Open Access Peer-reviewed

Detection and Quantitative Analysis for 2-Thiobarbituric Acid Utilizing Uv-Visible Spectrophotometer

Ronald Bartzatt1,, Maggie Bartlett1, Nancy Handler1

1Department of Chemistry, Durham Science Center, University of Nebraska, Omaha, USA

American Journal of Pharmacological Sciences. 2013, 1(1), 10-14. DOI: 10.12691/ajps-1-1-3
Published online: August 25, 2017

Abstract

The thiobarbiturates differ from other barbiturates in having a sulfur atom in place of the carbonyl oxygen at the C-2 position. The drug 2-thiobarbituric acid was found to be sufficiently soluble in 95% ethanol and 5% water for highly sensitive detection and assay. Analytical samples, including standards, were studied at 320 nanometer wavelength with background absorbance of the solvent correctly determined and subtracted from prepared specimens in one centimeter glass cuvettes. An ultraviolet-visible instrument Spectronic 21D was found to be suitable for this assay. The molar extinction coefficient was determined at 320nm by dissolving a known amount of 2-thiobarbituric acid into 95% ethanol and 5% water and found to be 414.9 Liter/(cm)(mole). An absorbance spectrum for 2-thiobarbituric acid is presented. The standard curve utilized a range from 0.00006267 molar to 0.004201 molar. The lowest concentration assayed was0.01313 grams per liter (1.313E-05 grams per milliliter or 13.13 micrograms per milliliter) with the highest concentration at 0.6056 grams per liter (6.056E-04 grams per milliliter or 605.6 micrograms per milliliter). This method was found to be consistent and highly sensitive.

Keywords:

2-thiobarbituric acid, Bathyran, 2-Thio-4, 6-dioxypyrimidine, sedative, hypnotic
[1]  Goodman, L.S. and Gilman, A, Pharmacological Basis of Therapeutics, The MacMillan Company, London, 1970, 98-132.
 
[2]  Mushin, W.W, The thiobarbiturates,”Br. Med. J., 1(4982).1532-1553. June.1956.
 
[3]  Bamanie, F., Shehata, A., Moustafa, M. and Mashaly, M.M, “Green chemistry 1: simple and efficient synthesis in water and antibacterial activity of 5-arylidene derivatives of thiobarbituric and barbituric acids,” J. American Science, 8(1). 481-485. 2012.
 
[4]  Bauhlel, A., Curti, D. and Vanelle, P, “New methodology for the synthesis of thiobarbiturates mediated by manganese(III) acetate,” Molecules, 17(4). 4313-4325. April.2012.
 
[5]  Lee, J.H., Lee, S., Park, M.Y. and Myung, H, “Characterization of thiobarbituric acid derivatives as inhibitors of hepatitis C virus NS5B polymerase,” Virol. J., 8(18).1-4. Jan.2011.
 
[6]  Yin, S., Si, Y., Wang, S., Oh, S., Lee, S., Sim, S., Yang, J., Qian, G., Lee. J. and Park, Y, “The effect of thiobarbituric acid on tyrosinase: inhibition kinetics and computational simulation,” J. Biomol. Struct.Dyn., 29(3). 463-470. Dec.2011.View Article  PubMed
 
[7]  Pryor, W, “The antioxidant nutrients and disease prevention-what, do we know and what do we need to find out?” Am. J. Clin. Nulr., 53(1 suppl). 391S-393S. 1991. PubMed
 
[8]  Janero, D.R, “Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury,” Free Radic. Biol. Med., 9(6). 515-540. 1990.View Article
 
[9]  Oldham, K.M. and Bowen, P.E, “Oxidative stree in critical care: is antioxidant supplementation beneficial?,” J. Am. Diet Assoc., 98(9). 1001-1008. Sep.1998.View Article
 
[10]  Williams, L.A., Hardy, A.T., Cohen, J.S. and Zak, B, “Ultraviolet differential spectrophotometry of thiobarbiturates,” J. Medicinal Pharmaceutical Chem., 2(6). 609-615.1960.
 
[11]  Gruhl, H, “Methods for detecting barbiturate poisoning in a clinical laboratory,” J. Clinical Chem. CliinicalBiochem, 27(2). 53-56. 1989.
 
[12]  Lipinski, C.A., Lombardo, F., Dominy, B.W. and Feeney, P.J, “Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings,” Adv. Drug Del. Rev., 46. 3-26. 2001.View Article
 
[13]  Stephens, M.A, “Use of the Kolmogorov-Smirnov, Cramer-von Mises and related statisticswithout extensive tables,”J. of the Royal Statistical Society, Series B 32. 115-122. 1970.
 
[14]  Davis, J.C, Statistics and Data Analysis in Geology, John Wiley & Sons, New York, 1986, 22-98.
 
[15]  Hammer O. and Harper, D.A, Paleontological Data Analysis, Wiley Online Library, 2007. [E-book] Available: Wiley netlibrary e-book.