Figure 6. Image correlation spectroscopy [25]. (A) Several frames of time image series. (B) Spatial ICS to obtain the point spread function of LSM (see text). The raw spatial autocorrelation function of the 1st frame of the image series is denoted by the grey surface, and the fitted 2D Gaussian function is denoted by the grey mesh. The point spread function, i.e., beam radius of LSM, is obtained by the fitting. (C) Intensity at a pixel fluctuates with number of frames. (D) Temporal ICS to obtain the diffusion time. A temporal image autocorrelation function (open circles) was derived from the time image series. The temporal autocorrelation function was fitted to a simple one component 2D model (solid line) which can be derived from the diffusion equation

From

Fluorescence Correlation Analysis for Diagnosis Based on Molecular Dynamics

Yasutomo Nomura

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