Caries is an infectious diseases of the oral cavity in which oral biofilms play a causative role. Moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and drugs acting on biofilm to, due to their widespread presence and accessibility. The aim of the present in vitro study was to investigate the efficacy of specific fluoridated toothpaste on the biofilms formed by the test microorganisms (Streptococcus mutans, Facklamia homins and Streptococcus saliviurus). The colonizations of the three test bacteria on four surfaces (two natural; permenant and deciduous tooth surfaces and two artificial ;composite and amalgam filling surfaces) were observed with scanning electron microscopy (SEM).SEM detected the anti-biofilm effects on the same four surfaces medicated with a tooth paste. Results: The used tooth paste declined the formed biofilms on four surfaces efficiently. The natural tooth surfaces responded in same manner to the tooth paste while composite responded more efficiently than amlagam surfaces. Conclusions: Flourdated toothpaste (0.454%) effectively can remove formed biofilms of the three test bacteria on natural surfaces and composite filling surfaces. It removed formed biofilm on amalgam surfaces but not as from other three surfaces.
| [1] | Lamont, R.J. Oral Microbiology and Immunology; ASM Press: Washington, DC, USA, 2006.View Article |
| [2] | Marit Øilo and Vidar Bakken. Biofilm and Dental Biomaterials, Materials 2015, 8, 2887-2900.View Article PubMed |
| [3] | Beighton D: The complex oral microflora ofhigh-risk individuals and groups and its role in the caries process. Community Dent Oral Epidemiol 2005; 33: 248-255View Article PubMed |
| [4] | R. J. Fitzgerald and P. H. Keyes, “Demonstration of the etiologic role of streptococci in experimental caries in the hamster,” The Journal of the American Dental Association, 1960, vol. 61, pp. 9-19.View Article PubMed |
| [5] | Kolenbrander PE, Palmer RJ Jr, Periasamy S, Jakubovics NS.Oral multispecies biofilm development and the key role of cell-cell distance. Nat Rev Microbiol. 2010; 8: 471-80View Article PubMed |
| [6] | Gamboa F, Estupinan M, Galindo A. Presence of Streptococcus mutans in saliva and its relationship with dental caries: Antimicrobial susceptibility of the isolates. Universitas Scientiarum. 2004; 9(2): 23-7.View Article |
| [7] | Regos J, Hitz HR. Investigations on the mode of action of triclosan, a broad-spectrum antimicrobial agent. Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 1 Orig. Reihe A 1974; 226: 390-401.View Article |
| [8] | Degiam Z.D., An in Vitro Antimicrobial activity of six commercial toothpastes, This-qar. Med. J. (TQMJ), 2010, 4(4), 127-133.View Article |
| [9] | Marinho V, Higgins J, Logan S, Sheiham A. Fluoride toothpastes for preventing dental caries in children and adolescents. Cochrane Database of Systematic Reviews 2003; Issue 1.View Article |
| [10] | Australian Government, National Health and Medical Research Council. A Systematic Review of the Efficacy and Safety of Fluoridation, 2007. |
| [11] | Maria Ange´ Lica Hubb DeMenezes Oliveria.Carolina Paestorres, Jaciaramiranda Gomes-Silva, Michelle Alexandra Chinelatti, Fernando Carlos Hueb De Menezes, Regina Gueika Palma-Dibb and Maria Cristina Borsaatto,Microstructure and Mineral Composition of Dental Enamel of Permanent and Deciduous Teeth microscopy research and technique 2010, 73:572-577. |
