Open Access Peer-reviewed

Qualitative Analysis of Accessory Glands Secretory Proteins in Different Species of Drosophila

Suhasini L. Kudupali1, Shivanna N.2,

1Department of Zoology, Karnatak University, Dharwad, India

2Department of Applied Genetics, Karnatak University, Dharwad, India

American Journal of Zoological Research. 2013, 1(1), 1-4. DOI: 10.12691/ajzr-1-1-1
Published online: August 25, 2017

Abstract

Qualitative analysis of male accessory gland secretory proteins in 11 species of Drosophila was analyzed using SDS-PAGE technique and their molecular weight was calculated with the help of marker proteins, the molecular weight ranges from 12-134 kD. The accessory gland secretions of D. virilis and D. hydei reveal more number of protein fractions. D. rajasekari showed less number of ACP fractions. Out of 14 protein fractions of D. virilis 5 were glycosylated whereas in D. hydei none of the fractions were glycosylated. In D. rajasekari 2 fractions were glycosylated. In D. bipectinata, D. malerkotlina and D. ananassae even though the accessory glands produced 7, 8 and 11 protein fractions only one protein fraction is glycosylated. These results showed that the number of ACP fractions and their glycosylation is species specific.

Keywords:

drosophila, accessory gland, qualitative analysis, CBB stain, glycosylated fractions, PAS stain
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