Studies on the Resistance of Cowpea Seeds (Vigna unguiculata) to Weevil (Callosobruchus maculatus) Infestations
1Department of Zoology, Nnamdi Azikiwe University, Awka
Three cowpea varieties namely: Kafanji, Brown variety and Ex-potiskum were used in this study to test their resistance on weevil infestation. Cowpea samples of 60grams were measured from each variety and were artificially infested with different weevil densities made up of male and female ratios of 5:10, 10:15 and 15:15. The samples had three replicates and a control treatment each. These were allowed to stand on the shelves without disturbance, thus making it possible for the weevils to mate and oviposit naturally. The results were analysed statistically and the differences between the treatments with respect to weight loss and adult emergence were significant (P>0.05) and the damages were also closely related to the infestation density increasing with the densities. Kafanji suffered more damage while the Brown variety and Ex-potiskum suffered less damage. This indicates that Ex-potiskum and Brown-variety exhibits more resistance qualities to the weevil attack than Kafanji which is more susceptible to the weevil attack. The physical as well as the chemical characteristics of the seeds were also found to influence the growth and development of the weevil forming the basis for resistance in the varieties. Breeding programmes utilizing the resistance characters in certain varieties are therefore suggested.
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Keywords: oviposition, grains, Ex potiskum, kafanji
American Journal of Zoological Research, 2014 2 (2),
Received February 04, 2013; Revised March 08, 2014; Accepted March 17, 2014Copyright: © 2014 Science and Education Publishing. All Rights Reserved.
Cite this article:
- C., Mogbo T., Okeke T. E., and Akunne C. E.. "Studies on the Resistance of Cowpea Seeds (Vigna unguiculata) to Weevil (Callosobruchus maculatus) Infestations." American Journal of Zoological Research 2.2 (2014): 37-40.
- C., M. T. , E., O. T. , & E., A. C. (2014). Studies on the Resistance of Cowpea Seeds (Vigna unguiculata) to Weevil (Callosobruchus maculatus) Infestations. American Journal of Zoological Research, 2(2), 37-40.
- C., Mogbo T., Okeke T. E., and Akunne C. E.. "Studies on the Resistance of Cowpea Seeds (Vigna unguiculata) to Weevil (Callosobruchus maculatus) Infestations." American Journal of Zoological Research 2, no. 2 (2014): 37-40.
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Cowpea is a warm weather crop that is well adapted to drier regions of the tropics like Nigeria where other food legumes do not thrive well . It is one of the most economically and nutritionally important indigenous African grain legumes produced throughout the tropical and subtropical areas of the world . Nigeria is its largest producer and consumer, accounting for about 45 percent of its world’s production [2, 3] while Africa accounts for about 75% . Cowpea seed pods and leaves are consumed in fresh form as green vegetables in some African countries , while the rest of the cowpea plant after the pods have been harvested serves as a nutritious fodder for livestock  and also a source of cash income when sold to farmers who use them as livestock feed. . Its nutritive value makes it an extremely important protein source to vegetarian and people who cannot afford animal protein . Cowpea seeds are also a rich source of minerals and vitamins . Cowpea is sometimes called “poor man’s meat” or “vegetable meat” by researchers due to its high protein content. Cowpea grain contains 23.4% protein, 1.8% fat and 60.3% carbohydrates and also a good source of vitamins and phosphorus .
However, the production and storage of this important food crop, Cowpea has faced so many constraints, such as diseases and the limited use of fertilizers and irrigation inputs  but insect pest is one of the major constraints. The major storage pest of cowpea is Callosobruchus maculatus . It infests cowpea before harvest, the higher the infestation levels before harvest, the greater the damage to the seeds in storage. This will result in higher weevil emergence causing a greater weight loss, larger number of holes and consequently loss of economic value . Infestations on stored grains may reach 50% within 3-4 months of storage . Striga gesnerioides, commonly called witch-weed, is also a major constraint to growth and productivity of cowpea (Vigna unguiculata L. Walp) throughout West Africa . If cowpea seeds are to be stored for longer periods, then it is advisable to treat the seeds with recommended insecticides .
