Background: Diabetes is responsible for rapidly increasing morbidity globally such that it has been listed among the four priority non-communicable diseases. Global prevalence of diabetes was 8.5% of the adult population by 2014 but is steadily rising. It is estimated that global prevalence of diabetes will be 472 million by 2030 with diabetic neuropathy affecting up to 236 million people. Newer interventions based on natural compounds are required since the available options are marred with diverse side effects. Plants’ natural bioactive compounds are capable of preventing development of diabetic complications via different mechanisms making them potential alternatives for its management. Kalanchoe pinnata and Bidens pilosa have been used in folkloric medicine to treat diseases including diabetes. Objective: Our study aimed at determining phytochemicals present in these two plants and their potential for use in management of diabetes. Material and Methods: Extracts from the two plants were prepared by maceration in different solvents followed by determination of presence of ten phytochemicals. Results and Discussion: Different polyphenolic compounds, glycosides and saponins were detected in aqueous extracts of both plants. Higher concentrations of flavonoids and phenolic acids were detected in aqueous extracts from B. pilosa (30.11±0.2 mg of QE/100 g and 92.7±0.1 mg of GAE/100 g) compared to K. pinnata. Conclusion: The presence of these phytochemicals qualify these two plants as candidates for development of interventions for managing type 2 diabetes.
| [1] | Ayah R, Joshi MD, Wanjiru R, Njau EK, Otieno CF, Njeru EK & Mutai KK. A population-based survey of prevalence of diabetes and correlates in an urban slum community in Nairobi, Kenya. BMC Public Health, 2013; 13:371.View Article PubMed |
| [2] | WHO. Global report on diabetes. World Health Organization, 2016. |
| [3] | Levitt NS. Diabetes in Africa: epidemiology, management and healthcare challenges. Heart, 2008; 94(11): 1376-1382.View Article PubMed |
| [4] | Christensen DL, Friis H, Mwaniki DL, Kilonzo B, Tetens I, Boit MK et al. Prevalence of glucose intolerance and associated risk factors in rural and urban populations of different ethnic groups in Kenya. Diabetes Res Clin Pract, 2009; 84(3): 303-310.View Article PubMed |
| [5] | Pandey A, Chawla S & Guchhait P. Type-2 diabetes: Current understanding and future perspectives. IUBMB Life, 2015; 67(7): 506-513.View Article PubMed |
| [6] | Inzucchi SE & Majumdar SK. Current Therapies for the Medical Management of Diabetes. Obstet Gynecol, 2016; 127(4): 780-794.View Article PubMed |
| [7] | Olokoba AB, Obateru OA & Olokoba LB. Type 2 diabetes mellitus: a review of current trends. Oman Med J, 2012; 27(4): 269-273.View Article PubMed |
| [8] | Alam U, Asghar O, Azmi S & Malik RA. General aspects of diabetes mellitus. Handb Clin Neurol, 2014; 126: 211-222.View Article PubMed |
| [9] | Yan LJ. Redox imbalance stress in diabetes mellitus: Role of the polyol pathway. Animal Model Exp Med, 2018; 1(1): 7-13.View Article PubMed |
| [10] | Kashihara N, Haruna Y, Kondeti VK & Kanwar YS. Oxidative stress in diabetic nephropathy. Curr Med Chem, 2010; 17(34): 4256-4269.View Article PubMed |
| [11] | Verpoorte R, Choi YH & Kim HK. Ethnopharmacology and systems biology: a perfect holistic match. J Ethnopharmacol, 2005; 100(1-2): 53-56.View Article PubMed |
