years, agriculture has gained a rapid interest due to the importance of fish as a cheap source of protein, since beef meat and even goat meat are beyond the reach of an average Nigerian citizen (Fang et. al., 2010). Fish constitute about 45% of the total amount of protein (FDF, 2007), even for a long time to come, this trend will continue. In Nigeria, the demand for fish greatly exceeds supply. This problem is aggravated by the low level of domestic fish production against the increase human production (Adeleke, 1999). As a result of the crucial role played by fish production in meeting the protein demand, information on the parasites of fish becomes particularly important, as they are known to significantly affect yield in fisheries (Hundson et. al., 2005).
Fish parasites may be grouped broadly as bacteria, fungi, virus e.t.c. These parasites put together are responsible for about 45% loses in fish farms (Kabata, 2008).
One of the earliest reports of fish parasites in Nigeria, was that of Awachie (1996) on the parasite of fish in the area of Kainji Reservoir, with 30% of Sarotherodon niloticus infected by acanthocephalan, and 9% of Clarias gariepinus infected by cestodes. In Eastern Nigeria, Jonah (1990), carried out a survey of Helminthes parasites of fish in Imo River, and recorded a low 7.7% level of infection. Robert (1999), reported that intensification of pond fish culture favour increase of disease agents and disease outbreak. Kabata (2008), also reported that pathological conditions arising from parasitic infection posed a serious consequences especially under a crowded conditions.
In various ways, fish could be contaminated by micro organisms. The aim of the present study is to determined and identify the bacterial pathogens contaminating fresh and smoked fish in Minna metropolis, Niger State.
2. Materials and Methods2.1. Study Area
The study was conducted in Minna metropolis, Niger State, located within longitude 633'E and latitude 93'N, with a land mass of 88 Km2. The vegetation of the area reflect that of Savannah zone, dominated by grass with scattered trees species. The climate presents two distinct seasons, a rainy season between April and October, and, a dry season (November-March) completely devoid of rain.2.2. Collection of Fish Samples
Fresh and dried fish samples were obtained from three different markets within Minna metropolis (Kure Ultra modern market, Tunga Goro market and Chanchaga market), between 7.00 a.m. and 8.00 a.m. local time. They were packed in a leather bag and transferred to the laboratory for identification and biological assays.
3. Sample Preparation3.1. Preparation of Serial Dilution
Sample preparation was made using the method described by Obi and Krakowiaka (1983). The part of the fresh fish body were scraped and swab stick was used to swab the fish body and inserted into the first test tube containing 9 ml of distilled water as a stock, and five other test tubes also containing 9 ml of distilled water were arranged serially in the test tube rack. 1 ml. of the stock was collected using a pipette to the first test tube and from the first test tube to the second test tube up to the fifth test tube respectively i.e. 10-1, 10-2, 10-3, 10-4 and 10-5 respectively. 10-4 and 10-5 were used as the dilution factor and 1 ml. was taken from each factor into a sterilized petri dish in duplicate. All plates were incubated at a temperature of 37C for 24 hrs, before colony counting and isolation procedures.3.2. Media Preparation
Nutrient agar was prepared by weighing 28 g and dissolved in 1 litre of distilled water. The dissolved nutrient agar was then autoclaved at a temperature of 121C for 15 minutes. The media was allowed to cool down and pour into each of the 8 petri dishes containing 1 ml. of the diluents. It was then allowed to solidify and incubate at temperature of 37C for 24 hrs.3.3. Bacteria Colony Count
Bacteria colonies were counted using colony machine. The number of colonies on the plate was multiplied by the reciprocal of the dilution factor and calculation was done for 1 ml of original sample, and plating was done in duplicate foe each dilution. An average count was taken to obtain the total count.3.4. Identification and Characterization of the Isolates
All isolates were sub-cultured to obtain a pure culture and a gram-staining carried out. Identification of the isolates was carried out based on the method described by Sakazaki and Shimad (1986), Collins et. al.,(1989) and Cheesebrough (2002).
Bacterial colony count of fresh fish (Clarias gariepinus) from the three markets above revealed that samples from Chanchaga market, Minna metropolis has the highest number of Bacteria load, and the following species of bacteria were identified from the samples from Chanchaga market S. aureus, B. subtilis, Shigella sp., S. epidermis and S. typhii. It also has the highest number of species identified, while Kure ultra modern market and Tunga Goro market has three species identified from each of them as follows respectively S. aureus, B. subtilis, Shigella sp., and, S. epidermis, Shigella sp., B. subtilis.
