Figure 3. Immunohistochemistery (IHC) for Evaluation of TNF-α expression on spleen tissue. Immunohistochemical analysis of TNF-α expressions in spleens of untreated control group (A), methotrexate-treated group (B), papaya-treated group (C), kiwifruit-treated group (D). High expression of TNF-α was seen in the methotrexate-treated group (strong staining, 111). Meanwhile, the lowest expression of TNF-α was found in group treated with methotrexate and kiwifruit (weak staining, 1). TNF-α expression was indicated with black arrows. Stained by DAB -chromogen (Brown color) immunostaining.

From

Immunomodulatory Effect of Carica Papaya and Actinidia Delicosa on Methotrexate Induced Immunosuppression in Male Rats

Amina A. Bedawy, Ibrahim A. Ibrahim, Sherif Y. Saleh, Abeir A. Shalaby, Marwa A. El-Beltagy

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