| [12] | Saeed N, Khan MR & Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med, 2012; 12: 221.View Article PubMed |
| [13] | Nostro A, Germanò MP, D’angelo V, Marino A & Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol, 2000; 30(5): 379-384.View Article PubMed |
| [14] | Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, et al. Phytochemical Analysis of Medicinal Plants Occurring in Local Area of Mardan. Biochemistry & Analytical Biochemistry, 2013; 2(4).View Article |
| [15] | Chhikara N, Devi HR, Jaglan S, Sharma P, Gupta P & Panghal A. Bioactive compounds, food applications and health benefits of Parkia speciosa (stinky beans): a review. Agric & Food Secur, 2018; 7(1): 46.View Article |
| [16] | Krishnaiah D, Sarbatly R & Bono A. Phytochemical antioxidants for health and medicine - A move towards nature. BMBR, 2007; 1(14): 97-107. |
| [17] | Firdous SM. Phytochemicals for treatment of diabetes. EXCLI J, 2014; 13: 451-453. PubMed PubMed |
| [18] | Singh R, Kaur N, Kishore L & Gupta GK. Management of diabetic complications: a chemical constituents based approach. J Ethnopharmacol, 2013; 150(1): 51-70.View Article PubMed |
| [19] | Sefi M, Fetoui H, Makni M & Zeghal N. Mitigating effects of antioxidant properties of Artemisia campestris leaf extract on hyperlipidemia, advanced glycation end products and oxidative stress in alloxan-induced diabetic rats. Food Chem Toxicol, 2010; 48(7): 1986-1993.View Article PubMed |
| [20] | Sharma B, Balomajumder C & Roy P. Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic rats. Food Chem Toxicol, 2008; 46(7): 2376-2383.View Article PubMed |
| [21] | Gulfraz M, Ahmad A, Asad MJ, Sadiq A, Afzal U, Imran M, et al, Antidiabetic activities of leaves and root extracts of Justicia adhatoda Linn against alloxan induced diabetes in rats. AJB, 2011; 10(32): 6101-6106. |
| [22] | Singh J & Kakkar P. Antihyperglycemic and antioxidant effect of Berberis aristata root extract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol, 2009; 123(1): 22-26.View Article PubMed |
| [23] | Dewanjee S, Das AK, Sahu R & Gangopadhyay M. Antidiabetic activity of Diospyros peregrina fruit: effect on hyperglycemia, hyperlipidemia and augmented oxidative stress in experimental type 2 diabetes. Food Chem Toxicol, 2009; 47(10): 2679-2685.View Article PubMed |
| [24] | Noh, H., & Ha, H. (). Reactive oxygen species and oxidative stress. Contrib Nephrol, 2011; 170: 102-112.View Article PubMed |
| [25] | Choi R, Kim BH, Naowaboot J, Lee MY, Hyun MR, Cho EJ, et al. Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes. EMM, 2011; 43(12): 676-683.View Article PubMed |
| [26] | Abdel-Raouf HS. Anatomical traits of some species of Kalanchoe (Crassulaceae) and their taxonomic value. Annals of Agricultural Sciences, 2012; 57(1): 73-79.View Article |
| [27] | Fernandes JM, Félix-Silva J, da Cunha LM, Gomes JAS, Siqueira EMS, Gimenes LP, et al., Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom. PLOS ONE, 2016; 11(12): e0168658.View Article PubMed |
| [28] | Cawich SO, Harnarayan P, Budhooram S, Bobb NJ, Islam S & Naraynsingh V. Wonder of Life (kalanchoe pinnata) leaves to treat diabetic foot infections in Trinidad & Tobago: a case control study. Trop Doct, 2014; 44(4): 209-213.View Article PubMed |
| [29] | Patil SB, Dongare VR, Kulkarni CR, Joglekar MM & Arvindekar AU. Antidiabetic activity of Kalanchoe pinnata in streptozotocin-induced diabetic rats by glucose independent insulin secretagogue action. Pharm Biol, 2013; 51(11): 1411-1418.View Article PubMed |