Bacterial colony count of smoked fish (Clarias gariepinus) from the three markets sampled above in Minna metropolis, Niger State, revealed that samples from Chanchaga has the highest number of bacterial load, similar to what was obtained for fresh fish in Table 1. Chanchaga market and Kure ultra modern market recorded the following four bacteria species identified from the smoked sampled fishes collected S. aureus, B. subtilis, Shigella sp. and S. epidermis. While Tunga Goro market has three bacteria species identified i.e. S. aureus, B. subtilis and Shigella sp. from the smoked sample fishes collected.
Table 3. The result of the biochemical and identification of bacterial isolate of the smoked fish specimens (Clarias gariepinus)
Table 4. The result of the biochemical and identification of bacterial isolate of the fresh fish specimens (Clarias gariepinus)
The study revealed a mean bacteria count value of 1.84 x 106 cfu/ml. for the fresh fish and 2.06 x 106 cfu/ml. for the smoked fish. The bacteria count for the fresh fish ranged from 1.02 x 106-2.32 x 106 cfu/ml. which is less than that of smoked fish that ranged from 1.85 x 106-2.41 x 106 cfu/ml. The mean and the range of fresh samples is less than those of smoked fish. This could be due to sanitary conditions under which the smoked fish samples are handled and kept (Tiamiyu et. al., 2011).
The result of this study revealed that Staphylococcus aureus, Shigella spp., Staphylococcus epidermis, Bacillus subtilis were the common pathogenic bacteria found associated with fresh and smoked fish in Minna metropolis. The presence of S. aureus was attributed to the contamination of the fish samples by man through handling and processing. Clucas and Ward (1996), recorded S. aureus, but stated that if ever, it seldom occurs as natural microflora of fish and shellfish. Its main habitat is humans and animals, and found mostly in the nose, throat and skin of healthy individuals (Clucas and Ward, 1996). This indicates that fresh and smoked fish with this bacteria pathogens, must have been contaminated through handling during post harvest. In a similar study carried out by Moshood and TengkuHaziyamin (2012), Bacillus aureus, Staphylococcus aureus, protens mirabilis, Klebsiella sp., Salmonella typhii and Streptococcus sp. were all found to be associated with smoked fish. It was suspected that these organisms may have contaminated the smoked fish through human handlers, air and soil. The findings of Moshood et. al., 2012 corroborates the findings in this study, since common bacteria such as Staphylococcus aureu, Salmonella typhii and Bacillus subtilis were also isolated. The presence of these organisms in the smoked fish samples of Clarias gariepinus might be due to increase in moisture content of the product during storage, and also increase in temperature that favours the growth of these organisms. During handling of fish, the natural flora of fish environment will be contaminated with organisms associated with man, such as Salmonella typhii and Staphylococcus aureu, both isolated in this investigation, can grow well at 30C - 37C (Brown, 2004). In a related development, Tiamiyu et. al., 2011, isolated and identified Staphylococcus aureu, Bacillus sp., Salmonella sp. and Streptococcus sp. from the skin of Clarias gariepinus also supports the outcome of this study. Salmonella sp. may be present naturally in tropical aquatic environments (Tiamiyu et. al., 2011). It is well established that aquatic birds spread Salmonella Sp. and other pathogen in the environment (Fenion, 1983; Beveridge, 1989). Bacillus sp., Escherichia coli, Salmonella sp., Streptococcus sp. and S. aureus have been implicated in fish-borne diseases of humans (Babu, 2000). Ikpi and Offem (2011) in a study carried out on bacterial infection of mudfish Clarias gariepinus isolated and identified Staphylococcus aureu, E. coli and Pseudomonas fluorescens however dominating. Staphylococcus epidermis, one of the microorganism isolated in this study was among the predominant microorganisms isolated from both gills and skin of Clarias gariepinus in a study conducted by Hassan et. al., (2010). In support of Samonella typhii that was isolated in this study, Salmonella sp. have been recovered from gills, intestine and whole body of catfish Clarias gariepinus and sea food in Malaysia (Bundiati et. al., 2011; Bremer et. al., 2003; Kumar et. al., 2009; Heinitz et. al., 2000; and Ponce et. al., 2008). Also, in a study conducted by Efuntoye et. al., (2012), Salmonella typhii and Salmonella entridis were isolated among other organisms. This constitutes a food safety problem, because catfish could be a potential agent of transfer of these species to unsuspecting customers.