Today, researches are focusing on the discovery of new therapeutic substances of natural origin, based on ethno medical and ethno veterinary practices with possible low or no toxicity to human, animal and environment .
One modest way of increasing food availability to cope with the Nigerian ever-increasing population at low cost is to protect what has been produced and to achieve this, plant materials that are inexpensive, safe to the environment, users and consumers alike, need to be exploited as suitable alternatives to the expensive, toxic and environmentally unsafe synthetic insecticides . Insecticides are widely used, and misused, to control storage insects, as evident in the Nigerian press reports on poison cases. Sudden deaths, blindness, and skin irritation are among the problems attributed to use of inappropriate storage chemicals in Nigeria . Control of insect with chemical insecticides has serious disadvantages such as the development of resistant strains, toxic residue, worker’s safety and increasing costs . The international institute of Tropical Agriculture (IITA) in Ibadan Nigeria is the center for worldwide collection and testing of cowpea germplasm. The IITA and collaborators are currently working on varietal improvement based on host plant resistant varieties that are ready for harvest in 60days.A breakthrough was achieved in 1995 when the resistance genes from wild cowpea species were successfully transferred into cultivated cowpea varieties including resistance to the storage weevil Callosobrochus maculatus, leafhopper and aphids using an in-vitro rescue techniques. Partial resistance to other pests and diseases has also been incorporated into elite varieties multiple resistances.
The main objective of the study is to investigate if different cowpea varieties have resistance to weevil infestation. Other objectives of the study are to determine the major nutrient composition in the different cowpea varieties through bio chemical analysis, study the development of Callosobruchus maculates on different varieties of cowpea (Kafanji, Ex-Potiskum and Brown Variety) at different population densities. The study is also aimed at determining the weight loss and damage in some cowpea varieties infested with C. maculatus.
2. Materials and Methods
The study was carried out in the laboratory of the Department of Zoology, Nnamdi Azikiwe University, Awka. Three cowpea varieties were used in this work and they were labeled thus: Kafanji, (Sample A), Brown variety (Sample B) and Ex-potiskum (Sample C). These varieties were purchased from a local farmer at Zamfara State. The clean uninfested cowpea grains were selected and heated in the oven at 100ºC for 5 minutes to ward off any stage of insect infestation. Sixty grams each of the cowpea varieties were measured into transparent containers with perforated lid covered with mosquito nets. Transparent containers were preferred because they aided clear observation of the insects. Callosobruchus maculatus was cultured in white transparent plastic containers covered with mosquito nets and its lids were perforated to prevent escape of the insect and entrance of other insects. Adult insects were used for the culture.
At the end of the culture period juvenile weevils were removed from the culture jars and introduced to the cowpea varieties with the use of an aspirator at different male /female densities of 5:10, 10:15 and 15:15 in three replicates. The sexes were separated into male and female by noting their morphological differences. Uninfested samples were maintained as control treatments for each variety and treatments were allowed to stand on the shelf undisturbed. The biochemical composition of the cowpea varieties was done at The National Agency for Food and Drug Administration and Control (NAFDAC) laboratory, Agulu Nigeria. One hundred grams seeds of each variety were taken for the analysis using the methods of AOAC .
Data collected at the end of the six weeks study were: total number of adult emergence, number of holed and unholed seeds and weight loss in seeds. Data generated from the study were subjected to analysis of variance (ANOVA) at (0.05) significant level with SPSS statistical package (version 19) and Duncan`s Multiple Range Test was used for means separation.
The population of adult emergence of C. maculatus at different population densities is presented in Table 1. Kafanji had more adult emergence than others (Plate 1). It was followed by Brown variety while Ex-potiskum had the least emergence of adult C. maculatus. The proximate composition of the cowpea varieties is reported in Table 2. The greatest weight loss in seeds was observed on Kafanji with mean value of 16.5 while the least weight loss was observed on Ex-potiskum with mean value of 1.0. The greatest weight loss was also obtained on the 15:15 density, indicating that weight loss increases with the insect density (Table 3). There is a significant difference (p<0.05) in the actual weight loss in the cowpea varieties. Test for means show that Brown variety and Ex-potiskum are statistically the same but statistically different from Kafanji variety. The percentage holed seeds was higher in Kafanji and least in Ex-potiskum (Table 4). The difference in the mean percentage holed seed was significant at p < 0.05, implying that Kafanji had the least resistance to weevil infestation.