| [12] | Gavin, P. J., J. R. Warren, A. A. Obias, S. M. Collins, and L. R. Peterson. 2002. Evaluation of the Vitek 2 system for rapid identification of clinical isolates of gram-negative bacilli and members of the family streptococcaceae. Eur. J. Clin. Microbiol. Infect. Dis. 21: 869-874. PubMed |
| [13] | Hope CK, Burnside G, Chan SN, Giles LH, Jarad FD. Validation of an extracted tooth model of endodontic irrigation. J Microbiol Methods 2011; 84: 131-3.View Article PubMed |
| [14] | Abd El-Bakey R.M. Application of Scan Electron Microscope for the Morphological Study Of Biofilm in Medical Devices, Microbiology Department ,Faculty of Pharmacy, Minia University, Egypt; 201230: 591-616. |
| [15] | Clinical and laboratory standards institutes. Performance standard for antimicrobial susceptibility testing,Seventeenth informational supp. 2007, M100-S17, PA). |
| [16] | Addy M. Chemical plaque control. In: Kieser BJ, editor. Periodontics: a practical approach. London: Wright Publishing Company; 1990. pp. 527-34. |
| [17] | Menendez A, Li F, Michalek SM, Kirk K, Makhija SK, Childers NK. Comparative analysis of the antibacterial effects of combined mouthrinses on Streptococcus mutans. Oral Microbiol Immunol. 2005; 20(1): 31-4.View Article PubMed |
| [18] | Sanz M. Serrano J. Iniesta M. Santa Cruz I. Herrera D. Antiplaque and Antigingivitis Toothpastes Karger, 2013, vol 23, pp 27-44.View Article |
| [19] | Buzalaf MA, Pessan JP, Honório HM, ten Cate JM. Mechanisms of action of fluoride for caries control. Monogr Oral Sci 2011; 22: 97-114.View Article PubMed |
| [20] | Bradshaw DJ, Marsh PD, Hodgson RJ, Visser JM. Effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms.2002 Caries research 36: 81-86.View Article PubMed |
| [21] | P.S. Watson, H.A. Pontefract, D.A. Devine1, R.C. Shore, B.R. Nattress, J. Kirkhamand C. Robinson. Penetration of Fluoride into Natural Plaque Biofilms J Dent Res 2005, 84(5): 451-455. PubMed |
| [22] | Bath-Balogh M, Fehrenbach MJ, Thomas P, Cmi. Illustrated Dental Embryology, Histology, And Anatomy. Saunders. 1997; 23(2): 44. |
| [23] | Scheid RC, Woelfel JB. Woelfel's dental anatomy: its relevance to dentistry. Wolters Kluwer/Lippincott Williams & Wilkins. 2007.View Article |
| [24] | De Menezes Oliveira MA, Torres CP, Gomes-Silva JM, Chinelatti MA, De Menezes FC, Palma-DibbRG, et al. Microstructure and mineral composition of dental enamel of permanent and deciduous teeth. Microscopy research and technique. 2010; 73(5): 572-7. PubMed |
| [25] | J. Olossn and B. Krasse. Method for studying adherence of oral streptococci to solid surfaces, European Journal of Oral Sciences1976, Vol 84, issue 2p. 20-28.View Article |
| [26] | Rokiewicz D, Daniluk T , Zaremba ML, Cylwik-Rokicka D, Łuczaj-Cepowicz E, Milewska R, Marczuk-Kolada G, Stokowska W Bacterial composition in the supragingival plaques of children with and without dental caries. Advances in Medical Sciences. 2006. Vol. 51. Suppl. 1.View Article |
| [27] | M. N. Bellon-Fontaine, N. Mozes, H. C. van der Mei, J. Sjollema, O. Cerf, P. G. Rouxhet and H. J. Busscher, “A Comparison of Thermodynamic Approaches to Predict the Adhesion of Dairy Microorganisms to Solid Sub-strata,” Cell Biophysics, Vol. 17, No. 1, 1990, pp. 93-106.View Article PubMed |
| [28] | H. J. Busscher, M. M. Cowan and H. C. van der Mei, “On the Relative Importance of Specific and Non-Specific Approaches to Oral Microbial Adhesion,” FEMS Micro-biology Reviews, Vol. 8, No. 3-4, 1992, pp. 199-209.View Article PubMed |