The pathogenic state of species of streptococcus is alarming. For instance, Streptococcus parauberis has become important disease agent in the aqua culture industries of North East Asia (Korea, Japan and China), most especially among olive flounder aqua culture farms (Seong et. al., 2013). Only recently, Nho et. al., (2009), reported that S. parauberis is the dominant etiologicalagent of Streptococcus characterized y clinical symptoms, such as chronic wasting syndrome, heamorrhagic septicaemia, exophtaimia and meningitis with abnormal swimming. Streptococcal diseases have been reported worldwide in wild and farmed populations of diverse fresh water and marine fish (Austin and Austin, 1993, Kusuda and Salati, 1993). In 1993, there was an important epizootic outbreak of Streptococcosis in turbot Scophthalmus maximum cultured in Galacia (NW Spain), that was initially thought to be caused by an Enterococcus species-like bacterium (Toranzo et. al., 1994). The disease has been the main limiting factor of the turbot culture in spain (Toranzo et. al., 1995, Romalde et. al., 1996). Other Streptococcus sp. that have been found to be associated with aquatic contamination include Streptococcus pyogenes, Streptococcus pneumonia etc.
The public health importance of bacterial flora of Nigeria fish species have not been adequately defined due mainly to mode of food preparation in the tropics, which involved cooking for considerable length of time. The heat would have eliminated most, if not all the bacterial flora (Sowunmi et. al., 2008).
It is noteworthy that sanitary condition under which fishes are handled, processed and stored be improved upon to reflect standard or good practices.
|||Adeleke, I. (1999). Fish production in Nigeria. Sunday Punch, May, 1999. PP. 29.|
|||Austin, B. and Austin, D. B. (1993). Bacterial fish pathogen. Disease in farmed and wild fish. 2nd edn. Ellis Horwood, Chichester.|
|||Brown, G. E. A. (2004). A Report on the prevalence of Bacteria specie in Retail Smoked fish within Bauchi Metropolis.|
|||Budiati, T., Rusul, G., Alkarkhi, A. F. M., Ahmad, R. and Arip, Y. T. (2011). Prevalene of Salmonella Spp. From catfish (Clarias gaiepinus) by using improvement isolation methods. International conference. Asia Agric. Animal Animal IPCBEE, 13: 71-75.|
|||Bremer, P. J., Fletcher, G. C. and Osborne, C. (2003). Salmonella in Seafood, New Zealand Institute for Crop and Food Research Ltd., New Zealand.|
|||Beveridge, M. C. M., (1989). Problems caused by birds at inland waters and freswater fish farms: In: Welcomme, R. ed. Report of the EIFAC working party on prevetion and control of birds predation in aquaculture and fisheries, Rome, Food and Agriculture Organisation of the United Nations, 34-73 (EIFAC Technical Paper 51).|
|||Babu, P. S. (2000). Ichyozoonoses. Fish farmer International, 14: 14-17.|
|||Cheesbrough, M. (2002). District Laboratory Practice in Tropical Countries. (Part II). Tropical Health Technology Publishers, Great Britain. 40-56 pp.|
|||Claucas, I. J. and Ward, A. R. (1996). Post-harvest Fisheries Development: A Guide to Handling, Preservation, Processing and Quality. Chartan Maritime, Kent. ME4 TB,United Kingdom.|
|||Collins, C. H., Lyne, P. M. and Grange, G. M. (1989). Microbiological Methods. 6th Ed. Butterwoths. London. 56-66 pp.|
|||Efuntoye, M. O., Olurin and Jegede, G. C. (2011). Bacterial Flora from Healthy Clarias gariepinus and their antimicrobial resistance pattern. Advance Jour. of food Science and Technology 4 (3): 121-125.|
|||Fang, X., Guo, X. and Yu, J. (2010). The preliminary studies on the beterotrophic bacteria in high yield fish ponds. Fish journal 13 (2). 66-71.|
|||FDF (2007). Federal Department of Fisheries. Fisheries statistics of Nigeria, 4th Ed.: 1995-2007. 49 pp.|
|||Fenion, D. R. (1983). A comparison of Salmonella serotypes found in the faeces of of gulls feeding at sewage works with serotypes in sewage. Journal of Hygiene., 91: 47-52.|
|||Hassan, I. E., Viola, H. Z., Abdallah, M. E. and Dinna, A. E. (2010). Studies on the effects of bacterial diseases on skin and gill structure of Clarias gariepinus in Dakahlia Province, Egypt. Annals of Biological Research. 1 (4): 106-118. Fish jour.|