The result of the present study has shown that some of the cowpea varieties evaluated show various degrees of resistance and susceptibility to adult C. maculatus attack. This supports the findings of Oke and Olajire,  who reported that the cowpea varieties studied exhibited some levels of resistance and susceptibility to Callosobruchus maculatus, a major storage insect pest of cowpea. From the results obtained, Ex-potiskum was found to be more resistant to C. maculatus followed by Brown variety and least resistance was observed in the Kafanji variety. It had least emergence of adult C. maculatus, least weight loss, and least percentage damaged seeds recorded. It has been reported that variables such as adult emergence, growth index, developmental period and weight loss are the most reliable indicators for resistance of cowpea to damage by C. maculatus [17, 18].
From the biochemical analysis carried out, Ex-potiskum and Brown variety contained high ash content which could be the cause of its high resistance to weevil infestation. Ash is known to possess insecticidal properties and is found effective in controlling storage insect pests, mainly Coleopterae [19, 20]. Giga,  reported the use of fine sand, wood ashes and plant materials with insecticidal properties by farmers to protect their products. Wolfson et al.  reported that a minimum ratio of 3 parts of ash to 4 parts of cowpeas prevented population growth of C. maculatus and that a 3 cm layer of ash on top of stored seeds prevented infestation by adults. They further stated that storing cowpeas with ash to protect them against Callosobruchus maculatus is a traditional storage method in Northern Cameroon. Moreso, the Kafanji variety had the highest protein content (21.8%) than the other two varieties studied. This could be the reason for its high adult C. maculatus emergence, high weight loss, and high percentage damaged seeds recorded. This implies that it has low resistance to weevil infestation. Research studies have shown that the increase of protein concentration in seed coat of cowpea may play a role towards susceptibility to weevil pest . Also  revealed that high protein content was linked to susceptibility to the stored- product insect infection.
Considering texture of the cowpea varieties, Kafanji possessed a smooth coat while the other two varieties are rough. This feature may be the reason for its low resistance to the weevil during the study period. This supports the findings of  who reported that C. maculatus prefers smooth-coated and well-filled seeds to their rough and wrinkled counterparts for oviposition.  indicated that seed coat texture plays significant role in inducing ovipositional response.
The study further reveals that cowpea variety with highest adult emergence (Kafanji) recorded extensive damage (i.e. higher percentage holed seeds and weight loss). In other words, increasing population of adult emergence causes more extensive damage and vice versa. This agrees with the findings of  who reported that the number of emerging adult determines the extent of damage, and consequently, seeds permitting more rapid and higher levels of adult emergence will be more extensively damaged. Statistically, the differences between the treatments with respect to adult emergence, percentage damage and actual weight loss were significant at (P<0.05).
The physical examination of the three cowpea varieties shows that there is difference in the size, texture, moisture content and seed testa colour. However, seed properties including seed testa colour, mass, size and moisture content generally do not influence the susceptibility of cowpea seeds and other cereals grains to C. maculatus and Sitophilus species in storage respectively [28, 29, 30].
The greatest weight loss was obtained on the 15:15 density indicating that it increases with the insect density. Seeds challenged with higher female: male ratios of bruchids were more damaged than those challenged with lower female: male. However, those challenged with equal male: female ratios were the most damaged in the cowpea varieties except in the Brown variety. This is in contrast to the findings of  who reported that seeds challenged with higher male: female ratios of bruchids were more damaged than those challenged with lower male: female or equal male: female ratios. Samples treated with more males than females caused severe damage, since a single female has been observed to mate several times with different males (depending on the number of times a female bruchid would mate in order to continue producing eggs).