| [30] | Silva FL, Fischer DCH, Tavares JF, Silva MS, de Athayde-Filho PF & Barbosa-Filho JM. Compilation of secondary metabolites from Bidens pilosa L. Molecules, 2011; 16(2): 1070-1102.View Article PubMed |
| [31] | Bartolome AP, Villasenor IM & Yang WC. Bidens pilosa L. (Asteraceae): Botanical Properties, Traditional Uses, Phytochemistry, and Pharmacology’, Evidence-Based Complementary and Alternative Medicine, 2013. [Online].View Article |
| [32] | Redl K, Breu W, Davis B & Bauer R. Anti-inflammatory active polyacetylenes from Bidens campylotheca, Planta Med, 1994; 60(1): 58-62.View Article PubMed |
| [33] | Rybalchenko NP, Prykhodko VA, Nagorna SS, Volynets NN, Ostapchuk AN, Klochko VV, et al. In vitro antifungal activity of phenylheptatriyne from Bidens cernua L. against yeasts. Fitoterapia, 2010; 81(5): 336-338.View Article PubMed |
| [34] | Chien SC, Young PH, Hsu YJ, Chen CH, Tien YJ, Shiu SY, et al., Anti-diabetic properties of three common Bidens pilosa variants in Taiwan. Phytochemistry, 2009; 70(10): 1246-1254.View Article PubMed |
| [35] | Liang YC, Yang MT, Lin CJ, Chang CLT & Yang WC. Bidens pilosa and its active compound inhibit adipogenesis and lipid accumulation via down-modulation of the C/EBP and PPARγ pathways. Scientific Reports, 2016; 6: 24285.View Article PubMed |
| [36] | Ebbo AA, Mamman M, Suleiman MM, Ahmed A, and Bello A. Preliminary Phytochemical Screening of Diospyros Mespiliformis. Anat Physiol, 2014; 4(4): 1-3. |
| [37] | Samidha K, Vrushali K & Vijaya P. Estimation of Phenolic content, Flavonoid content, Antioxidant and Alpha amylase Inhibitory Activity of Marketed Polyherbal Formulation. JAPS, 2014; 4(9): 61-65. |
| [38] | Baba SA & Malik SA. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. JTUSCI, 2015; 9(4): 449-454.View Article |
| [39] | Ming-jun C, Xin Y, Yu-qing C & Chao Z. Phytochemicals for Non-insulin Diabetes Mellitus: A Minireview on Plant-Derived Compounds Hypoglycemic Activity. JFNS, 2017; 5(2): 23-27.View Article |
| [40] | Chang CLT, Liu HY, Kuo TF, Hsu YJ, Shen MY, Pan CY & Yang WC. Antidiabetic effect and mode of action of cytopiloyne. Evid Based Complement Alternat Med, 2013; 685642.View Article |
| [41] | Oluwole OO, & Oladunmoye MK. Phytochemical Screening and Antibacterial Activities of Bidens pilosa L. and Tridax procumbens L. on Skin Pathogens. Int J Modern Biol Med, 2017; 8(1): 24-26. |
| [42] | Bogucka-Kocka A, Zidorn C, Kasprzycka M, Szymczak G & Szewczyk K. Phenolic acid content, antioxidant and cytotoxic activities of four Kalanchoë species. Saudi J Bio Sci, 2018; 25(4): 622-630.View Article PubMed |
| [43] | Sharker SM, Hossain MK, Haque MR, Chowdhury AA, Kaisar A, Hasan CM & Rashid MA. Chemical and biological studies of Kalanchoe pinnata (Lam.) growing in Bangladesh. APJTB, 2012; 2(3): S1317-S1322.View Article |
| [44] | Shashank M, Khosla KK, Cathrin M & Debjit B. Preliminary Phytochemical Studies Of Kalanchoe pinnata (Lam.) Pers. J Med Plants Stud, 2013; 1(2): 19-23. |
| [45] | Vinayagam R, Jayachandran M & Xu B. Antidiabetic Effects of Simple Phenolic Acids: A Comprehensive Review. Phytother Res, 2016; 30(2): 184-199.View Article PubMed |
| [46] | Afolayan AJ & Sunmonu TO. Artemisia afra Jacq. ameliorates oxidative stress in the pancreas of streptozotocin-induced diabetic Wistar rats. Biosci Biotechnol Biochem, 2011; 75(11): 2083-2086.View Article PubMed |