|||Heinitz, M. L., Ruble, R. D., Wagner, D. E. and Tatini, (2000). Incidence of Salmonella in fish and sea food. Int. Jour. Of Food Microbiol., 63: 579-592.|
|||Hudson, Q., akennet, A. A. and Meyer, R. (2005). Survey of specific fish pathogens in Devisl fish pond, North Dakota, Journal of aquatic animals 6 pp., 12-99 pp.|
|||Ipki, G. U. and Offem, B. O. (2008). Bacterial infection of cultural fishes in the fish farm of the Cross River University of Technology. Egypt Jour. Of Microbiology. 21: 57-63.|
|||Jonah, T. U. (1990). Some emergent diseases and management problems of Orecromis niloticus, Sarotherodon galileus and Clarias sp. in Nigeria. Jour. of Aquatic Science. 2: 153-161|
|||Kabata, Z. (2008). Parasites and Diseases of Fish in the Tropics. Taylor Francis publ. london. 92 pp.|
|||Kumar, R., Surendran, P. K. and Tampuran, N. (2009). Distribution and genotypic characteristics of Salmonella serovars isolated from tropical sea food of Cochin, India J. Appl. Microbiol., 106: 515-524.|
|||Kusuda, R. and Salati, F. (1993). Major bacterial diseases affecting mariculture in Japan. Annu. Rev. Fish Dis. 3: 69-85.|
|||Moshood, A. Y. and TengkuHaziyamin, A. A. (2012). Isolation and Identification of Bcateria in Retailed Smoked Fish within Bauchi Metropolis. Jour. of Pharm. And Biol. Sciences. 3 (1): 1-5.|
|||Nho, S. W. Shin, G. W., park, S. B., Jang, H. B. and Cha, I. S. (2009). Phenotypic characteristic of Streptococcus iniae and Streptococcus parauberis isolated from olive flounder (Paralichthys olivaceus). FEM Microbiol Lett 293 (1): 20-27.|
|||Obi, S. K. C. and Krakowiaka, A. (1983). Theory and practices of food Microbiology. (Unpublish manual).|
|||Ponce, E., Khan, A. A., Cheng, C. M., Sumange-West, C. and Cerniglia, C. E. (2008). Prevalence and Characterisation of Salmonella enterica serovar Welevreden from imported sea food. Food Microbiol. 1: 29-35.|
|||Robert, R. J. (2001). The pathophysiology and systemic pathology of Teleost. In: Fish pathology Roberts (Ed.). Ballure Tindal. London. 56-134 pp.|
|||Romalde, J. L., Magarinos, B., Nunez, S., Ba rja, J. L. and Toranzo, A. E. (1996). Host range susceptibilityof Enterococcus sp. strains isolated from diseased turbot: possible routes of infection. Appl. Environ. Microbiol. 62: 607-611.|
|||Seong-Won, N., Jun-ichi, H., Seong, B. P., Ho, B. J., In Seok, C., Motoshige, Y., Yoji, N., Atsushi, F., Motohiko, S., Kinya, K., Hidehiro, K., Ikuo, H., Haruko, T., Takashi, A. and Tae-Sung, J. (2013). Comparative Genomic Characterisation of Three Streptococcus parauberis Strains in Fish Pathogen, as Assessed by Wide-Genome Analyses. Retrieved from http: www. Plosone.org/article/info%3Adoi%F10.1371%2Fjournal.pone.0080395 on 26/03/2014.|
|||Sowunmi, A. A., Okunubi, M. A. and Efuntoye, M. O. (2008). Occurrence of bacteria in gill and buccal cavity of Clarias gariepinus (Burchell, 1822) and Tilapia zilli (Gervais) from Lekki lagoon, Southwest, Nigeria. Advance Jour. of food Science and Technology 4 (3): 121-125.|
|||Sakazaki, R. and Shimad, T. (1986). Vibro species as causative agent of food-borne infection. In: Development of Food Microbiology. Roinson, R. K. London Elsevier, 2: 123-151.|
|||Tiamiyu, A. M., Emikpe, B. O. and Adedeji, O. B. (2011). Isolation and Identification of aerobic bacteria flora of the skin and stomach of wild and cultured Clarias gariepinus and Oreochromis niloticus from Ibadan, Southwest, Nigeria. Journal of Applied Sciences Research, 7 (7): 1047-1051.|
|||Toranzo, A. E., Devesa, S., Heinen, P., Riaza, A., Nunez, S., Barja, J. L., (1994). Streptococcosis in cultured turbot caused by an Enterococcus-like bacterium. Bull. Eur. Assoc. Fish Pathol. 14:19-23.|
|||Toranzo, A. E., Cutrin, J. M., Nunez, S., Romalde, J. L. and Barja, J. L., (1995). Antigenic characterization of Enterococcus strains pathogenic for turbot and their relationship with other Gram positive bacteria. Dis. Aquat. Org. 21: 187-191|
|||Ugwuor, N. G., Anadu, D. L., and Ejike, C.(1997).Pseudomonas infection of catfish of the genus Clarias gariepinus (Burchell, 1982). Journal of aquatic science. 5: 11-13.|