Cowpea varieties can resist insect infestation to some extent even without the application of toxic chemical insecticides. Of the three cowpea varieties studied, Kafanji was more susceptible to adult C. maculatus attack hence would not be suitable for longer storage except insecticides are employed. This study rates Ex-potiskum higher than the other variety because of its low population of adult weevil emergence, weight loss, percentage whole seed and high ash content. It is highly recommended for both farmers and consumers but when not readily available, Brown variety should be used.
|||Abate, T.; Ajeigbe, H.; Boukar, O.; Fatokun, C.; Kamara, A.; Ndjeunga, J. and Ntare, B. (2011). Grain Legumes of Nigeria. Bulletin of Tropical Legume p4.|
|||Lowenberg-DeBoer, J. and Ibro, G. (2008) A Study Of The Cowpea Value Chain In Kano State, Nigeria, From A Pro-Poor And Gender Perspective. A paper commissioned by the GATE Project p16.|
|||Ndong A., Kébé K. H., Thiaw C. H., Diome T. and Sembène M. (2012). Genetic Distribution of the Cowpea (Vigna Unguiculata (L.) Walp) Bruchid (Callosobruchus maculatus F., Coleoptera, Bruchidae) Populations in Different Agro-Ecological Areas of West Africa. J Anim Sci Adv 2012, 2(7): 616-630.|
|||Brisibe, E. A, Adugbo, S. E, Ekanem, U, Brisibe, F. and Figueira, G. M (2011) Controlling Bruchid Pests of Stored Cowpea Seeds with Dried Leaves of Artemisia annua and Two Other Common Botanicals. African Journal of Biotechnology. 10(47), pp. 9586-9592.|
|||Ghaly A. E, and Alkoaik F. N (2010). Extraction of Protein from Common Plant Leaves for Use as Human Food. American Journal of Applied Sci. 7(3): 323-334.|
|||Abebe, G, Hattar, B, At-tawah, A. (2005). Nutrient availability as affected by manure application to cowpea (Vigna unguiculata L. Walp.) on Calacarious soils. Journal of Agric. Soc Sci. 1: 1-6.|
|||Dugje, I. Y., L. O. Omoigui, F Ekeleme, A. Y. Kamara, and H. Ajeigbe. (2009) Farmers’ Guide to Cowpea Production in West Africa. IITA, Ibadan, Nigeria. p20.|
|||Adeyemi, S. A.; Lewu, F. B; Adebola, P. O.; Bradley, G. and Okoh, A. I. (2012). Protein content variation in cowpea genotypes (Vigna unguiculata L. Walp.) grown in the Eastern Cape province of South Africa as affected by mineralised goat manure. African Journal of Agricultural Research Vol. 7(35), pp. 4943-4947.|
|||Hall A. E, Cisse N, Thiaw S, Elawad H. O. A, Ehlers J. D, (2003) Development of cowpea cultivars and germplasm by the Bean/Cowpea CRSP. Field Crops Res 82: 103-134.|
|||Baidoo, P. K.; Mochiah, M. B. and Owusu –Akyaw, M. (2010). The Effect of Time of Harvest on the Damage Caused by the Cowpea Weevil Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae) Journal of Stored Products and Postharvest Research Vol. 1(3), pp. 24-28.|
|||Asare, A. T., Bhavani S. Gowda, Isaac K. A. Galyuon, Lawrence M. Aboagye, Jemmy F. Takrama, Francis K. Padi, and Timko, M.P.(2011) Identification of Potential Sources of Striga Resistance In Cowpea [Vigna Unguiculata (L.) walp.] accessions from Ghana.|
|||Yeap, S. K., Ho W. Y., Beh B. K., Liang W. S., Ky H., Yousr A. H. N. and Alitheen N.B.(2010). Vernonia amygdalina, an Ethnoveterinary and Ethnomedical Used Green Vegetable with Multiple Bioactivities. Journal of Medicinal Plants Research, 4(25): 2787-2812.|
|||Magaji, B. T., Dike, M. C., Amatobi, C.I. and Onu, I. (2005). Comparative Efficacy of Some Plant Oils for the Control of Callosobruchus maculatus (F.), (Coleoptera: Bruchidae) on Stored Cowpea: M.Sc. Thesis, Ahmadu Bello University, Zaria, Nigeria.|
|||Association of Official Analytical Chemists (AOAC) (2010).|