| [29] | Y. H. An and R. J. Friedman, “Concise review of mechanisms of bacterial adhesion to biomaterial surfaces,” Journal of Biomedical Materials Research, vol. 43, no. 3, pp. 338-348, 1998.View Article |
| [30] | B. Nyvad and O. Fejerskov, “Scanning electron microscopy of early microbial colonization of human enamel and root surfaces in vivo,” Scandinavian Journal of Dental Research, vol. 95, no. 4,pp. 287-296, 1987.View Article |
| [31] | J. Einwag, A.Ulrich and F. Gehring, “In-Vitro Plaque Accumulation on Different Filling Materials,” Oralprophylaxe, Vol. 12, No. 1, 1990, pp. 22-25. PubMed |
| [32] | L. Mei, H. J. Busscher, H. C. van der Mei and Y. Ren, “Influence of Surface Roughness on Streptococcal Adhe-sion Forces to Composite Resins,” Dental Materials, Vol. 27, No. 8, 2011, pp. 770-778.View Article PubMed |
| [33] | M. Yamauchi, K. Yamamoto, M. Wakabayashi and J. Kawano, “In Vitro Adherence of Microorganisms to Den-ture Base Resin with Different Surface Texture,” Dental Materials Journal, Vol. 9, No. 1, 1990, pp. 19-24.View Article PubMed |
| [34] | R. D. Fields and M. V. Lancaster, “Dual-attribute continuous monitoring of cell proliferation/cytotoxicity,” American Biotechnology Laboratory, vol. 11, no. 4, pp. 48-50, 1993. PubMed |
| [35] | Schierholz, J.M and Beuth, J.. Implant infections: a haven of opportunistic bacteria. J. Hosp. Infect., 2001 49: 87-93.View Article PubMed |
| [36] | John L.Pace,Mark E.Rupp and Roger G. Finch. (2005). Biofilm, Infection and Antimicrobial Therapy, Taylor and Franks Group; 43-45. PubMed |
| [37] | Bonner, M.C.; Tunney, M.M.; Jones, D.S. and Gorman, S.P. Factors affecting in-vitro adherence of ureteral stent biofilm isolates to polyurethane. Int. J. Pharmaceutics. 1997, 151: 201-207.View Article |
| [38] | Jansen, B.; Peters, G. and Pulverer, G.. Mechanisms and clinical relevance of bacterial adhesion to polymers. J. Biomatr. Appl.1988, 2: 520-543.View Article PubMed |
| [39] | A. T. Poortinga, R. Bos and H. J. Busscher, “Measure-ment of Charge Transfer during Bacterial Adhesion to an Indium Tin Oxide Surface in a Parallel Plate Flow Cham-ber,” Journal of Microbiological Methods, Vol. 38, No. 3, 1999.View Article |
| [40] | L. Mei, H. C. Van der Mei, Y. Ren, W. Norde and H. J. Busscher, “Poisson Analysis of Streptococcal Bond Strengthening on Stainless Steel with and without a Sali-vary Conditioning Film,” Langmuir, Vol. 25, No. 11, 2009, pp. 6227-6231.View Article PubMed |
| [41] | A. Leonhardtt, J. Olsson2, and G. Dahlen. Bacterial Colonization on Titanium, Hydroxyapatite, and Amalgam Surfaces in vivoJ Dent Res 1995, 74(9): 1607-1612.View Article |
| [42] | Glassman MD, Miller IJ. Antibacterial properties of one conventional and three high-copper dental amalgams. J Prosthet Dent 1984, 52: 199-20350-51View Article |
| [43] | D. Ready, J. Pratten, N. Mordan, E. Watts and M. Wilson, “The Effect of Amalgam Exposure on Mercury- and An-tibiotic-Resistant Bacteria,” International Journal of An-timicrobial Agents, Vol. 30, No. 1, 2007, pp. 34-39.View Article PubMed |
| [44] | Bernardo, M; Martin, MD; Lerouz, BG. “Survival and reasons for failure of amalgam versus resin-based composites posterior restorations placed in a randomized clinical trial”. The Journal of the American Dental Association. 2007, 138: 775-78.View Article PubMed |
| [45] | C. Hansel, G. Leyhausen, U. E. Mai and W. Geurtsen, “Effects of Various Resin Composite (Co)monomers and Extracts on Two Caries-Associated Micro-Organisms in Vitro,” Journal of Dental Research, Vol. 77, No. 1, 1998, pp. 60-76.View Article PubMed |