|||Ofuya, T. I; Idoko, J. E. and Akintewe, L. A (2008). Ability of Sitophilus zeamais Motschulsky [Coleoptera: Curculonidae] from Four Locations In Nigeria To Infest And Damage Three Varieties Of Maize, Zea mays L. Nigerian Journal of Entomology 25:34-39|
|||Oke O. A and Olajire T. E. (2012). Determination of Insect Pests on Planted Cowpea Varities at Teaching and Research Farm of Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. Online International Journal of Microbiology Research, 1(1) pp. 1-7.|
|||Redden, R. J. and McGuire, J. (1983). The genetic evaluation of bruchid resistance in seeds of cowpea. Australian Journal of Agricultural Research 34, 707-715.|
|||Jackai, L. E. N. and Asante, S. K. (2003). A case for the standardization of protocols used in screening cowpea, Vigna unguiculata for resistance to Callosobruchus maculatus F. (Coleoptera: Bruchidae). Journal of Stored Products Research, 39:251-263.|
|||Gwinner J., Harnish R., Muck O. (1996): Manual on the Prevention of Post Harvest Grain Loss. GTZ, Eschborn.|
|||Swella G. B. and Mushobozy D. M. K. (2007): Evaluation of the efficacy of protectants against cowpea bruchids (Callosobruchus maculatus (F.)) on cowpea seeds (Vigna unguiculata (L.) Walp.). Plant Protect. Sci., 43:68-72.|
|||Giga, D. P. (1995). Progress in Bean Bruchid research in the SADC region: Journal of Appl Sci. in Southern Africa, 1, 107-112.|
|||Wolfson, J. L., Shade, R. E., Mentzer, P. E., and Murdock, L. L. (1991). Efficacy of ash for controlling infestations of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) in Stored Cowpeas. Journal of Stored Products Research, 27(4):239-243pp.|
|||Abdel-Sabour, A. G., Obiadalla-Ali, H. A., and AbdelRehim, K. A. (2010). Genetic and Chemical Analyses of six Cowpea and two Phaseolus Bean species differing in Resistance to Weevil Pest. J. Crop Sci. Biotech. 13(1):53-60.|
|||Khokhar M. and Gupta A. S. (1974). Relative resistance of some varietiesof wheat to Sitophilus oryza (L.) and Rhizoperthadominica (F.) at different temperatures. Bull. Grains Tech.12: 117-123pp|
|||Nwanze, K. F., Horber, E., Pitts, C. W. P. (1975). Evidence of ovipositional preference of Callosobruchus maculatus for cowpea varieties. Environmental Entomology. 4: 409-412.|
|||Akintola A .J, Oyegoke O. O. 2004. Physico-Chemical Properties of Ten Cowpea Lines on Resistance to Callosobruchus maculatus (Walp. ). Ethiop J. Sci. 27: 71-74|
|||Singh, B. B., Singh, S. R. and Adjadi, O. (1985). Bruchid resistance in cowpea. Crop Science 25, 736-739.|
|||Lale, N. E. S. and Kolo, A. A. (1998). Susceptibility of eight improved local cultivars of cowpea to Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) in Nigeria. International Journal of Pest Management 44: 25-27.|
|||Maina, Y. T. and Lale, N. E. S. (2005). Comparative resistance of local cultivars of sorghum and improved varieties of maize to Sitophilus zeamais infestation in storage. Nigerian Journal of Experimental and Applied Biology 6 (1): 61-64.|
|||Maina, Y. T. and Dlamini, C. G. (2009). Sreening of maize (Zea mays) varieties to maize weevil Sitophilus zeamais infestation in Swaziland. Uniswa Research Journal of Agriculture, Science and Technology, 12 (2): 83-88.|
|||Onyido, A. E.; Zeibe, C. C.; Okonkwo, N. J.; Ezugbo-Nwobi, I. K.; Egbuche, C. M.; Udemezue, O. I.; and Ezeanya, L. C. (2011). Damage Caused by the Bean Bruchid, Callosobruchus maculatus (Fabricius) on Different Legume Seeds on Sale in Awka and Onitsha Markets, Anambra State, South Eastern Nigeria. African Research Review: An International Multidisciplinary Journal, Ethiopia. 5(4):116-123pp.|